Detection of Selected Canine Viruses in Nigerian Free-Ranging Dogs Traded for Meat Consumption
Animal trade favors the spreading of emerging and re-emerging pathogens. Concerns have been previously expressed regarding the risks of dog trade in spreading zoonotic pathogens in Nigeria. However, the role of these dogs in disseminating highly pathogenic canine viruses has not yet been explored. T...
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MDPI AG
2023-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/6/1119 |
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| author | Linda A. Ndiana Gianvito Lanave Costantina Desario Amienwanlen E. Odigie Kelechi G. Madubuike Maria Stella Lucente Chukwuemeka A. Ezeifeka Giovanni Patruno Eleonora Lorusso Gabriella Elia Canio Buonavoglia Nicola Decaro |
| author_facet | Linda A. Ndiana Gianvito Lanave Costantina Desario Amienwanlen E. Odigie Kelechi G. Madubuike Maria Stella Lucente Chukwuemeka A. Ezeifeka Giovanni Patruno Eleonora Lorusso Gabriella Elia Canio Buonavoglia Nicola Decaro |
| author_sort | Linda A. Ndiana |
| collection | DOAJ |
| description | Animal trade favors the spreading of emerging and re-emerging pathogens. Concerns have been previously expressed regarding the risks of dog trade in spreading zoonotic pathogens in Nigeria. However, the role of these dogs in disseminating highly pathogenic canine viruses has not yet been explored. The present study aimed to identify selected canine viruses in dogs traded for meat consumption in Nigeria. A total of 100 blood samples were screened for carnivore protoparvovirus-1 (CPPV-1), canine adenovirus 1/2 (CAdV-1/2), canine circovirus (CaCV), and canine distemper virus (CDV) by using real-time PCR and conventional PCR and/or sequencing. CPPV-1 DNA was identified in 83% of canine samples while CaCV DNA and CDV RNA were detected in 14% and 17% of the dog samples, respectively. None of the dogs tested positive for CAdV-1/2. The CaCVs identified in this study clustered along with other European, Asian, and American strains. Moreover, CDV strains identified in Nigeria clustered in a separate lineage with the closest genetic relatedness to the Europe–South America-1 clade. Further surveys prior to and after arrival of dogs at the slaughtering points are required to clarify the real virus burden in these animals. |
| first_indexed | 2024-03-11T07:01:11Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T07:01:11Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-197885f4b1034ca9acafa11ec16041e52023-11-17T09:12:39ZengMDPI AGAnimals2076-26152023-03-01136111910.3390/ani13061119Detection of Selected Canine Viruses in Nigerian Free-Ranging Dogs Traded for Meat ConsumptionLinda A. Ndiana0Gianvito Lanave1Costantina Desario2Amienwanlen E. Odigie3Kelechi G. Madubuike4Maria Stella Lucente5Chukwuemeka A. Ezeifeka6Giovanni Patruno7Eleonora Lorusso8Gabriella Elia9Canio Buonavoglia10Nicola Decaro11Department of Veterinary Medicine, University of Bari, Strada Provinciale per Casamassima Km 3, Valenzano, 70010 Bari, ItalyDepartment of Veterinary Medicine, University of Bari, Strada Provinciale per Casamassima Km 3, Valenzano, 70010 Bari, ItalyDepartment of Veterinary Medicine, University of Bari, Strada Provinciale per Casamassima Km 3, Valenzano, 70010 Bari, ItalyDepartment of Veterinary Medicine, University of Bari, Strada Provinciale per Casamassima Km 3, Valenzano, 70010 Bari, ItalyDepartment of Veterinary Microbiology, College of Veterinary Medicine, Michael Okpara University of Agriculture, Umuhaia Ikot Ekpene Road, Umudike 440101, NigeriaDepartment of Veterinary Medicine, University of Bari, Strada Provinciale per Casamassima Km 3, Valenzano, 70010 Bari, ItalyDepartment of Veterinary Microbiology, College of Veterinary Medicine, Michael Okpara University of Agriculture, Umuhaia Ikot Ekpene Road, Umudike 440101, NigeriaDepartment of Veterinary Medicine, University of Bari, Strada Provinciale per Casamassima Km 3, Valenzano, 70010 Bari, ItalyDepartment of Veterinary Medicine, University of Bari, Strada Provinciale per Casamassima Km 3, Valenzano, 70010 Bari, ItalyDepartment of Veterinary Medicine, University of Bari, Strada Provinciale per Casamassima Km 3, Valenzano, 70010 Bari, ItalyDepartment of Veterinary Medicine, University of Bari, Strada Provinciale per Casamassima Km 3, Valenzano, 70010 Bari, ItalyDepartment of Veterinary Medicine, University of Bari, Strada Provinciale per Casamassima Km 3, Valenzano, 70010 Bari, ItalyAnimal trade favors the spreading of emerging and re-emerging pathogens. Concerns have been previously expressed regarding the risks of dog trade in spreading zoonotic pathogens in Nigeria. However, the role of these dogs in disseminating highly pathogenic canine viruses has not yet been explored. The present study aimed to identify selected canine viruses in dogs traded for meat consumption in Nigeria. A total of 100 blood samples were screened for carnivore protoparvovirus-1 (CPPV-1), canine adenovirus 1/2 (CAdV-1/2), canine circovirus (CaCV), and canine distemper virus (CDV) by using real-time PCR and conventional PCR and/or sequencing. CPPV-1 DNA was identified in 83% of canine samples while CaCV DNA and CDV RNA were detected in 14% and 17% of the dog samples, respectively. None of the dogs tested positive for CAdV-1/2. The CaCVs identified in this study clustered along with other European, Asian, and American strains. Moreover, CDV strains identified in Nigeria clustered in a separate lineage with the closest genetic relatedness to the Europe–South America-1 clade. Further surveys prior to and after arrival of dogs at the slaughtering points are required to clarify the real virus burden in these animals.https://www.mdpi.com/2076-2615/13/6/1119Nigeriadog tradeselected virusesmolecular characterizationgenetic mutations |
| spellingShingle | Linda A. Ndiana Gianvito Lanave Costantina Desario Amienwanlen E. Odigie Kelechi G. Madubuike Maria Stella Lucente Chukwuemeka A. Ezeifeka Giovanni Patruno Eleonora Lorusso Gabriella Elia Canio Buonavoglia Nicola Decaro Detection of Selected Canine Viruses in Nigerian Free-Ranging Dogs Traded for Meat Consumption Animals Nigeria dog trade selected viruses molecular characterization genetic mutations |
| title | Detection of Selected Canine Viruses in Nigerian Free-Ranging Dogs Traded for Meat Consumption |
| title_full | Detection of Selected Canine Viruses in Nigerian Free-Ranging Dogs Traded for Meat Consumption |
| title_fullStr | Detection of Selected Canine Viruses in Nigerian Free-Ranging Dogs Traded for Meat Consumption |
| title_full_unstemmed | Detection of Selected Canine Viruses in Nigerian Free-Ranging Dogs Traded for Meat Consumption |
| title_short | Detection of Selected Canine Viruses in Nigerian Free-Ranging Dogs Traded for Meat Consumption |
| title_sort | detection of selected canine viruses in nigerian free ranging dogs traded for meat consumption |
| topic | Nigeria dog trade selected viruses molecular characterization genetic mutations |
| url | https://www.mdpi.com/2076-2615/13/6/1119 |
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