Overexpression of RtSYP121 confers cadmium colerance by promoting vesicle trafficking, maintaining ion homeostasis, and alleviating photosynthetic inhibition in Arabidopsis
Cadmium (Cd) is a toxic heavy metal in soil that seriously threatens crop production, food security, and human health. Syntaxins, a prototype family of Soluble N-ethyl-maleimide-associated protein receptors (SNAREs) involved in vesicle trafficking, are implicated in resistance to abiotic stresses, i...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323001240 |
| _version_ | 1797902937299615744 |
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| author | Jiayuan Zhang Miao Zhang Jian Zhang Fang Wang Yingchun Wang Linlin Zheng |
| author_facet | Jiayuan Zhang Miao Zhang Jian Zhang Fang Wang Yingchun Wang Linlin Zheng |
| author_sort | Jiayuan Zhang |
| collection | DOAJ |
| description | Cadmium (Cd) is a toxic heavy metal in soil that seriously threatens crop production, food security, and human health. Syntaxins, a prototype family of Soluble N-ethyl-maleimide-associated protein receptors (SNAREs) involved in vesicle trafficking, are implicated in resistance to abiotic stresses, including Cd stress, but the molecular mechanisms underlying the involvement of syntaxins in Cd tolerance in plants are unclear. In this study, we isolated and functionally characterized the syntaxin gene RtSYP121 from Reaumuria trigyna to evaluate its potential for phytoremediation. RtSYP121 resides in the plasma membrane. The transcriptional level of RtSYP121 was strongly increased by salt, drought, and Cd stress. Overexpression of RtSYP121 significantly enhanced the Cd tolerance of transgenic Arabidopsis. The Cd tolerance of transgenic plants mainly depended on elevated vesicle trafficking, which increased the content of K+ and Ca2+ and thus decreased the accumulation of Cd2+ by regulating the delivery or activity of ion transporters, channels, and pumps. Moreover, overexpression of RtSYP121 in Arabidopsis ameliorated Cd stress–induced phytotoxic effects, including growth inhibition, ROS burst, photosynthetic impairment, and cell death. Therefore, we suggest that RtSYP121 plays multiple roles in the plant response to Cd stress by promoting vesicle trafficking, maintaining ion homeostasis, and alleviating photosynthetic inhibition. |
| first_indexed | 2024-04-10T09:25:06Z |
| format | Article |
| id | doaj.art-1952f0e90393425db08e32bddd92ad12 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-10T09:25:06Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-1952f0e90393425db08e32bddd92ad122023-02-20T04:08:43ZengElsevierEcotoxicology and Environmental Safety0147-65132023-03-01252114620Overexpression of RtSYP121 confers cadmium colerance by promoting vesicle trafficking, maintaining ion homeostasis, and alleviating photosynthetic inhibition in ArabidopsisJiayuan Zhang0Miao Zhang1Jian Zhang2Fang Wang3Yingchun Wang4Linlin Zheng5Key Laboratory of Forage and Endemic Crop Biotechnology, Minister of Education, School of Life Sciences, Inner Mongolia University, Hohhot, China; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Hohhot, ChinaKey Laboratory of Forage and Endemic Crop Biotechnology, Minister of Education, School of Life Sciences, Inner Mongolia University, Hohhot, China; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Hohhot, ChinaKey Laboratory of Forage and Endemic Crop Biotechnology, Minister of Education, School of Life Sciences, Inner Mongolia University, Hohhot, China; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Hohhot, ChinaKey Laboratory of Forage and Endemic Crop Biotechnology, Minister of Education, School of Life Sciences, Inner Mongolia University, Hohhot, China; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Hohhot, ChinaKey Laboratory of Forage and Endemic Crop Biotechnology, Minister of Education, School of Life Sciences, Inner Mongolia University, Hohhot, China; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Hohhot, ChinaKey Laboratory of Forage and Endemic Crop Biotechnology, Minister of Education, School of Life Sciences, Inner Mongolia University, Hohhot, China; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Hohhot, China; Corresponding author at: Key Laboratory of Forage and Endemic Crop Biotechnology, Minister of Education, School of Life Sciences, Inner Mongolia University, Hohhot, China.Cadmium (Cd) is a toxic heavy metal in soil that seriously threatens crop production, food security, and human health. Syntaxins, a prototype family of Soluble N-ethyl-maleimide-associated protein receptors (SNAREs) involved in vesicle trafficking, are implicated in resistance to abiotic stresses, including Cd stress, but the molecular mechanisms underlying the involvement of syntaxins in Cd tolerance in plants are unclear. In this study, we isolated and functionally characterized the syntaxin gene RtSYP121 from Reaumuria trigyna to evaluate its potential for phytoremediation. RtSYP121 resides in the plasma membrane. The transcriptional level of RtSYP121 was strongly increased by salt, drought, and Cd stress. Overexpression of RtSYP121 significantly enhanced the Cd tolerance of transgenic Arabidopsis. The Cd tolerance of transgenic plants mainly depended on elevated vesicle trafficking, which increased the content of K+ and Ca2+ and thus decreased the accumulation of Cd2+ by regulating the delivery or activity of ion transporters, channels, and pumps. Moreover, overexpression of RtSYP121 in Arabidopsis ameliorated Cd stress–induced phytotoxic effects, including growth inhibition, ROS burst, photosynthetic impairment, and cell death. Therefore, we suggest that RtSYP121 plays multiple roles in the plant response to Cd stress by promoting vesicle trafficking, maintaining ion homeostasis, and alleviating photosynthetic inhibition.http://www.sciencedirect.com/science/article/pii/S0147651323001240SyntaxinVesicle traffickingRtSYP121Cadmium toleranceIon homeostasisPhotosynthetic inhibition |
| spellingShingle | Jiayuan Zhang Miao Zhang Jian Zhang Fang Wang Yingchun Wang Linlin Zheng Overexpression of RtSYP121 confers cadmium colerance by promoting vesicle trafficking, maintaining ion homeostasis, and alleviating photosynthetic inhibition in Arabidopsis Ecotoxicology and Environmental Safety Syntaxin Vesicle trafficking RtSYP121 Cadmium tolerance Ion homeostasis Photosynthetic inhibition |
| title | Overexpression of RtSYP121 confers cadmium colerance by promoting vesicle trafficking, maintaining ion homeostasis, and alleviating photosynthetic inhibition in Arabidopsis |
| title_full | Overexpression of RtSYP121 confers cadmium colerance by promoting vesicle trafficking, maintaining ion homeostasis, and alleviating photosynthetic inhibition in Arabidopsis |
| title_fullStr | Overexpression of RtSYP121 confers cadmium colerance by promoting vesicle trafficking, maintaining ion homeostasis, and alleviating photosynthetic inhibition in Arabidopsis |
| title_full_unstemmed | Overexpression of RtSYP121 confers cadmium colerance by promoting vesicle trafficking, maintaining ion homeostasis, and alleviating photosynthetic inhibition in Arabidopsis |
| title_short | Overexpression of RtSYP121 confers cadmium colerance by promoting vesicle trafficking, maintaining ion homeostasis, and alleviating photosynthetic inhibition in Arabidopsis |
| title_sort | overexpression of rtsyp121 confers cadmium colerance by promoting vesicle trafficking maintaining ion homeostasis and alleviating photosynthetic inhibition in arabidopsis |
| topic | Syntaxin Vesicle trafficking RtSYP121 Cadmium tolerance Ion homeostasis Photosynthetic inhibition |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323001240 |
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