First Evidence that Ecklonia cava-Derived Dieckol Attenuates MCF-7 Human Breast Carcinoma Cell Migration
We investigated the effect of Ecklonia cava (E. cava)-derived dieckol on movement behavior and the expression of migration-related genes in MCF-7 human breast cancer cell. Phlorotannins (e.g., dieckol, 6,6′-biecko, and 2,7″-phloroglucinol-6,6′-bieckol) were purified from E. cava by using centrifugal...
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MDPI AG
2015-03-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/13/4/1785 |
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| author | Eun-Kyung Kim Yujiao Tang Yon-Suk Kim Jin-Woo Hwang Eun-Ju Choi Ji-Hyeok Lee Seung-Hong Lee You-Jin Jeon Pyo-Jam Park |
| author_facet | Eun-Kyung Kim Yujiao Tang Yon-Suk Kim Jin-Woo Hwang Eun-Ju Choi Ji-Hyeok Lee Seung-Hong Lee You-Jin Jeon Pyo-Jam Park |
| author_sort | Eun-Kyung Kim |
| collection | DOAJ |
| description | We investigated the effect of Ecklonia cava (E. cava)-derived dieckol on movement behavior and the expression of migration-related genes in MCF-7 human breast cancer cell. Phlorotannins (e.g., dieckol, 6,6′-biecko, and 2,7″-phloroglucinol-6,6′-bieckol) were purified from E. cava by using centrifugal partition chromatography. Among the phlorotannins, we found that dieckol inhibited breast cancer cell the most and was selected for further study. Radius™-well was used to assess cell migration, and dieckol (1–100 µM) was found to suppress breast cancer cell movement. Metastasis-related gene expressions were evaluated by RT-PCR and Western blot analysis. In addition, dieckol inhibited the expression of migration-related genes such as matrix metalloproteinase (MMP)-9 and vascular endothelial growth factor (VEGF). On the other hand, it stimulated the expression of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2. These results suggest that dieckol exerts anti-breast cancer activity via the regulation of the expressions of metastasis-related genes, and this is the first report on the anti-breast cancer effect of dieckol. |
| first_indexed | 2024-04-11T20:56:12Z |
| format | Article |
| id | doaj.art-195deb9835d54d4da2c2724525932301 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T20:56:12Z |
| publishDate | 2015-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-195deb9835d54d4da2c27245259323012022-12-22T04:03:40ZengMDPI AGMarine Drugs1660-33972015-03-011341785179710.3390/md13041785md13041785First Evidence that Ecklonia cava-Derived Dieckol Attenuates MCF-7 Human Breast Carcinoma Cell MigrationEun-Kyung Kim0Yujiao Tang1Yon-Suk Kim2Jin-Woo Hwang3Eun-Ju Choi4Ji-Hyeok Lee5Seung-Hong Lee6You-Jin Jeon7Pyo-Jam Park8Division of Food Bio Science, College of Biomedical and Health Sciences, Konkuk University, Chungju 380-701, KoreaDivision of Food Bio Science, College of Biomedical and Health Sciences, Konkuk University, Chungju 380-701, KoreaKorea Nokyong Research Center, Konkuk University, Chungju 380-701, KoreaKorea Nokyong Research Center, Konkuk University, Chungju 380-701, KoreaDivision of Sport Science, Konkuk University, Chungju, 380-701, KoreaDepartment of Marine Life Science, Jeju National University, Jeju 690-756, KoreaDivision of Food Bio Science, College of Biomedical and Health Sciences, Konkuk University, Chungju 380-701, KoreaDepartment of Marine Life Science, Jeju National University, Jeju 690-756, KoreaKorea Nokyong Research Center, Konkuk University, Chungju 380-701, KoreaWe investigated the effect of Ecklonia cava (E. cava)-derived dieckol on movement behavior and the expression of migration-related genes in MCF-7 human breast cancer cell. Phlorotannins (e.g., dieckol, 6,6′-biecko, and 2,7″-phloroglucinol-6,6′-bieckol) were purified from E. cava by using centrifugal partition chromatography. Among the phlorotannins, we found that dieckol inhibited breast cancer cell the most and was selected for further study. Radius™-well was used to assess cell migration, and dieckol (1–100 µM) was found to suppress breast cancer cell movement. Metastasis-related gene expressions were evaluated by RT-PCR and Western blot analysis. In addition, dieckol inhibited the expression of migration-related genes such as matrix metalloproteinase (MMP)-9 and vascular endothelial growth factor (VEGF). On the other hand, it stimulated the expression of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2. These results suggest that dieckol exerts anti-breast cancer activity via the regulation of the expressions of metastasis-related genes, and this is the first report on the anti-breast cancer effect of dieckol.http://www.mdpi.com/1660-3397/13/4/1785Ecklonia cavaphlorotanninsdieckolbreast cancermigration |
| spellingShingle | Eun-Kyung Kim Yujiao Tang Yon-Suk Kim Jin-Woo Hwang Eun-Ju Choi Ji-Hyeok Lee Seung-Hong Lee You-Jin Jeon Pyo-Jam Park First Evidence that Ecklonia cava-Derived Dieckol Attenuates MCF-7 Human Breast Carcinoma Cell Migration Marine Drugs Ecklonia cava phlorotannins dieckol breast cancer migration |
| title | First Evidence that Ecklonia cava-Derived Dieckol Attenuates MCF-7 Human Breast Carcinoma Cell Migration |
| title_full | First Evidence that Ecklonia cava-Derived Dieckol Attenuates MCF-7 Human Breast Carcinoma Cell Migration |
| title_fullStr | First Evidence that Ecklonia cava-Derived Dieckol Attenuates MCF-7 Human Breast Carcinoma Cell Migration |
| title_full_unstemmed | First Evidence that Ecklonia cava-Derived Dieckol Attenuates MCF-7 Human Breast Carcinoma Cell Migration |
| title_short | First Evidence that Ecklonia cava-Derived Dieckol Attenuates MCF-7 Human Breast Carcinoma Cell Migration |
| title_sort | first evidence that ecklonia cava derived dieckol attenuates mcf 7 human breast carcinoma cell migration |
| topic | Ecklonia cava phlorotannins dieckol breast cancer migration |
| url | http://www.mdpi.com/1660-3397/13/4/1785 |
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