Role of microtubule dynamics in Wallerian degeneration and nerve regeneration after peripheral nerve injury
Wallerian degeneration, the progressive disintegration of distal axons and myelin that occurs after peripheral nerve injury, is essential for creating a permissive microenvironment for nerve regeneration, and involves cytoskeletal reconstruction. However, it is unclear whether microtubule dynamics p...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=3;spage=673;epage=681;aulast=Liu |
| _version_ | 1831537091867049984 |
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| author | Jingmin Liu Lixia Li Ying Zou Lanya Fu Xinrui Ma Haowen Zhang Yizhou Xu Jiawei Xu Jiaqi Zhang Mi Li Xiaofang Hu Zhenlin Li Xianghai Wang Hao Sun Hui Zheng Lixin Zhu Jiasong Guo |
| author_facet | Jingmin Liu Lixia Li Ying Zou Lanya Fu Xinrui Ma Haowen Zhang Yizhou Xu Jiawei Xu Jiaqi Zhang Mi Li Xiaofang Hu Zhenlin Li Xianghai Wang Hao Sun Hui Zheng Lixin Zhu Jiasong Guo |
| author_sort | Jingmin Liu |
| collection | DOAJ |
| description | Wallerian degeneration, the progressive disintegration of distal axons and myelin that occurs after peripheral nerve injury, is essential for creating a permissive microenvironment for nerve regeneration, and involves cytoskeletal reconstruction. However, it is unclear whether microtubule dynamics play a role in this process. To address this, we treated cultured sciatic nerve explants, an in vitro model of Wallerian degeneration, with the microtubule-targeting agents paclitaxel and nocodazole. We found that paclitaxel-induced microtubule stabilization promoted axon and myelin degeneration and Schwann cell dedifferentiation, whereas nocodazole-induced microtubule destabilization inhibited these processes. Evaluation of an in vivo model of peripheral nerve injury showed that treatment with paclitaxel or nocodazole accelerated or attenuated axonal regeneration, as well as functional recovery of nerve conduction and target muscle and motor behavior, respectively. These results suggest that microtubule dynamics participate in peripheral nerve regeneration after injury by affecting Wallerian degeneration. This study was approved by the Animal Care and Use Committee of Southern Medical University, China (approval No. SMU-L2015081) on October 15, 2015. |
| first_indexed | 2024-12-16T23:00:33Z |
| format | Article |
| id | doaj.art-1972ab37084f4739be74e0ad46a87173 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-16T23:00:33Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-1972ab37084f4739be74e0ad46a871732022-12-21T22:12:45ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742022-01-0117367368110.4103/1673-5374.320997Role of microtubule dynamics in Wallerian degeneration and nerve regeneration after peripheral nerve injuryJingmin LiuLixia LiYing ZouLanya FuXinrui MaHaowen ZhangYizhou XuJiawei XuJiaqi ZhangMi LiXiaofang HuZhenlin LiXianghai WangHao SunHui ZhengLixin ZhuJiasong GuoWallerian degeneration, the progressive disintegration of distal axons and myelin that occurs after peripheral nerve injury, is essential for creating a permissive microenvironment for nerve regeneration, and involves cytoskeletal reconstruction. However, it is unclear whether microtubule dynamics play a role in this process. To address this, we treated cultured sciatic nerve explants, an in vitro model of Wallerian degeneration, with the microtubule-targeting agents paclitaxel and nocodazole. We found that paclitaxel-induced microtubule stabilization promoted axon and myelin degeneration and Schwann cell dedifferentiation, whereas nocodazole-induced microtubule destabilization inhibited these processes. Evaluation of an in vivo model of peripheral nerve injury showed that treatment with paclitaxel or nocodazole accelerated or attenuated axonal regeneration, as well as functional recovery of nerve conduction and target muscle and motor behavior, respectively. These results suggest that microtubule dynamics participate in peripheral nerve regeneration after injury by affecting Wallerian degeneration. This study was approved by the Animal Care and Use Committee of Southern Medical University, China (approval No. SMU-L2015081) on October 15, 2015.http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=3;spage=673;epage=681;aulast=Liuaxon; demyelination; microtubule dynamics; nerve regeneration; nocodazole; paclitaxel; peripheral nerve injury; schwann cell; wallerian degeneration |
| spellingShingle | Jingmin Liu Lixia Li Ying Zou Lanya Fu Xinrui Ma Haowen Zhang Yizhou Xu Jiawei Xu Jiaqi Zhang Mi Li Xiaofang Hu Zhenlin Li Xianghai Wang Hao Sun Hui Zheng Lixin Zhu Jiasong Guo Role of microtubule dynamics in Wallerian degeneration and nerve regeneration after peripheral nerve injury Neural Regeneration Research axon; demyelination; microtubule dynamics; nerve regeneration; nocodazole; paclitaxel; peripheral nerve injury; schwann cell; wallerian degeneration |
| title | Role of microtubule dynamics in Wallerian degeneration and nerve regeneration after peripheral nerve injury |
| title_full | Role of microtubule dynamics in Wallerian degeneration and nerve regeneration after peripheral nerve injury |
| title_fullStr | Role of microtubule dynamics in Wallerian degeneration and nerve regeneration after peripheral nerve injury |
| title_full_unstemmed | Role of microtubule dynamics in Wallerian degeneration and nerve regeneration after peripheral nerve injury |
| title_short | Role of microtubule dynamics in Wallerian degeneration and nerve regeneration after peripheral nerve injury |
| title_sort | role of microtubule dynamics in wallerian degeneration and nerve regeneration after peripheral nerve injury |
| topic | axon; demyelination; microtubule dynamics; nerve regeneration; nocodazole; paclitaxel; peripheral nerve injury; schwann cell; wallerian degeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=3;spage=673;epage=681;aulast=Liu |
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