Next Generation Sequencing in Cytopathology: Focus on Non-Small Cell Lung Cancer
Molecular cytopathology is a rapidly evolving field embracing both conventional microscopy and molecular pathology. Its growing popularity stems from the fact that in many types of advanced cancers, including non small cell lung cancer (NSCLC), cytological samples often constitute the only available...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-02-01
|
| Series: | Frontiers in Medicine |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2021.633923/full |
| _version_ | 1818916603671085056 |
|---|---|
| author | Pasquale Pisapia Francesco Pepe Antonino Iaccarino Roberta Sgariglia Mariantonia Nacchio Floriana Conticelli Maria Salatiello Rossella Tufano Gianluca Russo Gianluca Gragnano Ilaria Girolami Albino Eccher Umberto Malapelle Giancarlo Troncone |
| author_facet | Pasquale Pisapia Francesco Pepe Antonino Iaccarino Roberta Sgariglia Mariantonia Nacchio Floriana Conticelli Maria Salatiello Rossella Tufano Gianluca Russo Gianluca Gragnano Ilaria Girolami Albino Eccher Umberto Malapelle Giancarlo Troncone |
| author_sort | Pasquale Pisapia |
| collection | DOAJ |
| description | Molecular cytopathology is a rapidly evolving field embracing both conventional microscopy and molecular pathology. Its growing popularity stems from the fact that in many types of advanced cancers, including non small cell lung cancer (NSCLC), cytological samples often constitute the only available specimens for morphomolecular analysis. Indeed, non formalin fixed and paraffin embedded (FFPE) cytological samples feature a higher quality of extracted nucleic acids than histological specimens. However, because of the growing complexity of molecular testing, several efforts should be made to validate the analytical performance of the wide array of currently available molecular technologies, including next generation sequencing (NGS). This technology has the terrific advantage of allowing simultaneous detection of scores of predictive biomarkers even in low-input DNA/RNA specimens. Here, we briefly review the role of the modern cytopathologist in the morphomolecular diagnosing of advanced stage NSCLC and the adoption of NGS in conventional cytopreparations (cell blocks, direct smears, and liquid-based cytology) and supernatants. |
| first_indexed | 2024-12-20T00:20:48Z |
| format | Article |
| id | doaj.art-197214dfb4c04874966ee0ec43e3ab09 |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-12-20T00:20:48Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-197214dfb4c04874966ee0ec43e3ab092022-12-21T20:00:12ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2021-02-01810.3389/fmed.2021.633923633923Next Generation Sequencing in Cytopathology: Focus on Non-Small Cell Lung CancerPasquale Pisapia0Francesco Pepe1Antonino Iaccarino2Roberta Sgariglia3Mariantonia Nacchio4Floriana Conticelli5Maria Salatiello6Rossella Tufano7Gianluca Russo8Gianluca Gragnano9Ilaria Girolami10Albino Eccher11Umberto Malapelle12Giancarlo Troncone13Department of Public Health, University of Naples Federico II, Naples, ItalyDepartment of Public Health, University of Naples Federico II, Naples, ItalyDepartment of Public Health, University of Naples Federico II, Naples, ItalyDepartment of Public Health, University of Naples Federico II, Naples, ItalyDepartment of Public Health, University of Naples Federico II, Naples, ItalyDepartment of Public Health, University of Naples Federico II, Naples, ItalyDepartment of Public Health, University of Naples Federico II, Naples, ItalyCEINGE Biotecnologie Avanzate, Naples, ItalyDepartment of Public Health, University of Naples Federico II, Naples, ItalyDepartment of Public Health, University of Naples Federico II, Naples, ItalyDivision of Pathology, Central Hospital Bolzano, Bolzano, ItalyDepartment of Pathology and Diagnostics, University and Hospital Trust of Verona, Verona, ItalyDepartment of Public Health, University of Naples Federico II, Naples, ItalyDepartment of Public Health, University of Naples Federico II, Naples, ItalyMolecular cytopathology is a rapidly evolving field embracing both conventional microscopy and molecular pathology. Its growing popularity stems from the fact that in many types of advanced cancers, including non small cell lung cancer (NSCLC), cytological samples often constitute the only available specimens for morphomolecular analysis. Indeed, non formalin fixed and paraffin embedded (FFPE) cytological samples feature a higher quality of extracted nucleic acids than histological specimens. However, because of the growing complexity of molecular testing, several efforts should be made to validate the analytical performance of the wide array of currently available molecular technologies, including next generation sequencing (NGS). This technology has the terrific advantage of allowing simultaneous detection of scores of predictive biomarkers even in low-input DNA/RNA specimens. Here, we briefly review the role of the modern cytopathologist in the morphomolecular diagnosing of advanced stage NSCLC and the adoption of NGS in conventional cytopreparations (cell blocks, direct smears, and liquid-based cytology) and supernatants.https://www.frontiersin.org/articles/10.3389/fmed.2021.633923/fullmolecular cytopathologycytopathologyNSCLCfine needle aspirationcell blocksmear |
| spellingShingle | Pasquale Pisapia Francesco Pepe Antonino Iaccarino Roberta Sgariglia Mariantonia Nacchio Floriana Conticelli Maria Salatiello Rossella Tufano Gianluca Russo Gianluca Gragnano Ilaria Girolami Albino Eccher Umberto Malapelle Giancarlo Troncone Next Generation Sequencing in Cytopathology: Focus on Non-Small Cell Lung Cancer Frontiers in Medicine molecular cytopathology cytopathology NSCLC fine needle aspiration cell block smear |
| title | Next Generation Sequencing in Cytopathology: Focus on Non-Small Cell Lung Cancer |
| title_full | Next Generation Sequencing in Cytopathology: Focus on Non-Small Cell Lung Cancer |
| title_fullStr | Next Generation Sequencing in Cytopathology: Focus on Non-Small Cell Lung Cancer |
| title_full_unstemmed | Next Generation Sequencing in Cytopathology: Focus on Non-Small Cell Lung Cancer |
| title_short | Next Generation Sequencing in Cytopathology: Focus on Non-Small Cell Lung Cancer |
| title_sort | next generation sequencing in cytopathology focus on non small cell lung cancer |
| topic | molecular cytopathology cytopathology NSCLC fine needle aspiration cell block smear |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2021.633923/full |
| work_keys_str_mv | AT pasqualepisapia nextgenerationsequencingincytopathologyfocusonnonsmallcelllungcancer AT francescopepe nextgenerationsequencingincytopathologyfocusonnonsmallcelllungcancer AT antoninoiaccarino nextgenerationsequencingincytopathologyfocusonnonsmallcelllungcancer AT robertasgariglia nextgenerationsequencingincytopathologyfocusonnonsmallcelllungcancer AT mariantonianacchio nextgenerationsequencingincytopathologyfocusonnonsmallcelllungcancer AT florianaconticelli nextgenerationsequencingincytopathologyfocusonnonsmallcelllungcancer AT mariasalatiello nextgenerationsequencingincytopathologyfocusonnonsmallcelllungcancer AT rossellatufano nextgenerationsequencingincytopathologyfocusonnonsmallcelllungcancer AT gianlucarusso nextgenerationsequencingincytopathologyfocusonnonsmallcelllungcancer AT gianlucagragnano nextgenerationsequencingincytopathologyfocusonnonsmallcelllungcancer AT ilariagirolami nextgenerationsequencingincytopathologyfocusonnonsmallcelllungcancer AT albinoeccher nextgenerationsequencingincytopathologyfocusonnonsmallcelllungcancer AT umbertomalapelle nextgenerationsequencingincytopathologyfocusonnonsmallcelllungcancer AT giancarlotroncone nextgenerationsequencingincytopathologyfocusonnonsmallcelllungcancer |