Analysis and Comparison of Biochemical and Molecular Structural Characteristics and Phylogenetic Trend of Two h-TypeThioredoxin Isoforms from Grape (Vitis vinifera L. cv. Askari)
Thioredoxins (Trxs) are small heat-stable proteins that participate in dithiol-disulfide exchange reactions. In contrast to other organisms, plants contain six different Trx types: <em>f</em>, <em>m</em>, <em>x</em>, <em>y</em>, <em>o</em>...
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Shahid Bahonar University of Kerman
2010-05-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
| Subjects: | |
| Online Access: | https://jab.uk.ac.ir/article_359_f9f8b918cc0e82a00d3efb60bff87a7a.pdf |
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| author | reza Heydari raheem Haddad GHasssemali Garoosi |
| author_facet | reza Heydari raheem Haddad GHasssemali Garoosi |
| author_sort | reza Heydari |
| collection | DOAJ |
| description | Thioredoxins (Trxs) are small heat-stable proteins that participate in dithiol-disulfide exchange reactions. In contrast to other organisms, plants contain six different Trx types: <em>f</em>, <em>m</em>, <em>x</em>, <em>y</em>, <em>o</em> and <em>h</em>. The <em>h</em>-type Trx consists of multiple forms that involved in different processes such as cellular protection against oxidative, biotic and abiotic stresses. Two thioredoxin <em>h-</em>type genes, called <em>VvTrxh4</em> and <em>VvCxxS2</em>, were isolated of grape (<em>Vitis vinifera </em>L. cv. Askari) berry tissue and were cloned into pUC19 plasmid vector. Reverse transcription was carried out using Polymerase Chain Reaction (RT-PCR), to analyze biochemical, structural and phylogenetic characteristics. Nucleotide sequence analysis revealed that the open reading frame of <em>VvTrxh4</em> and <em>VvCxxS2</em> genes are 345 bp and 381 bp long, respectively and encode for proteins of 114 and 126 amino acid residues, respectively. Protein sequence analysis showed that <em>VvTrxh4</em> gene contains a typical catalytic site WCGPC, whereas <em>VvCxxS2</em> gene harbors the non-typical active site WCIPS. The calculated molecular mass and the predicted isoelectric point of the deduced polypeptides for both VvTrxh4 and VvCxxS2 were 12.76 kDa and 5.22, and also 14.25 kDa and 4.68, respectively. Structural analysis showed that deduced proteins contain a identical 3D structure, whereas phylogenetic study of cloned genes with thioredoxin from other plants revealed that these genes are belonging to different subgroups. In the present research project, <em>VvTrxh4</em> gene belongs to class IA, while <em>VvCxxS2</em> gene belongs to class IIIB from <em>h</em>-type thioredoxins |
| first_indexed | 2024-12-10T22:44:57Z |
| format | Article |
| id | doaj.art-19739c10adc04ff9ac83fee1316bdd67 |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-12-10T22:44:57Z |
| publishDate | 2010-05-01 |
| publisher | Shahid Bahonar University of Kerman |
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| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-19739c10adc04ff9ac83fee1316bdd672022-12-22T01:30:36ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002010-05-0121254310.22103/jab.2011.359359Analysis and Comparison of Biochemical and Molecular Structural Characteristics and Phylogenetic Trend of Two h-TypeThioredoxin Isoforms from Grape (Vitis vinifera L. cv. Askari)reza Heydari0raheem Haddad1GHasssemali Garoosi2کارشناس ارشد گروه بیوتکنولوژی کشاورزی دانشکده فنی ومهندسی دانشگاه بین المللی امام خمینی قزوینعضوهیات علمی گروه بیوتکنولوژی کشاورزی دانشکده فنی و مهندسی دانشگاه بین المللی امام خمینی قزوینعضوهیات علمی گروه بیوتکنولوژی کشاورزی دانشکده فنی ومهندسی دانشگاه بین المللی امام خمینی قزوینThioredoxins (Trxs) are small heat-stable proteins that participate in dithiol-disulfide exchange reactions. In contrast to other organisms, plants contain six different Trx types: <em>f</em>, <em>m</em>, <em>x</em>, <em>y</em>, <em>o</em> and <em>h</em>. The <em>h</em>-type Trx consists of multiple forms that involved in different processes such as cellular protection against oxidative, biotic and abiotic stresses. Two thioredoxin <em>h-</em>type genes, called <em>VvTrxh4</em> and <em>VvCxxS2</em>, were isolated of grape (<em>Vitis vinifera </em>L. cv. Askari) berry tissue and were cloned into pUC19 plasmid vector. Reverse transcription was carried out using Polymerase Chain Reaction (RT-PCR), to analyze biochemical, structural and phylogenetic characteristics. Nucleotide sequence analysis revealed that the open reading frame of <em>VvTrxh4</em> and <em>VvCxxS2</em> genes are 345 bp and 381 bp long, respectively and encode for proteins of 114 and 126 amino acid residues, respectively. Protein sequence analysis showed that <em>VvTrxh4</em> gene contains a typical catalytic site WCGPC, whereas <em>VvCxxS2</em> gene harbors the non-typical active site WCIPS. The calculated molecular mass and the predicted isoelectric point of the deduced polypeptides for both VvTrxh4 and VvCxxS2 were 12.76 kDa and 5.22, and also 14.25 kDa and 4.68, respectively. Structural analysis showed that deduced proteins contain a identical 3D structure, whereas phylogenetic study of cloned genes with thioredoxin from other plants revealed that these genes are belonging to different subgroups. In the present research project, <em>VvTrxh4</em> gene belongs to class IA, while <em>VvCxxS2</em> gene belongs to class IIIB from <em>h</em>-type thioredoxinshttps://jab.uk.ac.ir/article_359_f9f8b918cc0e82a00d3efb60bff87a7a.pdfbiochemical characteristicscloninggrapephylogeneticthioredoxin |
| spellingShingle | reza Heydari raheem Haddad GHasssemali Garoosi Analysis and Comparison of Biochemical and Molecular Structural Characteristics and Phylogenetic Trend of Two h-TypeThioredoxin Isoforms from Grape (Vitis vinifera L. cv. Askari) مجله بیوتکنولوژی کشاورزی biochemical characteristics cloning grape phylogenetic thioredoxin |
| title | Analysis and Comparison of Biochemical and Molecular Structural Characteristics and Phylogenetic Trend of Two h-TypeThioredoxin Isoforms from Grape (Vitis vinifera L. cv. Askari) |
| title_full | Analysis and Comparison of Biochemical and Molecular Structural Characteristics and Phylogenetic Trend of Two h-TypeThioredoxin Isoforms from Grape (Vitis vinifera L. cv. Askari) |
| title_fullStr | Analysis and Comparison of Biochemical and Molecular Structural Characteristics and Phylogenetic Trend of Two h-TypeThioredoxin Isoforms from Grape (Vitis vinifera L. cv. Askari) |
| title_full_unstemmed | Analysis and Comparison of Biochemical and Molecular Structural Characteristics and Phylogenetic Trend of Two h-TypeThioredoxin Isoforms from Grape (Vitis vinifera L. cv. Askari) |
| title_short | Analysis and Comparison of Biochemical and Molecular Structural Characteristics and Phylogenetic Trend of Two h-TypeThioredoxin Isoforms from Grape (Vitis vinifera L. cv. Askari) |
| title_sort | analysis and comparison of biochemical and molecular structural characteristics and phylogenetic trend of two h typethioredoxin isoforms from grape vitis vinifera l cv askari |
| topic | biochemical characteristics cloning grape phylogenetic thioredoxin |
| url | https://jab.uk.ac.ir/article_359_f9f8b918cc0e82a00d3efb60bff87a7a.pdf |
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