Mucosal and cellular immune responses elicited by nasal and intramuscular inoculation with ASFV candidate immunogens
African swine fever (ASF) is an infectious disease caused by African swine fever virus (ASFV) that is highly contagious and has an extremely high mortality rate (infected by virulent strains) among domestic and wild pigs, causing huge economic losses to the pig industry globally. In this study, SDS−...
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1200297/full |
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| author | Lulu Xu Lulu Xu Lulu Xu Fei Hao Fei Hao Fei Hao Dae Gwin Jeong Rong Chen Rong Chen Yuan Gan Yuan Gan Lei Zhang Lei Zhang Minjoo Yeom Jong-Woo Lim Yanfei Yu Yanfei Yu Yun Bai Yun Bai Zhiyong Zeng Yongjie Liu Qiyan Xiong Qiyan Xiong Guoqing Shao Guoqing Shao Yuzi Wu Yuzi Wu Zhixin Feng Zhixin Feng Zhixin Feng Zhixin Feng Daesub Song Xing Xie Xing Xie Xing Xie Xing Xie |
| author_facet | Lulu Xu Lulu Xu Lulu Xu Fei Hao Fei Hao Fei Hao Dae Gwin Jeong Rong Chen Rong Chen Yuan Gan Yuan Gan Lei Zhang Lei Zhang Minjoo Yeom Jong-Woo Lim Yanfei Yu Yanfei Yu Yun Bai Yun Bai Zhiyong Zeng Yongjie Liu Qiyan Xiong Qiyan Xiong Guoqing Shao Guoqing Shao Yuzi Wu Yuzi Wu Zhixin Feng Zhixin Feng Zhixin Feng Zhixin Feng Daesub Song Xing Xie Xing Xie Xing Xie Xing Xie |
| author_sort | Lulu Xu |
| collection | DOAJ |
| description | African swine fever (ASF) is an infectious disease caused by African swine fever virus (ASFV) that is highly contagious and has an extremely high mortality rate (infected by virulent strains) among domestic and wild pigs, causing huge economic losses to the pig industry globally. In this study, SDS−PAGE gel bands hybridized with ASFV whole virus protein combined with ASFV-convalescent and ASFV-positive pig serum were identified by mass spectrometry. Six antigens were detected by positive serum reaction bands, and eight antigens were detected in ASFV-convalescent serum. In combination with previous literature reports and proteins corresponding to MHC-II presenting peptides screened from ASFV-positive pig urine conducted in our lab, seven candidate antigens, including KP177R (p22), K78R (p10), CP204L (p30), E183L (p54), B602L (B602L), EP402R-N (CD2V-N) and F317L (F317L), were selected. Subunit-Group 1 was prepared by mixing above-mentioned seven ASFV recombinant proteins with MONTANIDETM1313 VG N mucosal adjuvant and immunizing pigs intranasally and intramuscularly. Subunit-Group 2 was prepared by mixing four ASFV recombinant proteins (p22, p54, CD2V-N1, B602L) with Montanide ISA 51 VG adjuvant and immunizing pigs by intramuscular injection. Anticoagulated whole blood, serum, and oral fluid were collected during immunization for flow cytometry, serum IgG as well as secretory sIgA antibody secretion, and cytokine expression testing to conduct a comprehensive immunogenicity assessment. Both immunogen groups can effectively stimulate the host to produce ideal humoral, mucosal, and cellular immune responses, providing a theoretical basis for subsequent functional studies, such as immunogens challenge protection and elucidation of the pathogenic mechanism of ASFV. |
| first_indexed | 2024-03-12T11:26:54Z |
| format | Article |
| id | doaj.art-197c0a2d04f94928aae5db2604eb9162 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-12T11:26:54Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-197c0a2d04f94928aae5db2604eb91622023-09-01T07:06:12ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-09-011410.3389/fimmu.2023.12002971200297Mucosal and cellular immune responses elicited by nasal and intramuscular inoculation with ASFV candidate immunogensLulu Xu0Lulu Xu1Lulu Xu2Fei Hao3Fei Hao4Fei Hao5Dae Gwin Jeong6Rong Chen7Rong Chen8Yuan Gan9Yuan Gan10Lei Zhang11Lei Zhang12Minjoo Yeom13Jong-Woo Lim14Yanfei Yu15Yanfei Yu16Yun Bai17Yun Bai18Zhiyong Zeng19Yongjie Liu20Qiyan Xiong21Qiyan Xiong22Guoqing Shao23Guoqing Shao24Yuzi Wu25Yuzi Wu26Zhixin Feng27Zhixin Feng28Zhixin Feng29Zhixin Feng30Daesub Song31Xing Xie32Xing Xie33Xing Xie34Xing Xie35Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaKey Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing, ChinaBionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of KoreaKey Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaKey Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaKey Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaCollege of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of KoreaCollege of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of KoreaKey Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaKey Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaCollege of Animal Science, Guizhou University, Guiyang, ChinaJoint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaKey Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaKey Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaJoint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing, ChinaCollege of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of KoreaJoint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory for Veterinary Bio-Product Engineering, Ministry of Agriculture and Rural Affairs, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, ChinaGuoTai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, ChinaJiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing, ChinaAfrican swine fever (ASF) is an infectious disease caused by African swine fever virus (ASFV) that is highly contagious and has an extremely high mortality rate (infected by virulent strains) among domestic and wild pigs, causing huge economic losses to the pig industry globally. In this study, SDS−PAGE gel bands hybridized with ASFV whole virus protein combined with ASFV-convalescent and ASFV-positive pig serum were identified by mass spectrometry. Six antigens were detected by positive serum reaction bands, and eight antigens were detected in ASFV-convalescent serum. In combination with previous literature reports and proteins corresponding to MHC-II presenting peptides screened from ASFV-positive pig urine conducted in our lab, seven candidate antigens, including KP177R (p22), K78R (p10), CP204L (p30), E183L (p54), B602L (B602L), EP402R-N (CD2V-N) and F317L (F317L), were selected. Subunit-Group 1 was prepared by mixing above-mentioned seven ASFV recombinant proteins with MONTANIDETM1313 VG N mucosal adjuvant and immunizing pigs intranasally and intramuscularly. Subunit-Group 2 was prepared by mixing four ASFV recombinant proteins (p22, p54, CD2V-N1, B602L) with Montanide ISA 51 VG adjuvant and immunizing pigs by intramuscular injection. Anticoagulated whole blood, serum, and oral fluid were collected during immunization for flow cytometry, serum IgG as well as secretory sIgA antibody secretion, and cytokine expression testing to conduct a comprehensive immunogenicity assessment. Both immunogen groups can effectively stimulate the host to produce ideal humoral, mucosal, and cellular immune responses, providing a theoretical basis for subsequent functional studies, such as immunogens challenge protection and elucidation of the pathogenic mechanism of ASFV.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1200297/fullAfrican swine fever virus (ASFV)ASFV-convalescent pig serummass spectrometry identificationrecombinant protein expressionimmunogenicity assessment |
| spellingShingle | Lulu Xu Lulu Xu Lulu Xu Fei Hao Fei Hao Fei Hao Dae Gwin Jeong Rong Chen Rong Chen Yuan Gan Yuan Gan Lei Zhang Lei Zhang Minjoo Yeom Jong-Woo Lim Yanfei Yu Yanfei Yu Yun Bai Yun Bai Zhiyong Zeng Yongjie Liu Qiyan Xiong Qiyan Xiong Guoqing Shao Guoqing Shao Yuzi Wu Yuzi Wu Zhixin Feng Zhixin Feng Zhixin Feng Zhixin Feng Daesub Song Xing Xie Xing Xie Xing Xie Xing Xie Mucosal and cellular immune responses elicited by nasal and intramuscular inoculation with ASFV candidate immunogens Frontiers in Immunology African swine fever virus (ASFV) ASFV-convalescent pig serum mass spectrometry identification recombinant protein expression immunogenicity assessment |
| title | Mucosal and cellular immune responses elicited by nasal and intramuscular inoculation with ASFV candidate immunogens |
| title_full | Mucosal and cellular immune responses elicited by nasal and intramuscular inoculation with ASFV candidate immunogens |
| title_fullStr | Mucosal and cellular immune responses elicited by nasal and intramuscular inoculation with ASFV candidate immunogens |
| title_full_unstemmed | Mucosal and cellular immune responses elicited by nasal and intramuscular inoculation with ASFV candidate immunogens |
| title_short | Mucosal and cellular immune responses elicited by nasal and intramuscular inoculation with ASFV candidate immunogens |
| title_sort | mucosal and cellular immune responses elicited by nasal and intramuscular inoculation with asfv candidate immunogens |
| topic | African swine fever virus (ASFV) ASFV-convalescent pig serum mass spectrometry identification recombinant protein expression immunogenicity assessment |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1200297/full |
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