A Study on molecular characterization of Razi Bacillus anthracis Sterne 34F2 substrain in Iran
Anthrax, a zoonotic disease caused by Bacillus anthracis, has affected humans since ancient times. For genomic characterization of Razi B. anthracis Sterne 34F2 substrain, single nucleotide polymorphism (SNP) genotyping method developed by Van Erth, variable-number tandem-repeat (VNTR)-8 analysis...
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| Format: | Article |
| Language: | English |
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Razi Vaccine and Serum Research Institute
2016-07-01
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| Series: | Archives of Razi Institute |
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| Online Access: | http://www.archrazi.com/article_106445.html |
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| author | Tadayon, K. Moazeni Jula, G. Banihashemi, R., Sekhavati, M. Naseri Rad Razaz, H. |
| author_facet | Tadayon, K. Moazeni Jula, G. Banihashemi, R., Sekhavati, M. Naseri Rad Razaz, H. |
| author_sort | Tadayon, K. |
| collection | DOAJ |
| description | Anthrax, a zoonotic disease caused by Bacillus anthracis, has affected humans since ancient times. For genomic
characterization of Razi B. anthracis Sterne 34F2 substrain, single nucleotide polymorphism (SNP) genotyping
method developed by Van Erth, variable-number tandem-repeat (VNTR)-8 analysis proposed by Keim, and
multiple-locus VNTR analysis (MLVA)-3 introduced by Levy were employed. In the SNPs typing system,
where the nucleotide content of the genome at 13 evolutionary canonical loci was collectively analyzed, the
originally South African 34F2 substrain was categorized in the A.Br.001/002 subgroup. In the VNTR-8 analysis,
fragments with lengths of 314, 229, 162, 580, 532, 158, and 137 bp were identified at the following loci: vrrA,
vrrB1, vrrB2, vrrC1, vrrC2, CG3, and pxO1, respectively. In addition, application of Levy's MLVA-3
genotyping method revealed that the genome of this strain carried 941, 451, and 864 bp fragments at AA03,
AJ03, and AA07 loci, respectively. The present findings are undoubtedly helpful in meeting the requirements set
by the World Organization for Animal Health (OIE) and World Health Organization (WHO) for anthrax vaccine
manufacturers including Razi Institute. However, further similar studies are required to promote the current
epidemiological knowledge of anthrax in Iran. |
| first_indexed | 2024-04-13T17:11:33Z |
| format | Article |
| id | doaj.art-19512e19617d4908be705177aca52505 |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-04-13T17:11:33Z |
| publishDate | 2016-07-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-19512e19617d4908be705177aca525052022-12-22T02:38:15ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722016-07-017128186A Study on molecular characterization of Razi Bacillus anthracis Sterne 34F2 substrain in IranTadayon, K.0Moazeni Jula, G.1Banihashemi, R.,2Sekhavati, M.3Naseri Rad4Razaz, H.5Department of Veterinary Aerobic Bacteria, Razi Vaccine and Serum Research Institute,Education and Extension Organization, Karaj, IranDepartment of Veterinary Aerobic Bacteria, Razi Vaccine and Serum Research Institute,Education and Extension Organization, Karaj, IranDepartment of Veterinary Aerobic Bacteria, Razi Vaccine and Serum Research Institute,Education and Extension Organization, Karaj, IranDepartment of Veterinary Aerobic Bacteria, Razi Vaccine and Serum Research Institute,Education and Extension Organization, Karaj, IranDepartment of Veterinary Aerobic Bacteria, Razi Vaccine and Serum Research Institute,Education and Extension Organization, Karaj, IranDepartment of Veterinary Aerobic Bacteria, Razi Vaccine and Serum Research Institute,Education and Extension Organization, Karaj, IranAnthrax, a zoonotic disease caused by Bacillus anthracis, has affected humans since ancient times. For genomic characterization of Razi B. anthracis Sterne 34F2 substrain, single nucleotide polymorphism (SNP) genotyping method developed by Van Erth, variable-number tandem-repeat (VNTR)-8 analysis proposed by Keim, and multiple-locus VNTR analysis (MLVA)-3 introduced by Levy were employed. In the SNPs typing system, where the nucleotide content of the genome at 13 evolutionary canonical loci was collectively analyzed, the originally South African 34F2 substrain was categorized in the A.Br.001/002 subgroup. In the VNTR-8 analysis, fragments with lengths of 314, 229, 162, 580, 532, 158, and 137 bp were identified at the following loci: vrrA, vrrB1, vrrB2, vrrC1, vrrC2, CG3, and pxO1, respectively. In addition, application of Levy's MLVA-3 genotyping method revealed that the genome of this strain carried 941, 451, and 864 bp fragments at AA03, AJ03, and AA07 loci, respectively. The present findings are undoubtedly helpful in meeting the requirements set by the World Organization for Animal Health (OIE) and World Health Organization (WHO) for anthrax vaccine manufacturers including Razi Institute. However, further similar studies are required to promote the current epidemiological knowledge of anthrax in Iran.http://www.archrazi.com/article_106445.htmlIranAnthraxMax Sterne |
| spellingShingle | Tadayon, K. Moazeni Jula, G. Banihashemi, R., Sekhavati, M. Naseri Rad Razaz, H. A Study on molecular characterization of Razi Bacillus anthracis Sterne 34F2 substrain in Iran Archives of Razi Institute Iran Anthrax Max Sterne |
| title | A Study on molecular characterization of Razi Bacillus anthracis Sterne 34F2 substrain in Iran |
| title_full | A Study on molecular characterization of Razi Bacillus anthracis Sterne 34F2 substrain in Iran |
| title_fullStr | A Study on molecular characterization of Razi Bacillus anthracis Sterne 34F2 substrain in Iran |
| title_full_unstemmed | A Study on molecular characterization of Razi Bacillus anthracis Sterne 34F2 substrain in Iran |
| title_short | A Study on molecular characterization of Razi Bacillus anthracis Sterne 34F2 substrain in Iran |
| title_sort | study on molecular characterization of razi bacillus anthracis sterne 34f2 substrain in iran |
| topic | Iran Anthrax Max Sterne |
| url | http://www.archrazi.com/article_106445.html |
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