Genomic Analysis of <i>Pseudomonas asiatica</i> JP233: An Efficient Phosphate-Solubilizing Bacterium
The bacterium <i>Pseudomonas</i> sp. strain JP233 has been reported to efficiently solubilize sparingly soluble inorganic phosphate, promote plant growth and significantly reduce phosphorus (P) leaching loss from soil. The production of 2-keto gluconic acid (2KGA) by strain JP233 was ide...
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MDPI AG
2022-12-01
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| author | Linlin Wang Fangyuan Zhou Jianbo Zhou Paul R. Harvey Haiyang Yu Guangzhi Zhang Xinjian Zhang |
| author_facet | Linlin Wang Fangyuan Zhou Jianbo Zhou Paul R. Harvey Haiyang Yu Guangzhi Zhang Xinjian Zhang |
| author_sort | Linlin Wang |
| collection | DOAJ |
| description | The bacterium <i>Pseudomonas</i> sp. strain JP233 has been reported to efficiently solubilize sparingly soluble inorganic phosphate, promote plant growth and significantly reduce phosphorus (P) leaching loss from soil. The production of 2-keto gluconic acid (2KGA) by strain JP233 was identified as the main active metabolite responsible for phosphate solubilization. However, the genetic basis of phosphate solubilization and plant-growth promotion remained unclear. As a result, the genome of JP233 was sequenced and analyzed in this study. The JP233 genome consists of a circular chromosome with a size of 5,617,746 bp and a GC content of 62.86%. No plasmids were detected in the genome. There were 5097 protein-coding sequences (CDSs) predicted in the genome. Phylogenetic analyses based on genomes of related <i>Pseudomonas</i> spp. identified strain JP233 as <i>Pseudomonas asiatica</i>. Comparative pangenomic analysis among 9 <i>P. asiatica</i> strains identified 4080 core gene clusters and 111 singleton genes present only in JP233. Genes associated with 2KGA production detected in strain JP233, included those encoding glucose dehydrogenase, pyrroloquinoline quinone and gluoconate dehydrogenase. Genes associated with mechanisms of plant-growth promotion and nutrient acquisition detected in JP233 included those involved in IAA biosynthesis, ethylene catabolism and siderophore production. Numerous genes associated with other properties beneficial to plant growth were also detected in JP233, included those involved in production of acetoin, 2,3-butanediol, trehalose, and resistance to heavy metals. This study provides the genetic basis to elucidate the plant-growth promoting and bio-remediation properties of strain JP233 and its potential applications in agriculture and industry. |
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| spelling | doaj.art-194df3433d4447d1b71dd8a6ec61ac9b2023-11-24T15:04:22ZengMDPI AGGenes2073-44252022-12-011312229010.3390/genes13122290Genomic Analysis of <i>Pseudomonas asiatica</i> JP233: An Efficient Phosphate-Solubilizing BacteriumLinlin Wang0Fangyuan Zhou1Jianbo Zhou2Paul R. Harvey3Haiyang Yu4Guangzhi Zhang5Xinjian Zhang6Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan 250103, ChinaShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan 250103, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, ChinaShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan 250103, ChinaShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan 250103, ChinaShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan 250103, ChinaShandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan 250103, ChinaThe bacterium <i>Pseudomonas</i> sp. strain JP233 has been reported to efficiently solubilize sparingly soluble inorganic phosphate, promote plant growth and significantly reduce phosphorus (P) leaching loss from soil. The production of 2-keto gluconic acid (2KGA) by strain JP233 was identified as the main active metabolite responsible for phosphate solubilization. However, the genetic basis of phosphate solubilization and plant-growth promotion remained unclear. As a result, the genome of JP233 was sequenced and analyzed in this study. The JP233 genome consists of a circular chromosome with a size of 5,617,746 bp and a GC content of 62.86%. No plasmids were detected in the genome. There were 5097 protein-coding sequences (CDSs) predicted in the genome. Phylogenetic analyses based on genomes of related <i>Pseudomonas</i> spp. identified strain JP233 as <i>Pseudomonas asiatica</i>. Comparative pangenomic analysis among 9 <i>P. asiatica</i> strains identified 4080 core gene clusters and 111 singleton genes present only in JP233. Genes associated with 2KGA production detected in strain JP233, included those encoding glucose dehydrogenase, pyrroloquinoline quinone and gluoconate dehydrogenase. Genes associated with mechanisms of plant-growth promotion and nutrient acquisition detected in JP233 included those involved in IAA biosynthesis, ethylene catabolism and siderophore production. Numerous genes associated with other properties beneficial to plant growth were also detected in JP233, included those involved in production of acetoin, 2,3-butanediol, trehalose, and resistance to heavy metals. This study provides the genetic basis to elucidate the plant-growth promoting and bio-remediation properties of strain JP233 and its potential applications in agriculture and industry.https://www.mdpi.com/2073-4425/13/12/2290phosphate-solubilizing bacteria<i>Pseudomonas asiatica</i>genome analysisplant-growth promotion |
| spellingShingle | Linlin Wang Fangyuan Zhou Jianbo Zhou Paul R. Harvey Haiyang Yu Guangzhi Zhang Xinjian Zhang Genomic Analysis of <i>Pseudomonas asiatica</i> JP233: An Efficient Phosphate-Solubilizing Bacterium Genes phosphate-solubilizing bacteria <i>Pseudomonas asiatica</i> genome analysis plant-growth promotion |
| title | Genomic Analysis of <i>Pseudomonas asiatica</i> JP233: An Efficient Phosphate-Solubilizing Bacterium |
| title_full | Genomic Analysis of <i>Pseudomonas asiatica</i> JP233: An Efficient Phosphate-Solubilizing Bacterium |
| title_fullStr | Genomic Analysis of <i>Pseudomonas asiatica</i> JP233: An Efficient Phosphate-Solubilizing Bacterium |
| title_full_unstemmed | Genomic Analysis of <i>Pseudomonas asiatica</i> JP233: An Efficient Phosphate-Solubilizing Bacterium |
| title_short | Genomic Analysis of <i>Pseudomonas asiatica</i> JP233: An Efficient Phosphate-Solubilizing Bacterium |
| title_sort | genomic analysis of i pseudomonas asiatica i jp233 an efficient phosphate solubilizing bacterium |
| topic | phosphate-solubilizing bacteria <i>Pseudomonas asiatica</i> genome analysis plant-growth promotion |
| url | https://www.mdpi.com/2073-4425/13/12/2290 |
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