Genosensing on a 3D-printed nanocarbon electrode
In this paper we present the characterization of 3D-printed nanocarbon electrodes (3DnCes) and their application in electrochemical enzyme-linked detection of DNA hybridization. The approach takes advantage of a facile procedure based on adsorption of target DNA on the electrode surface followed by...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Electrochemistry Communications |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1388248123000826 |
| _version_ | 1797811040049692672 |
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| author | Jyoti Miroslav Fojta Monika Hermanová Hana Pivoňková Osamah Alduhaish Martin Pumera |
| author_facet | Jyoti Miroslav Fojta Monika Hermanová Hana Pivoňková Osamah Alduhaish Martin Pumera |
| author_sort | Jyoti |
| collection | DOAJ |
| description | In this paper we present the characterization of 3D-printed nanocarbon electrodes (3DnCes) and their application in electrochemical enzyme-linked detection of DNA hybridization. The approach takes advantage of a facile procedure based on adsorption of target DNA on the electrode surface followed by hybridization with a biotinylated probe and binding of streptavidin–alkaline phosphatase conjugate. The alkaline phosphatase converts 1-naphthyl phosphate in the background electrolyte into electrochemically oxidizable 1-naphthol, which is subsequently detected using linear sweep voltammetry. The preparation, characterization, and analytical performance of the 3DnCes are reported. The results show the applicability of such 3DnCes in detection of target DNA hybridization specifically with the complementary biotinylated probe, and indicate the potential of 3D printed electrodes for use in various bioanalytical approaches. |
| first_indexed | 2024-03-13T07:17:45Z |
| format | Article |
| id | doaj.art-1969f4a345bc40f3b396c45471313f0c |
| institution | Directory Open Access Journal |
| issn | 1388-2481 |
| language | English |
| last_indexed | 2024-03-13T07:17:45Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electrochemistry Communications |
| spelling | doaj.art-1969f4a345bc40f3b396c45471313f0c2023-06-05T04:12:37ZengElsevierElectrochemistry Communications1388-24812023-06-01151107508Genosensing on a 3D-printed nanocarbon electrode Jyoti0Miroslav Fojta1Monika Hermanová2Hana Pivoňková3Osamah Alduhaish4Martin Pumera5Future Energy and Innovation Laboratory, Central European Institute of Technology, Brno University of Technology, Purkyňova, 123, CZ-612 00 Brno, Czech RepublicInstitute of Biophysics, Czech Academy of Sciences, Královopolská 135, CZ-612 65 Brno, Czech RepublicInstitute of Biophysics, Czech Academy of Sciences, Královopolská 135, CZ-612 65 Brno, Czech RepublicInstitute of Biophysics, Czech Academy of Sciences, Královopolská 135, CZ-612 65 Brno, Czech RepublicChemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaFuture Energy and Innovation Laboratory, Central European Institute of Technology, Brno University of Technology, Purkyňova, 123, CZ-612 00 Brno, Czech Republic; Chemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; Faculty of Electrical Engineering and Computer Science, VSB - Technical University of Ostrava, 17, Listopadu 2172/15, 70800 Ostrava, Czech Republic; Department of Medical Research, China Medical University Hospital, China Medical University, No. 91, Hsueh-Shih Road, 40402 Taichung, Taiwan; Corresponding author at: Future Energy and Innovation Laboratory, Central European Institute of Technology, Brno University of Technology, Purkyňova, 123, CZ-612 00 Brno, Czech Republic.In this paper we present the characterization of 3D-printed nanocarbon electrodes (3DnCes) and their application in electrochemical enzyme-linked detection of DNA hybridization. The approach takes advantage of a facile procedure based on adsorption of target DNA on the electrode surface followed by hybridization with a biotinylated probe and binding of streptavidin–alkaline phosphatase conjugate. The alkaline phosphatase converts 1-naphthyl phosphate in the background electrolyte into electrochemically oxidizable 1-naphthol, which is subsequently detected using linear sweep voltammetry. The preparation, characterization, and analytical performance of the 3DnCes are reported. The results show the applicability of such 3DnCes in detection of target DNA hybridization specifically with the complementary biotinylated probe, and indicate the potential of 3D printed electrodes for use in various bioanalytical approaches.http://www.sciencedirect.com/science/article/pii/S13882481230008263D-printed nanocarbon electrodeDNA hybridizationElectrochemical analysisAdditive manufacturing |
| spellingShingle | Jyoti Miroslav Fojta Monika Hermanová Hana Pivoňková Osamah Alduhaish Martin Pumera Genosensing on a 3D-printed nanocarbon electrode Electrochemistry Communications 3D-printed nanocarbon electrode DNA hybridization Electrochemical analysis Additive manufacturing |
| title | Genosensing on a 3D-printed nanocarbon electrode |
| title_full | Genosensing on a 3D-printed nanocarbon electrode |
| title_fullStr | Genosensing on a 3D-printed nanocarbon electrode |
| title_full_unstemmed | Genosensing on a 3D-printed nanocarbon electrode |
| title_short | Genosensing on a 3D-printed nanocarbon electrode |
| title_sort | genosensing on a 3d printed nanocarbon electrode |
| topic | 3D-printed nanocarbon electrode DNA hybridization Electrochemical analysis Additive manufacturing |
| url | http://www.sciencedirect.com/science/article/pii/S1388248123000826 |
| work_keys_str_mv | AT jyoti genosensingona3dprintednanocarbonelectrode AT miroslavfojta genosensingona3dprintednanocarbonelectrode AT monikahermanova genosensingona3dprintednanocarbonelectrode AT hanapivonkova genosensingona3dprintednanocarbonelectrode AT osamahalduhaish genosensingona3dprintednanocarbonelectrode AT martinpumera genosensingona3dprintednanocarbonelectrode |