Unraveling long non-coding RNAs through analysis of high-throughput RNA-sequencing data
Extensive genome-wide transcriptome study mediated by high throughput sequencing technique has revolutionized the study of genetics and epigenetic at unprecedented resolution. The research has revealed that besides protein-coding RNAs, large proportions of mammalian transcriptome includes a heap of...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2017-06-01
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| Series: | Non-coding RNA Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054017300203 |
| _version_ | 1797203277727662080 |
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| author | Rashmi Tripathi Pavan Chakraborty Pritish Kumar Varadwaj |
| author_facet | Rashmi Tripathi Pavan Chakraborty Pritish Kumar Varadwaj |
| author_sort | Rashmi Tripathi |
| collection | DOAJ |
| description | Extensive genome-wide transcriptome study mediated by high throughput sequencing technique has revolutionized the study of genetics and epigenetic at unprecedented resolution. The research has revealed that besides protein-coding RNAs, large proportions of mammalian transcriptome includes a heap of regulatory non protein-coding RNAs, the number encoded within human genome is enigmatic. Many taboos developed in the past categorized these non-coding RNAs as ââdark matterâ and âjunksâ. Breaking the myth, RNA-seq-- a recently developed experimental technique is widely being used for studying non-coding RNAs which has acquired the limelight due to their physiological and pathological significance. The longest member of the ncRNA family-- long non-coding RNAs, acts as stable and functional part of a genome, guiding towards the important clues about the varied biological events like cellular-, structural- processes governing the complexity of an organism. Here, we review the most recent and influential computational approach developed to identify and quantify the long non-coding RNAs serving as an assistant for the users to choose appropriate tools for their specific research. Keywords: Transcriptome, High throughput sequencing, Genetic and epigenetic, Long non-coding RNA, RNA-sequencing, RNA-seq |
| first_indexed | 2024-04-24T08:16:47Z |
| format | Article |
| id | doaj.art-1978ab4014fa4226b3874c7bdacb39d5 |
| institution | Directory Open Access Journal |
| issn | 2468-0540 |
| language | English |
| last_indexed | 2024-04-24T08:16:47Z |
| publishDate | 2017-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Non-coding RNA Research |
| spelling | doaj.art-1978ab4014fa4226b3874c7bdacb39d52024-04-17T03:21:48ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402017-06-0122111118Unraveling long non-coding RNAs through analysis of high-throughput RNA-sequencing dataRashmi Tripathi0Pavan Chakraborty1Pritish Kumar Varadwaj2Department of Bioinformatics, Indian Institute of Information Technology Allahabad, Allahabad, 211015, UP, IndiaDepartment of Information Technology, Indian Institute of Information Technology Allahabad, Allahabad, 211015, UP, IndiaDepartment of Bioinformatics, Indian Institute of Information Technology Allahabad, Allahabad, 211015, UP, India; Corresponding author.Extensive genome-wide transcriptome study mediated by high throughput sequencing technique has revolutionized the study of genetics and epigenetic at unprecedented resolution. The research has revealed that besides protein-coding RNAs, large proportions of mammalian transcriptome includes a heap of regulatory non protein-coding RNAs, the number encoded within human genome is enigmatic. Many taboos developed in the past categorized these non-coding RNAs as ââdark matterâ and âjunksâ. Breaking the myth, RNA-seq-- a recently developed experimental technique is widely being used for studying non-coding RNAs which has acquired the limelight due to their physiological and pathological significance. The longest member of the ncRNA family-- long non-coding RNAs, acts as stable and functional part of a genome, guiding towards the important clues about the varied biological events like cellular-, structural- processes governing the complexity of an organism. Here, we review the most recent and influential computational approach developed to identify and quantify the long non-coding RNAs serving as an assistant for the users to choose appropriate tools for their specific research. Keywords: Transcriptome, High throughput sequencing, Genetic and epigenetic, Long non-coding RNA, RNA-sequencing, RNA-seqhttp://www.sciencedirect.com/science/article/pii/S2468054017300203 |
| spellingShingle | Rashmi Tripathi Pavan Chakraborty Pritish Kumar Varadwaj Unraveling long non-coding RNAs through analysis of high-throughput RNA-sequencing data Non-coding RNA Research |
| title | Unraveling long non-coding RNAs through analysis of high-throughput RNA-sequencing data |
| title_full | Unraveling long non-coding RNAs through analysis of high-throughput RNA-sequencing data |
| title_fullStr | Unraveling long non-coding RNAs through analysis of high-throughput RNA-sequencing data |
| title_full_unstemmed | Unraveling long non-coding RNAs through analysis of high-throughput RNA-sequencing data |
| title_short | Unraveling long non-coding RNAs through analysis of high-throughput RNA-sequencing data |
| title_sort | unraveling long non coding rnas through analysis of high throughput rna sequencing data |
| url | http://www.sciencedirect.com/science/article/pii/S2468054017300203 |
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