Effect of Nanopriming with Selenium Nanocomposites on Potato Productivity in a Field Experiment, Soybean Germination and Viability of <i>Pectobacterium carotovorum</i>
In recent decades, the use of nanocomposites (NCs) in crop production has been actively studied. We have previously shown that chemically synthesized selenium nanocomposites (Se NCs) based on natural polymeric matrices of arabinogalactan (Se/AG NCs), carrageenan (Se/CAR NCs), and starch (Se/ST NCs)...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/9/4/458 |
| _version_ | 1797605202371543040 |
|---|---|
| author | Alla I. Perfileva Anastasia R. Kharasova Olga A. Nozhkina Alexander V. Sidorov Irina A. Graskova Konstantin V. Krutovsky |
| author_facet | Alla I. Perfileva Anastasia R. Kharasova Olga A. Nozhkina Alexander V. Sidorov Irina A. Graskova Konstantin V. Krutovsky |
| author_sort | Alla I. Perfileva |
| collection | DOAJ |
| description | In recent decades, the use of nanocomposites (NCs) in crop production has been actively studied. We have previously shown that chemically synthesized selenium nanocomposites (Se NCs) based on natural polymeric matrices of arabinogalactan (Se/AG NCs), carrageenan (Se/CAR NCs), and starch (Se/ST NCs) stimulated the growth and development of potatoes in vitro and had an antibacterial effect against the phytopathogen <i>Clavibacter sepedonicus</i>. It is important to confirm that the results obtained in the laboratory are also reproducible in the field conditions of crop cultivation. In addition, the applications of NCs can be expanded if a wider range of their biological activity is revealed, and the effect on other types of cultivated plants and phytopathogens is shown. In this regard, the purpose of this research was to study the effect of nanopriming with Se NCs on the productivity of potatoes in a field experiment, the germination of soybeans, and the viability of the phytopathogen <i>Pectobacterium carotovorum</i> under in vitro conditions. The study included the use of traditional methods of conducting a field experiment in natural growing conditions, also using microbiological cultures and studying the bacterial profile from the endosphere of potato tubers by the imprint method, germinating <i>Glycine max</i> L. soybean seeds after nanopriming in Petri dishes, followed by the measurement of morphometric and biochemical parameters such as the activity of antioxidant enzymes and content of diene conjugates. Based on the results of field experiments, it was found that the preplant treatment of tubers with Se/AG and Se/CAR NCs stimulated a significant increase in the number of stems in potato plants and tubers per plant, but for Se/AG NC, the increase was observed only in 2020 and 2022, and the stimulating effect of Se/AG and Se/CAR NCs on the weight of tubers was observed only in 2020. In the yield structure, the proportion of marketable seed tubers was increased under the influence of all Se NCs. Endosphere cultures from tubers showed that bacteria were present in only 56% of the tubers in the control, while 78% of the tubers obtained from plants grown from seed tubers treated with Se/AG NC and 100% of the tubers from plants grown from seed tubers treated with Se/ST and Se/CAR NCs contained bacteria. The bacteria were represented by both spore-forming Gram-positive and Gram-negative bacteria of various sizes. The results presented in this article and previously published data showed that the maximum diversity of bacteria was observed after treatment with Se/ST NC. Experiments on potatoes showed that Se/AG NC had the greatest biological effect. In addition, the growth-stimulating effect of these NCs on the biomass of the roots of soybean seedlings was shown. It activated the antioxidant enzymes and reduced the level of diene conjugates (DCs), the amount of which increased significantly after seed biopriming with the phytopathogen <i>Pectobacterium carotovorum</i>. The antibacterial effect of Se/AG NC in relation to the <i>P. carotovorum</i> was observed as a reduction in the growth of the bacterial culture, biofilm formation, and dehydrogenase activity of cells. Thus, it has been shown that Se/AG NC has a stimulating effect on such agriculturally important crops as potatoes and soybeans, as well as an antibacterial effect against Gram-negative and Gram-positive phytopathogenic bacteria. |
| first_indexed | 2024-03-11T04:57:45Z |
| format | Article |
| id | doaj.art-19765f5d807d49b3a165ab53757eb6d3 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-11T04:57:45Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-19765f5d807d49b3a165ab53757eb6d32023-11-17T19:29:01ZengMDPI AGHorticulturae2311-75242023-04-019445810.3390/horticulturae9040458Effect of Nanopriming with Selenium Nanocomposites on Potato Productivity in a Field Experiment, Soybean Germination and Viability of <i>Pectobacterium carotovorum</i>Alla I. Perfileva0Anastasia R. Kharasova1Olga A. Nozhkina2Alexander V. Sidorov3Irina A. Graskova4Konstantin V. Krutovsky5Laboratory of Plant-Microbe Interactions, Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaFaculty of Biology and Soil Studies, Irkutsk State University, 664011 Irkutsk, RussiaLaboratory of Plant-Microbe Interactions, Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaLaboratory of Plant-Microbe Interactions, Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaLaboratory of Plant-Microbe Interactions, Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences, 664033 Irkutsk, RussiaDepartment of Forest Genetics and Forest Tree Breeding, Faculty of Forest Sciences and Forest Ecology, Georg-August University of Göttingen, Büsgenweg 2, 37077 Göttingen, GermanyIn recent decades, the use of nanocomposites (NCs) in crop production has been actively studied. We have previously shown that chemically synthesized selenium nanocomposites (Se NCs) based on natural polymeric matrices of arabinogalactan (Se/AG NCs), carrageenan (Se/CAR NCs), and starch (Se/ST NCs) stimulated the growth and development of potatoes in vitro and had an antibacterial effect against the phytopathogen <i>Clavibacter sepedonicus</i>. It is important to confirm that the results obtained in the laboratory are also reproducible in the field conditions of crop cultivation. In addition, the applications of NCs can be expanded if a wider range of their biological activity is revealed, and the effect on other types of cultivated plants and phytopathogens is shown. In this regard, the purpose of this research was to study the effect of nanopriming with Se NCs on the productivity of potatoes in a field experiment, the germination of soybeans, and the viability of the phytopathogen <i>Pectobacterium carotovorum</i> under in vitro conditions. The study included the use of traditional methods of conducting a field experiment in natural growing conditions, also using microbiological cultures and studying the bacterial profile from the endosphere of potato tubers by the imprint method, germinating <i>Glycine max</i> L. soybean seeds after nanopriming in Petri dishes, followed by the measurement of morphometric and biochemical parameters such as the activity of antioxidant enzymes and content of diene conjugates. Based on the results of field experiments, it was found that the preplant treatment of tubers with Se/AG and Se/CAR NCs stimulated a significant increase in the number of stems in potato plants and tubers per plant, but for Se/AG NC, the increase was observed only in 2020 and 2022, and the stimulating effect of Se/AG and Se/CAR NCs on the weight of tubers was observed only in 2020. In the yield structure, the proportion of marketable seed tubers was increased under the influence of all Se NCs. Endosphere cultures from tubers showed that bacteria were present in only 56% of the tubers in the control, while 78% of the tubers obtained from plants grown from seed tubers treated with Se/AG NC and 100% of the tubers from plants grown from seed tubers treated with Se/ST and Se/CAR NCs contained bacteria. The bacteria were represented by both spore-forming Gram-positive and Gram-negative bacteria of various sizes. The results presented in this article and previously published data showed that the maximum diversity of bacteria was observed after treatment with Se/ST NC. Experiments on potatoes showed that Se/AG NC had the greatest biological effect. In addition, the growth-stimulating effect of these NCs on the biomass of the roots of soybean seedlings was shown. It activated the antioxidant enzymes and reduced the level of diene conjugates (DCs), the amount of which increased significantly after seed biopriming with the phytopathogen <i>Pectobacterium carotovorum</i>. The antibacterial effect of Se/AG NC in relation to the <i>P. carotovorum</i> was observed as a reduction in the growth of the bacterial culture, biofilm formation, and dehydrogenase activity of cells. Thus, it has been shown that Se/AG NC has a stimulating effect on such agriculturally important crops as potatoes and soybeans, as well as an antibacterial effect against Gram-negative and Gram-positive phytopathogenic bacteria.https://www.mdpi.com/2311-7524/9/4/458potatotubersnanocompositeseleniummicrobial profilebacteria |
| spellingShingle | Alla I. Perfileva Anastasia R. Kharasova Olga A. Nozhkina Alexander V. Sidorov Irina A. Graskova Konstantin V. Krutovsky Effect of Nanopriming with Selenium Nanocomposites on Potato Productivity in a Field Experiment, Soybean Germination and Viability of <i>Pectobacterium carotovorum</i> Horticulturae potato tubers nanocomposite selenium microbial profile bacteria |
| title | Effect of Nanopriming with Selenium Nanocomposites on Potato Productivity in a Field Experiment, Soybean Germination and Viability of <i>Pectobacterium carotovorum</i> |
| title_full | Effect of Nanopriming with Selenium Nanocomposites on Potato Productivity in a Field Experiment, Soybean Germination and Viability of <i>Pectobacterium carotovorum</i> |
| title_fullStr | Effect of Nanopriming with Selenium Nanocomposites on Potato Productivity in a Field Experiment, Soybean Germination and Viability of <i>Pectobacterium carotovorum</i> |
| title_full_unstemmed | Effect of Nanopriming with Selenium Nanocomposites on Potato Productivity in a Field Experiment, Soybean Germination and Viability of <i>Pectobacterium carotovorum</i> |
| title_short | Effect of Nanopriming with Selenium Nanocomposites on Potato Productivity in a Field Experiment, Soybean Germination and Viability of <i>Pectobacterium carotovorum</i> |
| title_sort | effect of nanopriming with selenium nanocomposites on potato productivity in a field experiment soybean germination and viability of i pectobacterium carotovorum i |
| topic | potato tubers nanocomposite selenium microbial profile bacteria |
| url | https://www.mdpi.com/2311-7524/9/4/458 |
| work_keys_str_mv | AT allaiperfileva effectofnanoprimingwithseleniumnanocompositesonpotatoproductivityinafieldexperimentsoybeangerminationandviabilityofipectobacteriumcarotovorumi AT anastasiarkharasova effectofnanoprimingwithseleniumnanocompositesonpotatoproductivityinafieldexperimentsoybeangerminationandviabilityofipectobacteriumcarotovorumi AT olgaanozhkina effectofnanoprimingwithseleniumnanocompositesonpotatoproductivityinafieldexperimentsoybeangerminationandviabilityofipectobacteriumcarotovorumi AT alexandervsidorov effectofnanoprimingwithseleniumnanocompositesonpotatoproductivityinafieldexperimentsoybeangerminationandviabilityofipectobacteriumcarotovorumi AT irinaagraskova effectofnanoprimingwithseleniumnanocompositesonpotatoproductivityinafieldexperimentsoybeangerminationandviabilityofipectobacteriumcarotovorumi AT konstantinvkrutovsky effectofnanoprimingwithseleniumnanocompositesonpotatoproductivityinafieldexperimentsoybeangerminationandviabilityofipectobacteriumcarotovorumi |