Expression of Septin4 in Schistosoma japonicum-infected mouse livers after praziquantel treatment
Abstract Background Septin4 (SEPT4) exists widely in human tissues and is related to mechanical stability, actin dynamics, membrane trafficking, viral replication and apoptosis. Data from many studies have suggested that SEPT4 plays a significant role in liver fibrosis. SEPT4 is down-regulated in th...
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| Format: | Article |
| Language: | English |
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BMC
2015-01-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-015-0640-9 |
| _version_ | 1797811798739517440 |
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| author | Dandan Zhu Ke Song Jinling Chen Jianxin Wang Xiaolei Sun Hongyan Qian Xijuan Gu Lingbo Zhang Yongwei Qin Yinong Duan |
| author_facet | Dandan Zhu Ke Song Jinling Chen Jianxin Wang Xiaolei Sun Hongyan Qian Xijuan Gu Lingbo Zhang Yongwei Qin Yinong Duan |
| author_sort | Dandan Zhu |
| collection | DOAJ |
| description | Abstract Background Septin4 (SEPT4) exists widely in human tissues and is related to mechanical stability, actin dynamics, membrane trafficking, viral replication and apoptosis. Data from many studies have suggested that SEPT4 plays a significant role in liver fibrosis. SEPT4 is down-regulated in the model of CCl4 and BDL treated liver fibrosis. However, it is up-regulated and peaked at 12 weeks post-infection (p.i.), and then decreased subsequently in Schistosoma japonicum (S. japonicum) egg-induced liver fibrosis. The aim of this study was to observe the dynamic alteration of SEPT4 after the treatment of praziquantel (PZQ) in ICR mice infected with S. japonicum. Methods Expression of SEPT4 was determined by western blot, immunofluorescence and qRT-PCR. And pro-inflammatory cytokines IL-6 and TNF-α were detected by qRT-PCR. The number of eggs, the diameter of egg granulomas and fibrosis-associated genes were also measured. Results Our results showed that the granulomatous inflammation was reduced, whereafter the expression of SEPT4 on hepatic stellate cells (HSCs) was decreased after PZQ anti-schistosome therapy. And the variation tendency of SEPT4 had positive correlation with the inflammatory response in the area of S. japonicum egg granulomas. Conclusions Based on these findings, the inhibition of the expression of the SEPT4 by PZQ might be due to alleviation of the inflammatory response at the chronic and advanced stage of S. japonicum infection. |
| first_indexed | 2024-03-13T07:28:06Z |
| format | Article |
| id | doaj.art-19794bc50b044480829abc3f8b0be84c |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:28:06Z |
| publishDate | 2015-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-19794bc50b044480829abc3f8b0be84c2023-06-04T11:13:36ZengBMCParasites & Vectors1756-33052015-01-01811710.1186/s13071-015-0640-9Expression of Septin4 in Schistosoma japonicum-infected mouse livers after praziquantel treatmentDandan Zhu0Ke Song1Jinling Chen2Jianxin Wang3Xiaolei Sun4Hongyan Qian5Xijuan Gu6Lingbo Zhang7Yongwei Qin8Yinong Duan9Department of Pathogen Biology, School of Medicine, Nantong UniversityDepartment of Pathogen Biology, School of Medicine, Nantong UniversityDepartment of Pathogen Biology, School of Medicine, Nantong UniversityDepartment of Pathogen Biology, School of Medicine, Nantong UniversityDepartment of Pathogen Biology, School of Medicine, Nantong UniversityCancer Research Center, Affiliated Tumor Hospital of Nantong UniversityNantong University Xinglin CollegeDepartment of Pathogen Biology, School of Medicine, Nantong UniversityDepartment of Pathogen Biology, School of Medicine, Nantong UniversityDepartment of Pathogen Biology, School of Medicine, Nantong UniversityAbstract Background Septin4 (SEPT4) exists widely in human tissues and is related to mechanical stability, actin dynamics, membrane trafficking, viral replication and apoptosis. Data from many studies have suggested that SEPT4 plays a significant role in liver fibrosis. SEPT4 is down-regulated in the model of CCl4 and BDL treated liver fibrosis. However, it is up-regulated and peaked at 12 weeks post-infection (p.i.), and then decreased subsequently in Schistosoma japonicum (S. japonicum) egg-induced liver fibrosis. The aim of this study was to observe the dynamic alteration of SEPT4 after the treatment of praziquantel (PZQ) in ICR mice infected with S. japonicum. Methods Expression of SEPT4 was determined by western blot, immunofluorescence and qRT-PCR. And pro-inflammatory cytokines IL-6 and TNF-α were detected by qRT-PCR. The number of eggs, the diameter of egg granulomas and fibrosis-associated genes were also measured. Results Our results showed that the granulomatous inflammation was reduced, whereafter the expression of SEPT4 on hepatic stellate cells (HSCs) was decreased after PZQ anti-schistosome therapy. And the variation tendency of SEPT4 had positive correlation with the inflammatory response in the area of S. japonicum egg granulomas. Conclusions Based on these findings, the inhibition of the expression of the SEPT4 by PZQ might be due to alleviation of the inflammatory response at the chronic and advanced stage of S. japonicum infection.https://doi.org/10.1186/s13071-015-0640-9Schistosoma japonicumSEPT4InflammationLiver fibrosis |
| spellingShingle | Dandan Zhu Ke Song Jinling Chen Jianxin Wang Xiaolei Sun Hongyan Qian Xijuan Gu Lingbo Zhang Yongwei Qin Yinong Duan Expression of Septin4 in Schistosoma japonicum-infected mouse livers after praziquantel treatment Parasites & Vectors Schistosoma japonicum SEPT4 Inflammation Liver fibrosis |
| title | Expression of Septin4 in Schistosoma japonicum-infected mouse livers after praziquantel treatment |
| title_full | Expression of Septin4 in Schistosoma japonicum-infected mouse livers after praziquantel treatment |
| title_fullStr | Expression of Septin4 in Schistosoma japonicum-infected mouse livers after praziquantel treatment |
| title_full_unstemmed | Expression of Septin4 in Schistosoma japonicum-infected mouse livers after praziquantel treatment |
| title_short | Expression of Septin4 in Schistosoma japonicum-infected mouse livers after praziquantel treatment |
| title_sort | expression of septin4 in schistosoma japonicum infected mouse livers after praziquantel treatment |
| topic | Schistosoma japonicum SEPT4 Inflammation Liver fibrosis |
| url | https://doi.org/10.1186/s13071-015-0640-9 |
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