Tumor bioengineering using a transglutaminase crosslinked hydrogel.
Development of a physiologically relevant 3D model system for cancer research and drug development is a current challenge. We have adopted a 3D culture system based on a transglutaminase-crosslinked gelatin gel (Col-Tgel) to mimic the tumor 3D microenvironment. The system has several unique advantag...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4136878?pdf=render |
| _version_ | 1818854093383270400 |
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| author | Josephine Y Fang Shih-Jye Tan Zhi Yang Charisse Tayag Bo Han |
| author_facet | Josephine Y Fang Shih-Jye Tan Zhi Yang Charisse Tayag Bo Han |
| author_sort | Josephine Y Fang |
| collection | DOAJ |
| description | Development of a physiologically relevant 3D model system for cancer research and drug development is a current challenge. We have adopted a 3D culture system based on a transglutaminase-crosslinked gelatin gel (Col-Tgel) to mimic the tumor 3D microenvironment. The system has several unique advantages over other alternatives including presenting cell-matrix interaction sites from collagen-derived peptides, geometry-initiated multicellular tumor spheroids, and metabolic gradients in the tumor microenvironment. Also it provides a controllable wide spectrum of gel stiffness for mechanical signals, and technical compatibility with imaging based screening due to its transparent properties. In addition, the Col-Tgel provides a cure-in-situ delivery vehicle for tumor xenograft formation in animals enhancing tumor cell uptake rate. Overall, this distinctive 3D system could offer a platform to more accurately mimic in vivo situations to study tumor formation and progression both in vitro and in vivo. |
| first_indexed | 2024-12-19T07:47:14Z |
| format | Article |
| id | doaj.art-194be2ca2cf04ec5bee4c41892144ca7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T07:47:14Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-194be2ca2cf04ec5bee4c41892144ca72022-12-21T20:30:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10561610.1371/journal.pone.0105616Tumor bioengineering using a transglutaminase crosslinked hydrogel.Josephine Y FangShih-Jye TanZhi YangCharisse TayagBo HanDevelopment of a physiologically relevant 3D model system for cancer research and drug development is a current challenge. We have adopted a 3D culture system based on a transglutaminase-crosslinked gelatin gel (Col-Tgel) to mimic the tumor 3D microenvironment. The system has several unique advantages over other alternatives including presenting cell-matrix interaction sites from collagen-derived peptides, geometry-initiated multicellular tumor spheroids, and metabolic gradients in the tumor microenvironment. Also it provides a controllable wide spectrum of gel stiffness for mechanical signals, and technical compatibility with imaging based screening due to its transparent properties. In addition, the Col-Tgel provides a cure-in-situ delivery vehicle for tumor xenograft formation in animals enhancing tumor cell uptake rate. Overall, this distinctive 3D system could offer a platform to more accurately mimic in vivo situations to study tumor formation and progression both in vitro and in vivo.http://europepmc.org/articles/PMC4136878?pdf=render |
| spellingShingle | Josephine Y Fang Shih-Jye Tan Zhi Yang Charisse Tayag Bo Han Tumor bioengineering using a transglutaminase crosslinked hydrogel. PLoS ONE |
| title | Tumor bioengineering using a transglutaminase crosslinked hydrogel. |
| title_full | Tumor bioengineering using a transglutaminase crosslinked hydrogel. |
| title_fullStr | Tumor bioengineering using a transglutaminase crosslinked hydrogel. |
| title_full_unstemmed | Tumor bioengineering using a transglutaminase crosslinked hydrogel. |
| title_short | Tumor bioengineering using a transglutaminase crosslinked hydrogel. |
| title_sort | tumor bioengineering using a transglutaminase crosslinked hydrogel |
| url | http://europepmc.org/articles/PMC4136878?pdf=render |
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