Fostering Growth in <i>Cinnamomum kanehirae</i> Cuttings: The Beneficial Role of Dark Septate Endophytes in Forest Nursery Management
Root development is critical to successful establishment after seedlings are out-planted on a forest restoration site. However, the restoration of an endangered <i>Cinnamomum kanehirae</i> using cuttings was limited by the lack of axial roots. Dark septate endophytes (DSEs) are an import...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/15/1/16 |
| _version_ | 1797343996902637568 |
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| author | Lei-Chen Lin Hao-Yu Chen Wan-Rou Lin |
| author_facet | Lei-Chen Lin Hao-Yu Chen Wan-Rou Lin |
| author_sort | Lei-Chen Lin |
| collection | DOAJ |
| description | Root development is critical to successful establishment after seedlings are out-planted on a forest restoration site. However, the restoration of an endangered <i>Cinnamomum kanehirae</i> using cuttings was limited by the lack of axial roots. Dark septate endophytes (DSEs) are an important group of asexual filamentous ascomycetous fungi and could promote the performance of host plants. In the current study, we explored the effects of four DSE strains (<i>Melnikomyces</i> sp., <i>Acrocalymma vagum</i>, <i>Wiesneriomyces</i> sp., and <i>Tricholomataceae</i> sp.) on the growth of <i>C. kanehirae</i> cuttings under nursery conditions. The results show that four DSE isolates are able to form symbiotic relationships with <i>C. kanehira</i>, enhancing the seedling height, fresh weight, and chlorophyll concentrations. Notably, the <i>Melnikomyces</i> sp. (DB5) showed significant improvements, secreting peroxidase and indole acetic acid. To facilitate the detection of DB5 within the host roots, we developed specific primers (DB5-1F/DB5-1R). We recommend the adoption of the endophyte inoculation approach and molecular detection methods in forestry nurseries as valuable tools to enhance silvicultural practices and contribute to the conservation of <i>C. kanehirae</i>. |
| first_indexed | 2024-03-08T10:55:54Z |
| format | Article |
| id | doaj.art-1979ec2dcf0f4648bedd8f02553a7a70 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-08T10:55:54Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-1979ec2dcf0f4648bedd8f02553a7a702024-01-26T16:30:37ZengMDPI AGForests1999-49072023-12-011511610.3390/f15010016Fostering Growth in <i>Cinnamomum kanehirae</i> Cuttings: The Beneficial Role of Dark Septate Endophytes in Forest Nursery ManagementLei-Chen Lin0Hao-Yu Chen1Wan-Rou Lin2Department of Forestry and Natural Resources, National Chiayi University, Chiayi 60004, TaiwanDepartment of Forestry and Natural Resources, National Chiayi University, Chiayi 60004, TaiwanBioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 30062, TaiwanRoot development is critical to successful establishment after seedlings are out-planted on a forest restoration site. However, the restoration of an endangered <i>Cinnamomum kanehirae</i> using cuttings was limited by the lack of axial roots. Dark septate endophytes (DSEs) are an important group of asexual filamentous ascomycetous fungi and could promote the performance of host plants. In the current study, we explored the effects of four DSE strains (<i>Melnikomyces</i> sp., <i>Acrocalymma vagum</i>, <i>Wiesneriomyces</i> sp., and <i>Tricholomataceae</i> sp.) on the growth of <i>C. kanehirae</i> cuttings under nursery conditions. The results show that four DSE isolates are able to form symbiotic relationships with <i>C. kanehira</i>, enhancing the seedling height, fresh weight, and chlorophyll concentrations. Notably, the <i>Melnikomyces</i> sp. (DB5) showed significant improvements, secreting peroxidase and indole acetic acid. To facilitate the detection of DB5 within the host roots, we developed specific primers (DB5-1F/DB5-1R). We recommend the adoption of the endophyte inoculation approach and molecular detection methods in forestry nurseries as valuable tools to enhance silvicultural practices and contribute to the conservation of <i>C. kanehirae</i>.https://www.mdpi.com/1999-4907/15/1/16dark septate endophyteDSE responsivenessfungi-plant interactiongrowth promotionnursery |
| spellingShingle | Lei-Chen Lin Hao-Yu Chen Wan-Rou Lin Fostering Growth in <i>Cinnamomum kanehirae</i> Cuttings: The Beneficial Role of Dark Septate Endophytes in Forest Nursery Management Forests dark septate endophyte DSE responsiveness fungi-plant interaction growth promotion nursery |
| title | Fostering Growth in <i>Cinnamomum kanehirae</i> Cuttings: The Beneficial Role of Dark Septate Endophytes in Forest Nursery Management |
| title_full | Fostering Growth in <i>Cinnamomum kanehirae</i> Cuttings: The Beneficial Role of Dark Septate Endophytes in Forest Nursery Management |
| title_fullStr | Fostering Growth in <i>Cinnamomum kanehirae</i> Cuttings: The Beneficial Role of Dark Septate Endophytes in Forest Nursery Management |
| title_full_unstemmed | Fostering Growth in <i>Cinnamomum kanehirae</i> Cuttings: The Beneficial Role of Dark Septate Endophytes in Forest Nursery Management |
| title_short | Fostering Growth in <i>Cinnamomum kanehirae</i> Cuttings: The Beneficial Role of Dark Septate Endophytes in Forest Nursery Management |
| title_sort | fostering growth in i cinnamomum kanehirae i cuttings the beneficial role of dark septate endophytes in forest nursery management |
| topic | dark septate endophyte DSE responsiveness fungi-plant interaction growth promotion nursery |
| url | https://www.mdpi.com/1999-4907/15/1/16 |
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