Lotus (Nelumbo nucifera Gaertn.) leaf water extracts suppress influenza a viral infection via inhibition of neuraminidase and hemagglutinin
Lotus (Nelumbo nucifera Gaertn.) is a perennial aquatic plant, that has been used in folk to treat various diseases. Here, we first demonstrate the water extract of lotus leaf (WLL) has a strong inhibitory effect on influenza A virus (IAV) infection by inhibiting neuraminidase (NA) and hemagglutinin...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622000895 |
| _version_ | 1818773084971204608 |
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| author | Won-Kyung Cho Hye Jin Yang Jin Yeul Ma |
| author_facet | Won-Kyung Cho Hye Jin Yang Jin Yeul Ma |
| author_sort | Won-Kyung Cho |
| collection | DOAJ |
| description | Lotus (Nelumbo nucifera Gaertn.) is a perennial aquatic plant, that has been used in folk to treat various diseases. Here, we first demonstrate the water extract of lotus leaf (WLL) has a strong inhibitory effect on influenza A virus (IAV) infection by inhibiting neuraminidase (NA) and hemagglutinin (HA). To investigate the effect of WLL on IAV infection, we used green fluorescent protein (GFP)-tagged Influenza A/PR/8/34, fluorescence-activated cell sorting (FACS) analysis, and plaque reduction assay. WLL significantly repressed viral infection, dose-dependently. Immunofluorescence (IF) analysis confirmed that WLL reduces influenza HA, NA, M2, and NP viral proteins expression. WLL strongly inhibited HA and reduced NA activity of IAV. Among six components in WLL, isoquercitrin exerted potent antiviral efficacy. Additionally, WLL potently inhibited the cytopathic effect caused by H1N1 or H3N2 IAV. In conclusion, our results suggest that WLL could be used as a natural viral inhibitor for H1N1 and H3N2 influenza viral infection. |
| first_indexed | 2024-12-18T10:19:38Z |
| format | Article |
| id | doaj.art-196322d1710f4e67aefad2643281224d |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-18T10:19:38Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-196322d1710f4e67aefad2643281224d2022-12-21T21:11:10ZengElsevierJournal of Functional Foods1756-46462022-04-0191105019Lotus (Nelumbo nucifera Gaertn.) leaf water extracts suppress influenza a viral infection via inhibition of neuraminidase and hemagglutininWon-Kyung Cho0Hye Jin Yang1Jin Yeul Ma2Corresponding author.; KM Application Center, Korea Institute of Oriental Medicine, 70 chemdanro, Dong-gu, Daegu 41062, Republic of KoreaKM Application Center, Korea Institute of Oriental Medicine, 70 chemdanro, Dong-gu, Daegu 41062, Republic of KoreaCorresponding author.; KM Application Center, Korea Institute of Oriental Medicine, 70 chemdanro, Dong-gu, Daegu 41062, Republic of KoreaLotus (Nelumbo nucifera Gaertn.) is a perennial aquatic plant, that has been used in folk to treat various diseases. Here, we first demonstrate the water extract of lotus leaf (WLL) has a strong inhibitory effect on influenza A virus (IAV) infection by inhibiting neuraminidase (NA) and hemagglutinin (HA). To investigate the effect of WLL on IAV infection, we used green fluorescent protein (GFP)-tagged Influenza A/PR/8/34, fluorescence-activated cell sorting (FACS) analysis, and plaque reduction assay. WLL significantly repressed viral infection, dose-dependently. Immunofluorescence (IF) analysis confirmed that WLL reduces influenza HA, NA, M2, and NP viral proteins expression. WLL strongly inhibited HA and reduced NA activity of IAV. Among six components in WLL, isoquercitrin exerted potent antiviral efficacy. Additionally, WLL potently inhibited the cytopathic effect caused by H1N1 or H3N2 IAV. In conclusion, our results suggest that WLL could be used as a natural viral inhibitor for H1N1 and H3N2 influenza viral infection.http://www.sciencedirect.com/science/article/pii/S1756464622000895Nelumbo nucifera Gaertn. (Nymphaeaceae) LeafInfluenza A virusCytopathic effectViral inhibitorIsoquercitrin |
| spellingShingle | Won-Kyung Cho Hye Jin Yang Jin Yeul Ma Lotus (Nelumbo nucifera Gaertn.) leaf water extracts suppress influenza a viral infection via inhibition of neuraminidase and hemagglutinin Journal of Functional Foods Nelumbo nucifera Gaertn. (Nymphaeaceae) Leaf Influenza A virus Cytopathic effect Viral inhibitor Isoquercitrin |
| title | Lotus (Nelumbo nucifera Gaertn.) leaf water extracts suppress influenza a viral infection via inhibition of neuraminidase and hemagglutinin |
| title_full | Lotus (Nelumbo nucifera Gaertn.) leaf water extracts suppress influenza a viral infection via inhibition of neuraminidase and hemagglutinin |
| title_fullStr | Lotus (Nelumbo nucifera Gaertn.) leaf water extracts suppress influenza a viral infection via inhibition of neuraminidase and hemagglutinin |
| title_full_unstemmed | Lotus (Nelumbo nucifera Gaertn.) leaf water extracts suppress influenza a viral infection via inhibition of neuraminidase and hemagglutinin |
| title_short | Lotus (Nelumbo nucifera Gaertn.) leaf water extracts suppress influenza a viral infection via inhibition of neuraminidase and hemagglutinin |
| title_sort | lotus nelumbo nucifera gaertn leaf water extracts suppress influenza a viral infection via inhibition of neuraminidase and hemagglutinin |
| topic | Nelumbo nucifera Gaertn. (Nymphaeaceae) Leaf Influenza A virus Cytopathic effect Viral inhibitor Isoquercitrin |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622000895 |
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