A study of Bcl-2 and p-53 immunostaining expressions in colonic carcinomas
Background: Colorectal cancer burden has been steadily rising in women. It was the fourth most common cancer in 1975 and has reached the second position by 1990, with about 49% increase in the number of cases globally over a period of 15 yrs. From 1999–2008, the rate of people dying from colorectal...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | MRIMS Journal of Health Sciences |
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| Online Access: | http://www.mrimsjournal.com/article.asp?issn=2321-7006;year=2016;volume=4;issue=1;spage=28;epage=32;aulast=Kiran;type=0 |
| _version_ | 1828789035221385216 |
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| author | K Shashi Kiran V Hari Shanker Triveni Bhopal Kandukuri Mahesh Kumar |
| author_facet | K Shashi Kiran V Hari Shanker Triveni Bhopal Kandukuri Mahesh Kumar |
| author_sort | K Shashi Kiran |
| collection | DOAJ |
| description | Background: Colorectal cancer burden has been steadily rising in women. It was the fourth most common cancer in 1975 and has reached the second position by 1990, with about 49% increase in the number of cases globally over a period of 15 yrs. From 1999–2008, the rate of people dying from colorectal cancer has varied, depending on their race and ethnicity.
Objective: To study Bcl-2 and p-53 immunostaining expressions in colonic carcinomas.
Methods: The present study is both a retrospective and a prospective study. The study is based on histomorphological evaluation of 30 resected specimen of Colorectal Carcinomas received at the department of Pathology, Osmania General Hospital in a 2 year period from June 2009 to May 2011. The most representative block for all 30 cases was then selected for immune histochemical analysis of p-53 protein and Bcl-2 Oncoprotein for p-53 immunostaining, we used the DO-7 monoclonal antibody (dilution 1:30; DAKO), and, for Bcl-2, the monoclonal antibody 124 was used (dilution 1:80; DAKO).
Results: Positive staining for p-53 protein was detected in 56.67% ( 17/30 ) of colorectal adenocarcinomas. There is statistically no significant association observed between p53 expression and Dukes' stage, TNM stage or Histological grade (p value > 0.05). Similarly no association was found between expression of p-53 and other patient variables, such as age, sex, location of tumor, & tumor differentiation. In the present study statistically there is no significant association between Bcl-2 & p-53 either individually or combined together with any of the parameters (like age, sex, tumor location, Dukes' stage, Histological grade & TNM stage).
Conclusion: Though not statistically significant, to comment as the optimism expressed by certain investigators due to the low number of cases taken up for the present study, a large study group is needed to comment on the prognostic significance of the Bcl-2 & p-53 immuno profile individually and in a combined variation to predict the clinical outcome. |
| first_indexed | 2024-12-12T01:04:06Z |
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| id | doaj.art-195f9803d19244669bb4c7f60cc18d1c |
| institution | Directory Open Access Journal |
| issn | 2321-7006 2321-7294 |
| language | English |
| last_indexed | 2024-12-12T01:04:06Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
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| series | MRIMS Journal of Health Sciences |
| spelling | doaj.art-195f9803d19244669bb4c7f60cc18d1c2022-12-22T00:43:37ZengWolters Kluwer Medknow PublicationsMRIMS Journal of Health Sciences2321-70062321-72942016-01-0141283210.4103/2321-7006.303061A study of Bcl-2 and p-53 immunostaining expressions in colonic carcinomasK Shashi KiranV Hari ShankerTriveni BhopalKandukuri Mahesh KumarBackground: Colorectal cancer burden has been steadily rising in women. It was the fourth most common cancer in 1975 and has reached the second position by 1990, with about 49% increase in the number of cases globally over a period of 15 yrs. From 1999–2008, the rate of people dying from colorectal cancer has varied, depending on their race and ethnicity. Objective: To study Bcl-2 and p-53 immunostaining expressions in colonic carcinomas. Methods: The present study is both a retrospective and a prospective study. The study is based on histomorphological evaluation of 30 resected specimen of Colorectal Carcinomas received at the department of Pathology, Osmania General Hospital in a 2 year period from June 2009 to May 2011. The most representative block for all 30 cases was then selected for immune histochemical analysis of p-53 protein and Bcl-2 Oncoprotein for p-53 immunostaining, we used the DO-7 monoclonal antibody (dilution 1:30; DAKO), and, for Bcl-2, the monoclonal antibody 124 was used (dilution 1:80; DAKO). Results: Positive staining for p-53 protein was detected in 56.67% ( 17/30 ) of colorectal adenocarcinomas. There is statistically no significant association observed between p53 expression and Dukes' stage, TNM stage or Histological grade (p value > 0.05). Similarly no association was found between expression of p-53 and other patient variables, such as age, sex, location of tumor, & tumor differentiation. In the present study statistically there is no significant association between Bcl-2 & p-53 either individually or combined together with any of the parameters (like age, sex, tumor location, Dukes' stage, Histological grade & TNM stage). Conclusion: Though not statistically significant, to comment as the optimism expressed by certain investigators due to the low number of cases taken up for the present study, a large study group is needed to comment on the prognostic significance of the Bcl-2 & p-53 immuno profile individually and in a combined variation to predict the clinical outcome.http://www.mrimsjournal.com/article.asp?issn=2321-7006;year=2016;volume=4;issue=1;spage=28;epage=32;aulast=Kiran;type=0bcl-2p-53immunostaining |
| spellingShingle | K Shashi Kiran V Hari Shanker Triveni Bhopal Kandukuri Mahesh Kumar A study of Bcl-2 and p-53 immunostaining expressions in colonic carcinomas MRIMS Journal of Health Sciences bcl-2 p-53 immunostaining |
| title | A study of Bcl-2 and p-53 immunostaining expressions in colonic carcinomas |
| title_full | A study of Bcl-2 and p-53 immunostaining expressions in colonic carcinomas |
| title_fullStr | A study of Bcl-2 and p-53 immunostaining expressions in colonic carcinomas |
| title_full_unstemmed | A study of Bcl-2 and p-53 immunostaining expressions in colonic carcinomas |
| title_short | A study of Bcl-2 and p-53 immunostaining expressions in colonic carcinomas |
| title_sort | study of bcl 2 and p 53 immunostaining expressions in colonic carcinomas |
| topic | bcl-2 p-53 immunostaining |
| url | http://www.mrimsjournal.com/article.asp?issn=2321-7006;year=2016;volume=4;issue=1;spage=28;epage=32;aulast=Kiran;type=0 |
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