Addressing widespread misidentifications of traditional medicinal mushrooms in Sanghuangporus (Basidiomycota) through ITS barcoding and designation of reference sequences
Abstract “Sanghuang” refers to a group of important traditionally-used medicinal mushrooms belonging to the genus Sanghuangporus. In practice, species of Sanghuangporus referred to in medicinal studies and industry are now differentiated mainly by a BLAST search of GenBank with the ITS barcoding reg...
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| Format: | Article |
| Language: | English |
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BMC
2021-04-01
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| Series: | IMA Fungus |
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| Online Access: | https://doi.org/10.1186/s43008-021-00059-x |
| _version_ | 1818728956857155584 |
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| author | Shan Shen Shi-Liang Liu Ji-Hang Jiang Li-Wei Zhou |
| author_facet | Shan Shen Shi-Liang Liu Ji-Hang Jiang Li-Wei Zhou |
| author_sort | Shan Shen |
| collection | DOAJ |
| description | Abstract “Sanghuang” refers to a group of important traditionally-used medicinal mushrooms belonging to the genus Sanghuangporus. In practice, species of Sanghuangporus referred to in medicinal studies and industry are now differentiated mainly by a BLAST search of GenBank with the ITS barcoding region as a query. However, inappropriately labeled ITS sequences of “Sanghuang” in GenBank restrict accurate species identification and, to some extent, the utilization of these species as medicinal resources. We examined all available 271 ITS sequences related to “Sanghuang” in GenBank including 31 newly submitted sequences from this study. Of these sequences, more than half were mislabeled so we have now corrected the corresponding species names. The mislabeled sequences mainly came from strains utilized by non-taxonomists. Based on the analyses of ITS sequences submitted by taxonomists as well as morphological characters, we separate the newly described Sanghuangporus subbaumii from S. baumii and treat S. toxicodendri as a later synonym of S. quercicola. Fourteen species of Sanghuangporus are accepted, with intraspecific distances up to 1.30% (except in S. vaninii, S. weirianus and S. zonatus) and interspecific distances above 1.30% (except between S. alpinus and S. lonicerinus, and S. baumii and S. subbaumii). To stabilize the concept of these 14 species of Sanghuangporus, their taxonomic information and reliable ITS reference sequences are provided. Moreover, ten potential diagnostic sequences are provided for Hyperbranched Rolling Circle Amplification to rapidly confirm three common commercial species, viz. S. baumii, S. sanghuang, and S. vaninii. Our results provide a practical method for ITS barcoding-based species identification of Sanghuangporus and will promote medicinal studies and commercial development from taxonomically correct material. |
| first_indexed | 2024-12-17T22:38:14Z |
| format | Article |
| id | doaj.art-197129c99d0844de8ce7c2ff8c2835bd |
| institution | Directory Open Access Journal |
| issn | 2210-6359 |
| language | English |
| last_indexed | 2024-12-17T22:38:14Z |
| publishDate | 2021-04-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj.art-197129c99d0844de8ce7c2ff8c2835bd2022-12-21T21:30:02ZengBMCIMA Fungus2210-63592021-04-0112112110.1186/s43008-021-00059-xAddressing widespread misidentifications of traditional medicinal mushrooms in Sanghuangporus (Basidiomycota) through ITS barcoding and designation of reference sequencesShan Shen0Shi-Liang Liu1Ji-Hang Jiang2Li-Wei Zhou3State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of SciencesAbstract “Sanghuang” refers to a group of important traditionally-used medicinal mushrooms belonging to the genus Sanghuangporus. In practice, species of Sanghuangporus referred to in medicinal studies and industry are now differentiated mainly by a BLAST search of GenBank with the ITS barcoding region as a query. However, inappropriately labeled ITS sequences of “Sanghuang” in GenBank restrict accurate species identification and, to some extent, the utilization of these species as medicinal resources. We examined all available 271 ITS sequences related to “Sanghuang” in GenBank including 31 newly submitted sequences from this study. Of these sequences, more than half were mislabeled so we have now corrected the corresponding species names. The mislabeled sequences mainly came from strains utilized by non-taxonomists. Based on the analyses of ITS sequences submitted by taxonomists as well as morphological characters, we separate the newly described Sanghuangporus subbaumii from S. baumii and treat S. toxicodendri as a later synonym of S. quercicola. Fourteen species of Sanghuangporus are accepted, with intraspecific distances up to 1.30% (except in S. vaninii, S. weirianus and S. zonatus) and interspecific distances above 1.30% (except between S. alpinus and S. lonicerinus, and S. baumii and S. subbaumii). To stabilize the concept of these 14 species of Sanghuangporus, their taxonomic information and reliable ITS reference sequences are provided. Moreover, ten potential diagnostic sequences are provided for Hyperbranched Rolling Circle Amplification to rapidly confirm three common commercial species, viz. S. baumii, S. sanghuang, and S. vaninii. Our results provide a practical method for ITS barcoding-based species identification of Sanghuangporus and will promote medicinal studies and commercial development from taxonomically correct material.https://doi.org/10.1186/s43008-021-00059-xHymenochaetaceaePhylogenySpecies boundaryTaxonomyWood-inhabiting fungiOne new taxon |
| spellingShingle | Shan Shen Shi-Liang Liu Ji-Hang Jiang Li-Wei Zhou Addressing widespread misidentifications of traditional medicinal mushrooms in Sanghuangporus (Basidiomycota) through ITS barcoding and designation of reference sequences IMA Fungus Hymenochaetaceae Phylogeny Species boundary Taxonomy Wood-inhabiting fungi One new taxon |
| title | Addressing widespread misidentifications of traditional medicinal mushrooms in Sanghuangporus (Basidiomycota) through ITS barcoding and designation of reference sequences |
| title_full | Addressing widespread misidentifications of traditional medicinal mushrooms in Sanghuangporus (Basidiomycota) through ITS barcoding and designation of reference sequences |
| title_fullStr | Addressing widespread misidentifications of traditional medicinal mushrooms in Sanghuangporus (Basidiomycota) through ITS barcoding and designation of reference sequences |
| title_full_unstemmed | Addressing widespread misidentifications of traditional medicinal mushrooms in Sanghuangporus (Basidiomycota) through ITS barcoding and designation of reference sequences |
| title_short | Addressing widespread misidentifications of traditional medicinal mushrooms in Sanghuangporus (Basidiomycota) through ITS barcoding and designation of reference sequences |
| title_sort | addressing widespread misidentifications of traditional medicinal mushrooms in sanghuangporus basidiomycota through its barcoding and designation of reference sequences |
| topic | Hymenochaetaceae Phylogeny Species boundary Taxonomy Wood-inhabiting fungi One new taxon |
| url | https://doi.org/10.1186/s43008-021-00059-x |
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