Surface Characteristics and Microbiological Analysis of a Vat-Photopolymerization Additive-Manufacturing Dental Resin
The wide application of additive manufacturing in dentistry implies the further investigation into oral micro-organism adhesion and biofilm formation on vat-photopolymerization (VP) dental resins. The surface characteristics and microbiological analysis of a VP dental resin, printed at resolutions o...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/1996-1944/15/2/425 |
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| author | Ericles Otávio Santos Pedro Lima Emmerich Oliveira Thaís Pereira de Mello André Luis Souza dos Santos Carlos Nelson Elias Sung-Hwan Choi Amanda Cunha Regal de Castro |
| author_facet | Ericles Otávio Santos Pedro Lima Emmerich Oliveira Thaís Pereira de Mello André Luis Souza dos Santos Carlos Nelson Elias Sung-Hwan Choi Amanda Cunha Regal de Castro |
| author_sort | Ericles Otávio Santos |
| collection | DOAJ |
| description | The wide application of additive manufacturing in dentistry implies the further investigation into oral micro-organism adhesion and biofilm formation on vat-photopolymerization (VP) dental resins. The surface characteristics and microbiological analysis of a VP dental resin, printed at resolutions of 50 μm (EG-50) and 100 μm (EG-100), were evaluated against an auto-polymerizing acrylic resin (CG). Samples were evaluated using a scanning electron microscope, a scanning white-light interferometer, and analyzed for <i>Candida albicans (CA)</i> and <i>Streptococcus mutans (SM)</i> biofilm, as well as antifungal and antimicrobial activity. EG-50 and EG-100 exhibited more irregular surfaces and statistically higher mean (Ra) and root-mean-square (rms) roughness (EG-50-Ra: 2.96 ± 0.32 µm; rms: 4.05 ± 0.43 µm/EG-100-Ra: 3.76 ± 0.58 µm; rms: 4.79 ± 0.74 µm) compared to the CG (Ra: 0.52 ± 0.36 µm; rms: 0.84 ± 0.54 µm) (<i>p</i> < 0.05). The biomass and extracellular matrix production by <i>CA</i> and <i>SM</i> and the metabolic activity of <i>SM</i> were significantly decreased in EG-50 and EG-100 compared to CG (<i>p</i> < 0.05). <i>CA</i> and <i>SM</i> growth was inhibited by the pure unpolymerized VP resin (48 h). EG-50 and EG-100 recorded a greater irregularity, higher surface roughness, and decreased <i>CA</i> and <i>SM</i> biofilm formation over the CG. |
| first_indexed | 2024-03-10T01:04:13Z |
| format | Article |
| id | doaj.art-1966c84dee104195ab862ea807ab6f72 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T01:04:13Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-1966c84dee104195ab862ea807ab6f722023-11-23T14:28:54ZengMDPI AGMaterials1996-19442022-01-0115242510.3390/ma15020425Surface Characteristics and Microbiological Analysis of a Vat-Photopolymerization Additive-Manufacturing Dental ResinEricles Otávio Santos0Pedro Lima Emmerich Oliveira1Thaís Pereira de Mello2André Luis Souza dos Santos3Carlos Nelson Elias4Sung-Hwan Choi5Amanda Cunha Regal de Castro6Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941617, RJ, BrazilDepartment of Pediatric Dentistry and Orthodontics, School of Dentistry, Escola Superior São Francisco de Assis, Santa Teresa 29650000, ES, BrazilLaboratory for Advanced Studies of Emerging and Resistant Microorganisms, Department of General Microbiology, Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro 21941902, RJ, BrazilLaboratory for Advanced Studies of Emerging and Resistant Microorganisms, Department of General Microbiology, Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro 21941902, RJ, BrazilDepartment of Mechanical Engineering and Materials Science, Military Institute of Engineering, Rio de Janeiro 22290270, RJ, BrazilDepartment of Orthodontics, Institute of Craniofacial Deformity, Yonsei University College of Dentistry, Seoul 03772, KoreaDepartment of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941617, RJ, BrazilThe wide application of additive manufacturing in dentistry implies the further investigation into oral micro-organism adhesion and biofilm formation on vat-photopolymerization (VP) dental resins. The surface characteristics and microbiological analysis of a VP dental resin, printed at resolutions of 50 μm (EG-50) and 100 μm (EG-100), were evaluated against an auto-polymerizing acrylic resin (CG). Samples were evaluated using a scanning electron microscope, a scanning white-light interferometer, and analyzed for <i>Candida albicans (CA)</i> and <i>Streptococcus mutans (SM)</i> biofilm, as well as antifungal and antimicrobial activity. EG-50 and EG-100 exhibited more irregular surfaces and statistically higher mean (Ra) and root-mean-square (rms) roughness (EG-50-Ra: 2.96 ± 0.32 µm; rms: 4.05 ± 0.43 µm/EG-100-Ra: 3.76 ± 0.58 µm; rms: 4.79 ± 0.74 µm) compared to the CG (Ra: 0.52 ± 0.36 µm; rms: 0.84 ± 0.54 µm) (<i>p</i> < 0.05). The biomass and extracellular matrix production by <i>CA</i> and <i>SM</i> and the metabolic activity of <i>SM</i> were significantly decreased in EG-50 and EG-100 compared to CG (<i>p</i> < 0.05). <i>CA</i> and <i>SM</i> growth was inhibited by the pure unpolymerized VP resin (48 h). EG-50 and EG-100 recorded a greater irregularity, higher surface roughness, and decreased <i>CA</i> and <i>SM</i> biofilm formation over the CG.https://www.mdpi.com/1996-1944/15/2/425digital technologiesbiomaterialspoly methyl methacrylate resinphoto-polymerizable resinsurface propertiesantifungal activity |
| spellingShingle | Ericles Otávio Santos Pedro Lima Emmerich Oliveira Thaís Pereira de Mello André Luis Souza dos Santos Carlos Nelson Elias Sung-Hwan Choi Amanda Cunha Regal de Castro Surface Characteristics and Microbiological Analysis of a Vat-Photopolymerization Additive-Manufacturing Dental Resin Materials digital technologies biomaterials poly methyl methacrylate resin photo-polymerizable resin surface properties antifungal activity |
| title | Surface Characteristics and Microbiological Analysis of a Vat-Photopolymerization Additive-Manufacturing Dental Resin |
| title_full | Surface Characteristics and Microbiological Analysis of a Vat-Photopolymerization Additive-Manufacturing Dental Resin |
| title_fullStr | Surface Characteristics and Microbiological Analysis of a Vat-Photopolymerization Additive-Manufacturing Dental Resin |
| title_full_unstemmed | Surface Characteristics and Microbiological Analysis of a Vat-Photopolymerization Additive-Manufacturing Dental Resin |
| title_short | Surface Characteristics and Microbiological Analysis of a Vat-Photopolymerization Additive-Manufacturing Dental Resin |
| title_sort | surface characteristics and microbiological analysis of a vat photopolymerization additive manufacturing dental resin |
| topic | digital technologies biomaterials poly methyl methacrylate resin photo-polymerizable resin surface properties antifungal activity |
| url | https://www.mdpi.com/1996-1944/15/2/425 |
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