Environmentally relevant concentrations of arsenic induces apoptosis in the early life stage of zebrafish
Arsenic (As) in the aquatic environment is a considerable environmental issue, previous studies have reported the toxic effects of low concentrations (≤ 150 μg/L) of As on fish. However, limited information is available regarding the impact of low levels of As on apoptosis. To evaluate this, zebrafi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-12-01
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| Series: | Ecotoxicology and Environmental Safety |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651321009957 |
| _version_ | 1818420763253800960 |
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| author | Wen-Jun Zhao Xiang Li Ze-Qiong Xu Ke-Ming Fang Hua-Chang Hong Hong-Jie Sun Dong-Xing Guan Xin-Wei Yu |
| author_facet | Wen-Jun Zhao Xiang Li Ze-Qiong Xu Ke-Ming Fang Hua-Chang Hong Hong-Jie Sun Dong-Xing Guan Xin-Wei Yu |
| author_sort | Wen-Jun Zhao |
| collection | DOAJ |
| description | Arsenic (As) in the aquatic environment is a considerable environmental issue, previous studies have reported the toxic effects of low concentrations (≤ 150 μg/L) of As on fish. However, limited information is available regarding the impact of low levels of As on apoptosis. To evaluate this, zebrafish embryos were exposed to different concentrations (0, 25, 50, 75, and 150 μg/L) of As (arsenite [AsIII] and arsenate [AsV]) for 120 h. Our results indicated that low concentrations of AsIII exposure significantly inhibited the survival of zebrafish larvae, and significantly increased the transcription of Caspase-9 and Caspase-3, the ratio of Bax/Bcl-2 transcription, and protein levels of Caspase-3. In contrast, AsV decreased the ratios of Bax/Bcl-2 transcription and protein levels, as well as protein levels of Caspase-3. Our data demonstrated that AsIII and AsV exert different toxic effects, AsIII induced apoptosis via the mitochondrial pathway and the extrinsic pathway, while AsV induced apoptosis only via the mitochondrial pathway. |
| first_indexed | 2024-12-14T12:59:38Z |
| format | Article |
| id | doaj.art-1970bb89868f46eea44eb442af952083 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-14T12:59:38Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-1970bb89868f46eea44eb442af9520832022-12-21T23:00:28ZengElsevierEcotoxicology and Environmental Safety0147-65132021-12-01227112883Environmentally relevant concentrations of arsenic induces apoptosis in the early life stage of zebrafishWen-Jun Zhao0Xiang Li1Ze-Qiong Xu2Ke-Ming Fang3Hua-Chang Hong4Hong-Jie Sun5Dong-Xing Guan6Xin-Wei Yu7College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, ChinaCollege of Geography and Environmental Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, ChinaCollege of Geography and Environmental Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, ChinaCollege of Geography and Environmental Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, ChinaCollege of Geography and Environmental Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, ChinaCollege of Geography and Environmental Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China; Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Science, Zhejiang University, Hangzhou, Zhejiang 310058, China; Corresponding author at: College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China.Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Science, Zhejiang University, Hangzhou, Zhejiang 310058, ChinaKey Laboratory of Health Risk Factors for Seafood of Zhejiang Province, Zhoushan Municipal Center for Disease Control and Prevention, Zhoushan, Zhejiang 316021, China; College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316021, China; Corresponding author.Arsenic (As) in the aquatic environment is a considerable environmental issue, previous studies have reported the toxic effects of low concentrations (≤ 150 μg/L) of As on fish. However, limited information is available regarding the impact of low levels of As on apoptosis. To evaluate this, zebrafish embryos were exposed to different concentrations (0, 25, 50, 75, and 150 μg/L) of As (arsenite [AsIII] and arsenate [AsV]) for 120 h. Our results indicated that low concentrations of AsIII exposure significantly inhibited the survival of zebrafish larvae, and significantly increased the transcription of Caspase-9 and Caspase-3, the ratio of Bax/Bcl-2 transcription, and protein levels of Caspase-3. In contrast, AsV decreased the ratios of Bax/Bcl-2 transcription and protein levels, as well as protein levels of Caspase-3. Our data demonstrated that AsIII and AsV exert different toxic effects, AsIII induced apoptosis via the mitochondrial pathway and the extrinsic pathway, while AsV induced apoptosis only via the mitochondrial pathway.http://www.sciencedirect.com/science/article/pii/S0147651321009957 |
| spellingShingle | Wen-Jun Zhao Xiang Li Ze-Qiong Xu Ke-Ming Fang Hua-Chang Hong Hong-Jie Sun Dong-Xing Guan Xin-Wei Yu Environmentally relevant concentrations of arsenic induces apoptosis in the early life stage of zebrafish Ecotoxicology and Environmental Safety |
| title | Environmentally relevant concentrations of arsenic induces apoptosis in the early life stage of zebrafish |
| title_full | Environmentally relevant concentrations of arsenic induces apoptosis in the early life stage of zebrafish |
| title_fullStr | Environmentally relevant concentrations of arsenic induces apoptosis in the early life stage of zebrafish |
| title_full_unstemmed | Environmentally relevant concentrations of arsenic induces apoptosis in the early life stage of zebrafish |
| title_short | Environmentally relevant concentrations of arsenic induces apoptosis in the early life stage of zebrafish |
| title_sort | environmentally relevant concentrations of arsenic induces apoptosis in the early life stage of zebrafish |
| url | http://www.sciencedirect.com/science/article/pii/S0147651321009957 |
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