Genome-wide investigation and expression analysis of TLR gene family reveals its immune role in Vibrio tolerance challenge of Manila clam
Toll-like receptors (TLRs) play key roles in activating immune responses during infection. In this study, we identified TLR genes in Manila clam at the genome-wide level and characterized it into 9 types according to the Ruditapes philippinarum genome annotation, including TLR1 (1–10), TLR2 (1–10),...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
|
| Series: | Fish and Shellfish Immunology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667011922000226 |
| _version_ | 1811198912346718208 |
|---|---|
| author | Zhihui Yin Zihan Lin Yang Liu Xiwu Yan Hongtao Nie |
| author_facet | Zhihui Yin Zihan Lin Yang Liu Xiwu Yan Hongtao Nie |
| author_sort | Zhihui Yin |
| collection | DOAJ |
| description | Toll-like receptors (TLRs) play key roles in activating immune responses during infection. In this study, we identified TLR genes in Manila clam at the genome-wide level and characterized it into 9 types according to the Ruditapes philippinarum genome annotation, including TLR1 (1–10), TLR2 (1–10), TLR2–2 (1–5), TLR3 (1–3), TLR4 (1–9), TLR5, TLR6 (1–5), TLR7 (1–2), and TLR13 (1–4). The length of TLR proteins varied from 128 to 1257 amino acids. The molecular weights and theoretical isoelectric point (pI) values ranged from 14.63 to 143.32 kDa and 4.47 to 9.45, respectively. TLR genes showed universal expression levels in adductor muscle (AM), mantle (M), foot (F), gill (GI), pipe (P), digestive gland (DG), gonad (GO) and labial palp (L). Transcriptome analysis revealed that the expression level of TLR4, TLR5, TLR7 and TLR13 genes are significantly highly expressed in resistant individuals of Manila clam under Vibrio anguillarum challenge, indicating these TLR genes may play significant roles in response to invading pathogens. The results obtained in this work will provide valuable insights into the immune function of TLR gene in R. philippinarum. |
| first_indexed | 2024-04-12T01:39:09Z |
| format | Article |
| id | doaj.art-195a4d465c07407e9ee995130e915b9a |
| institution | Directory Open Access Journal |
| issn | 2667-0119 |
| language | English |
| last_indexed | 2024-04-12T01:39:09Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Fish and Shellfish Immunology Reports |
| spelling | doaj.art-195a4d465c07407e9ee995130e915b9a2022-12-22T03:53:14ZengElsevierFish and Shellfish Immunology Reports2667-01192022-12-013100072Genome-wide investigation and expression analysis of TLR gene family reveals its immune role in Vibrio tolerance challenge of Manila clamZhihui Yin0Zihan Lin1Yang Liu2Xiwu Yan3Hongtao Nie4College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China; Engineering and Technology Research Center of Shellfish Breeding in Liaoning Province, Dalian Ocean University, Dalian 116023, ChinaCollege of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, ChinaCollege of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, ChinaCollege of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China; Engineering and Technology Research Center of Shellfish Breeding in Liaoning Province, Dalian Ocean University, Dalian 116023, ChinaCollege of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China; Engineering and Technology Research Center of Shellfish Breeding in Liaoning Province, Dalian Ocean University, Dalian 116023, China; Corresponding author:.Toll-like receptors (TLRs) play key roles in activating immune responses during infection. In this study, we identified TLR genes in Manila clam at the genome-wide level and characterized it into 9 types according to the Ruditapes philippinarum genome annotation, including TLR1 (1–10), TLR2 (1–10), TLR2–2 (1–5), TLR3 (1–3), TLR4 (1–9), TLR5, TLR6 (1–5), TLR7 (1–2), and TLR13 (1–4). The length of TLR proteins varied from 128 to 1257 amino acids. The molecular weights and theoretical isoelectric point (pI) values ranged from 14.63 to 143.32 kDa and 4.47 to 9.45, respectively. TLR genes showed universal expression levels in adductor muscle (AM), mantle (M), foot (F), gill (GI), pipe (P), digestive gland (DG), gonad (GO) and labial palp (L). Transcriptome analysis revealed that the expression level of TLR4, TLR5, TLR7 and TLR13 genes are significantly highly expressed in resistant individuals of Manila clam under Vibrio anguillarum challenge, indicating these TLR genes may play significant roles in response to invading pathogens. The results obtained in this work will provide valuable insights into the immune function of TLR gene in R. philippinarum.http://www.sciencedirect.com/science/article/pii/S2667011922000226TLRRuditapes philippinarumImmune responseVibrio anguillarum |
| spellingShingle | Zhihui Yin Zihan Lin Yang Liu Xiwu Yan Hongtao Nie Genome-wide investigation and expression analysis of TLR gene family reveals its immune role in Vibrio tolerance challenge of Manila clam Fish and Shellfish Immunology Reports TLR Ruditapes philippinarum Immune response Vibrio anguillarum |
| title | Genome-wide investigation and expression analysis of TLR gene family reveals its immune role in Vibrio tolerance challenge of Manila clam |
| title_full | Genome-wide investigation and expression analysis of TLR gene family reveals its immune role in Vibrio tolerance challenge of Manila clam |
| title_fullStr | Genome-wide investigation and expression analysis of TLR gene family reveals its immune role in Vibrio tolerance challenge of Manila clam |
| title_full_unstemmed | Genome-wide investigation and expression analysis of TLR gene family reveals its immune role in Vibrio tolerance challenge of Manila clam |
| title_short | Genome-wide investigation and expression analysis of TLR gene family reveals its immune role in Vibrio tolerance challenge of Manila clam |
| title_sort | genome wide investigation and expression analysis of tlr gene family reveals its immune role in vibrio tolerance challenge of manila clam |
| topic | TLR Ruditapes philippinarum Immune response Vibrio anguillarum |
| url | http://www.sciencedirect.com/science/article/pii/S2667011922000226 |
| work_keys_str_mv | AT zhihuiyin genomewideinvestigationandexpressionanalysisoftlrgenefamilyrevealsitsimmuneroleinvibriotolerancechallengeofmanilaclam AT zihanlin genomewideinvestigationandexpressionanalysisoftlrgenefamilyrevealsitsimmuneroleinvibriotolerancechallengeofmanilaclam AT yangliu genomewideinvestigationandexpressionanalysisoftlrgenefamilyrevealsitsimmuneroleinvibriotolerancechallengeofmanilaclam AT xiwuyan genomewideinvestigationandexpressionanalysisoftlrgenefamilyrevealsitsimmuneroleinvibriotolerancechallengeofmanilaclam AT hongtaonie genomewideinvestigationandexpressionanalysisoftlrgenefamilyrevealsitsimmuneroleinvibriotolerancechallengeofmanilaclam |