Automated Light Transmission Aggregometry with and without Platelet Poor Plasma Reference: A Method Comparison
Background Light transmission aggregometry (LTA) is considered the gold standard for the evaluation of platelet function but is labor-intensive and involves numerous manual steps. Automation may contribute to standardization. Here, we evaluate the performance characteristics of a new automated instr...
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| Format: | Article |
| Language: | English |
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Georg Thieme Verlag KG
2023-01-01
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| Series: | TH Open |
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| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0043-1762588 |
| _version_ | 1828009606348013568 |
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| author | Ulrich J. Sachs Lida Röder Nina Cooper Christian Radon Hans-Jürgen Kolde |
| author_facet | Ulrich J. Sachs Lida Röder Nina Cooper Christian Radon Hans-Jürgen Kolde |
| author_sort | Ulrich J. Sachs |
| collection | DOAJ |
| description | Background Light transmission aggregometry (LTA) is considered the gold standard for the evaluation of platelet function but is labor-intensive and involves numerous manual steps. Automation may contribute to standardization. Here, we evaluate the performance characteristics of a new automated instrument, Thrombomate XRA (TXRA), and compare it against a manual instrument (PAP-8).
Materials and Methods Leftover blood samples from blood donors or patients were tested in parallel with identical reagents and in identical concentrations both manually using PAP-8 and automated on the TXRA. In addition to precision and method comparison, an additional evaluation was performed on the TXRA against “virtual” platelet-poor plasma (VPPP) based on artificial intelligence. The main focus was on comparing the maximum aggregation (MA%) values.
Results Precision for MA% ranged from 1.4 to 4.6% on TXRA for all reagents. Normal ranges for 100 healthy blood donors on both instruments were in a similar range for all reagents, with a tendency to slightly higher values with TXRA. Most agonists resulted in normally distributed MA%. Comparing 47 patient samples on both devices showed a good correlation for both slope and MA% with some differences in individual samples with epinephrine and TRAP. Correlation between the TXRA measurement against PPP and “virtual” PPP demonstrated excellent correlation. Reaction signatures of both devices were very similar.
Conclusion TXRA provides reproducible LTA results that correlate with an established manual method when tested against PPP or VPPP. Its ability to perform LTA only from platelet-rich plasma without requiring autologous PPP simplifies LTA. TXRA is an important step not only for further standardizing LTA but also for a more widespread use of this important method. |
| first_indexed | 2024-04-10T08:41:08Z |
| format | Article |
| id | doaj.art-194dc8fac08042239017caa2a4acb2e5 |
| institution | Directory Open Access Journal |
| issn | 2512-9465 |
| language | English |
| last_indexed | 2024-04-10T08:41:08Z |
| publishDate | 2023-01-01 |
| publisher | Georg Thieme Verlag KG |
| record_format | Article |
| series | TH Open |
| spelling | doaj.art-194dc8fac08042239017caa2a4acb2e52023-02-23T00:07:33ZengGeorg Thieme Verlag KGTH Open2512-94652023-01-010701e56e6410.1055/s-0043-1762588Automated Light Transmission Aggregometry with and without Platelet Poor Plasma Reference: A Method ComparisonUlrich J. Sachs0Lida Röder1Nina Cooper2Christian Radon3Hans-Jürgen Kolde4Department of Thrombosis and Haemostasis, Giessen University Hospital, Giessen, GermanyInstitute for Clinical Immunology, Transfusion Medicine, and Haemostaseology, Justus Liebig University, Giessen, GermanyInstitute for Clinical Immunology, Transfusion Medicine, and Haemostaseology, Justus Liebig University, Giessen, GermanyBehnk Elektronik, Norderstedt, GermanyConsulting Diagnostics, Seefeld, GermanyBackground Light transmission aggregometry (LTA) is considered the gold standard for the evaluation of platelet function but is labor-intensive and involves numerous manual steps. Automation may contribute to standardization. Here, we evaluate the performance characteristics of a new automated instrument, Thrombomate XRA (TXRA), and compare it against a manual instrument (PAP-8). Materials and Methods Leftover blood samples from blood donors or patients were tested in parallel with identical reagents and in identical concentrations both manually using PAP-8 and automated on the TXRA. In addition to precision and method comparison, an additional evaluation was performed on the TXRA against “virtual” platelet-poor plasma (VPPP) based on artificial intelligence. The main focus was on comparing the maximum aggregation (MA%) values. Results Precision for MA% ranged from 1.4 to 4.6% on TXRA for all reagents. Normal ranges for 100 healthy blood donors on both instruments were in a similar range for all reagents, with a tendency to slightly higher values with TXRA. Most agonists resulted in normally distributed MA%. Comparing 47 patient samples on both devices showed a good correlation for both slope and MA% with some differences in individual samples with epinephrine and TRAP. Correlation between the TXRA measurement against PPP and “virtual” PPP demonstrated excellent correlation. Reaction signatures of both devices were very similar. Conclusion TXRA provides reproducible LTA results that correlate with an established manual method when tested against PPP or VPPP. Its ability to perform LTA only from platelet-rich plasma without requiring autologous PPP simplifies LTA. TXRA is an important step not only for further standardizing LTA but also for a more widespread use of this important method.http://www.thieme-connect.de/DOI/DOI?10.1055/s-0043-1762588light transmission aggregometryplatelet functionprecisionreproducibilityreference ranges |
| spellingShingle | Ulrich J. Sachs Lida Röder Nina Cooper Christian Radon Hans-Jürgen Kolde Automated Light Transmission Aggregometry with and without Platelet Poor Plasma Reference: A Method Comparison TH Open light transmission aggregometry platelet function precision reproducibility reference ranges |
| title | Automated Light Transmission Aggregometry with and without Platelet Poor Plasma Reference: A Method Comparison |
| title_full | Automated Light Transmission Aggregometry with and without Platelet Poor Plasma Reference: A Method Comparison |
| title_fullStr | Automated Light Transmission Aggregometry with and without Platelet Poor Plasma Reference: A Method Comparison |
| title_full_unstemmed | Automated Light Transmission Aggregometry with and without Platelet Poor Plasma Reference: A Method Comparison |
| title_short | Automated Light Transmission Aggregometry with and without Platelet Poor Plasma Reference: A Method Comparison |
| title_sort | automated light transmission aggregometry with and without platelet poor plasma reference a method comparison |
| topic | light transmission aggregometry platelet function precision reproducibility reference ranges |
| url | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0043-1762588 |
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