Anaplasma phagocytophilum direct detection and exposure evidence in equines from two breeding farms from Minas Gerais State, Brazil
ABSTRACT: Equine granulocytic anaplasmosis is caused by Anaplasma phagocytophilum, a gram negative, obligatory intracellular bacterium, member of Anaplasmataceae family, included in the Rickettsiales order. Little is known about the disease, transmission dynamics, genetic diversity and prevalence in...
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| Format: | Article |
| Language: | English |
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Instituto Biológico
2018-08-01
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| Series: | Arquivos do Instituto Biológico |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100214&lng=en&tlng=en |
| _version_ | 1819027370894426112 |
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| author | Luan Gavião Prado Maristela Silveira Palhares Camila Valgas e Bastos Júlia Angélica Gonçalves da Silveira Álvaro Augusto Ramos Ribeiro Ana Luísa Soares de Miranda Vinícius Monteiro Bezerra Múcio Flávio Barbosa Ribeiro |
| author_facet | Luan Gavião Prado Maristela Silveira Palhares Camila Valgas e Bastos Júlia Angélica Gonçalves da Silveira Álvaro Augusto Ramos Ribeiro Ana Luísa Soares de Miranda Vinícius Monteiro Bezerra Múcio Flávio Barbosa Ribeiro |
| author_sort | Luan Gavião Prado |
| collection | DOAJ |
| description | ABSTRACT: Equine granulocytic anaplasmosis is caused by Anaplasma phagocytophilum, a gram negative, obligatory intracellular bacterium, member of Anaplasmataceae family, included in the Rickettsiales order. Little is known about the disease, transmission dynamics, genetic diversity and prevalence in Minas Gerais state, Brazil. This work aimed to do a serosurvey using indirect immunofluorescent assay (IFA) test and evaluation of buffy coat smears, and nested polymerase chain reaction (PCR) as diagnostic methods, to determine the disease situation in horses from two manga-larga marchador breeding farms located in the municipalities of Ataléia e São Vicente de Minas, in Minas Gerais state. It was found that 76% (131/172) of the animals were considered reactive for IFA test, and the total of 12.8% was positive at buffy coat smears analysis. At PCR analysis, it was found 1.94% of the samples positive to the infection. Those samples were sequenced and showed 96% of similarity to A. phagocytophilum from a Ixodes ricinus tick. There is a high frequency of animals with the evidence of contact to A. phagocytophilum on the two evaluated properties in this study, which was proved by positiveness in PCR analysis. New researches must be carried out to better understand the epidemiologic and clinical dynamic of the disease in the state of Minas Gerais. |
| first_indexed | 2024-12-21T05:41:24Z |
| format | Article |
| id | doaj.art-195b136e92f34111950ce7c9f570bca7 |
| institution | Directory Open Access Journal |
| issn | 1808-1657 |
| language | English |
| last_indexed | 2024-12-21T05:41:24Z |
| publishDate | 2018-08-01 |
| publisher | Instituto Biológico |
| record_format | Article |
| series | Arquivos do Instituto Biológico |
| spelling | doaj.art-195b136e92f34111950ce7c9f570bca72022-12-21T19:14:15ZengInstituto BiológicoArquivos do Instituto Biológico1808-16572018-08-0185010.1590/1808-1657000902017S1808-16572018000100214Anaplasma phagocytophilum direct detection and exposure evidence in equines from two breeding farms from Minas Gerais State, BrazilLuan Gavião PradoMaristela Silveira PalharesCamila Valgas e BastosJúlia Angélica Gonçalves da SilveiraÁlvaro Augusto Ramos RibeiroAna Luísa Soares de MirandaVinícius Monteiro BezerraMúcio Flávio Barbosa RibeiroABSTRACT: Equine granulocytic anaplasmosis is caused by Anaplasma phagocytophilum, a gram negative, obligatory intracellular bacterium, member of Anaplasmataceae family, included in the Rickettsiales order. Little is known about the disease, transmission dynamics, genetic diversity and prevalence in Minas Gerais state, Brazil. This work aimed to do a serosurvey using indirect immunofluorescent assay (IFA) test and evaluation of buffy coat smears, and nested polymerase chain reaction (PCR) as diagnostic methods, to determine the disease situation in horses from two manga-larga marchador breeding farms located in the municipalities of Ataléia e São Vicente de Minas, in Minas Gerais state. It was found that 76% (131/172) of the animals were considered reactive for IFA test, and the total of 12.8% was positive at buffy coat smears analysis. At PCR analysis, it was found 1.94% of the samples positive to the infection. Those samples were sequenced and showed 96% of similarity to A. phagocytophilum from a Ixodes ricinus tick. There is a high frequency of animals with the evidence of contact to A. phagocytophilum on the two evaluated properties in this study, which was proved by positiveness in PCR analysis. New researches must be carried out to better understand the epidemiologic and clinical dynamic of the disease in the state of Minas Gerais.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100214&lng=en&tlng=enHorsesserologyimmunofluorescencenested polymerase chain reactionAnaplasmataceae |
| spellingShingle | Luan Gavião Prado Maristela Silveira Palhares Camila Valgas e Bastos Júlia Angélica Gonçalves da Silveira Álvaro Augusto Ramos Ribeiro Ana Luísa Soares de Miranda Vinícius Monteiro Bezerra Múcio Flávio Barbosa Ribeiro Anaplasma phagocytophilum direct detection and exposure evidence in equines from two breeding farms from Minas Gerais State, Brazil Arquivos do Instituto Biológico Horses serology immunofluorescence nested polymerase chain reaction Anaplasmataceae |
| title | Anaplasma phagocytophilum direct detection and exposure evidence in equines from two breeding farms from Minas Gerais State, Brazil |
| title_full | Anaplasma phagocytophilum direct detection and exposure evidence in equines from two breeding farms from Minas Gerais State, Brazil |
| title_fullStr | Anaplasma phagocytophilum direct detection and exposure evidence in equines from two breeding farms from Minas Gerais State, Brazil |
| title_full_unstemmed | Anaplasma phagocytophilum direct detection and exposure evidence in equines from two breeding farms from Minas Gerais State, Brazil |
| title_short | Anaplasma phagocytophilum direct detection and exposure evidence in equines from two breeding farms from Minas Gerais State, Brazil |
| title_sort | anaplasma phagocytophilum direct detection and exposure evidence in equines from two breeding farms from minas gerais state brazil |
| topic | Horses serology immunofluorescence nested polymerase chain reaction Anaplasmataceae |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100214&lng=en&tlng=en |
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