| Summary: | Novel composites of Ag and ZnO nanoparticles with prepared transition metal complexes were obtained. The transition metal complexes are obtained by the interaction of the ligand N,N′-bis(salicylaldehyde)-o-phenylenediamine(H2B) with some transition metal ions (Co(II), Ni(II), Cu(II) and Zn(II)). The structures of the ligand and the formed complexes were characterized by elemental analysis, FTIR, UV–Vis, 1H NMR, mass spectra, thermal (TG/DTA), magnetic and conductivity measurements. The obtained complexes have the molecular formula [MB(H2O)x]. yH2O. The found conductivity revealed the non-electrolytic behavior of the formed complexes. The magnetic susceptibility and UV–Vis spectra indicated the octahedral structure of the formed complexes. The thermodynamic and kinetic parameters are estimated for the formed complexes. The interaction of the formed compounds with the FM-DNA was tested. The evaluation of the binding constant (Kb) using Bensi-Hildebrand plot, revealed the moderate interaction with electrostatic behavior. Silver and zinc oxide nanoparticles were prepared and characterized by XRD, UV–Vis, FTIR spectra and TEM analysis. The biological activity of the synthesized composites of Ag and ZnO nanoparticles with the prepared transition metal complexes were tested against some Gram positive and Gram negative bacteria as well as Fungi. The obtained inhibition zone supported the enhancement in the activity of the prepared compounds towards the target microorganism after forming the composites. Also, the interaction with the E. coli protein (FabH, PDB code: 1HNJ) was tested and evaluated using the docking operation with aid of some computational chemistry software. The obtained results agreed well with our experimental work. Keywords: DNA, Nanoparticles, Biological activity, Docking, Complexes, Composites
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