Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives
Chitosan is a stressing molecule that affects the cells walls and plasma membrane of fungi. For chitosan derivatives, the action mode is not clear. In this work, we used the yeast Ustilago maydis to study the effects of these molecules on the plasma membrane, focusing on physiologic and stress respo...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-12-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/22/12/1745 |
| _version_ | 1811233560762253312 |
|---|---|
| author | Dario Rafael Olicón-Hernández Cristina Uribe-Alvarez Salvador Uribe-Carvajal Juan Pablo Pardo Guadalupe Guerra-Sánchez |
| author_facet | Dario Rafael Olicón-Hernández Cristina Uribe-Alvarez Salvador Uribe-Carvajal Juan Pablo Pardo Guadalupe Guerra-Sánchez |
| author_sort | Dario Rafael Olicón-Hernández |
| collection | DOAJ |
| description | Chitosan is a stressing molecule that affects the cells walls and plasma membrane of fungi. For chitosan derivatives, the action mode is not clear. In this work, we used the yeast Ustilago maydis to study the effects of these molecules on the plasma membrane, focusing on physiologic and stress responses to chitosan (CH), oligochitosan (OCH), and glycol-chitosan (GCH). Yeasts were cultured with each of these molecules at 1 mg·mL−1 in minimal medium. To compare plasma membrane damage, cells were cultivated in isosmolar medium. Membrane potential (Δψ) as well as oxidative stress were measured. Changes in the total plasma membrane phospholipid and protein profiles were analyzed using standard methods, and fluorescence-stained mitochondria were observed. High osmolarity did not protect against CH inhibition and neither affected membrane potential. The OCH did produce higher oxidative stress. The effects of these molecules were evidenced by modifications in the plasma membrane protein profile. Also, mitochondrial damage was evident for CH and OCH, while GCH resulted in thicker cells with fewer mitochondria and higher glycogen accumulation. |
| first_indexed | 2024-04-12T11:21:53Z |
| format | Article |
| id | doaj.art-197c1ac818ec4f47b2adc508d1c8fa40 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T11:21:53Z |
| publishDate | 2017-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-197c1ac818ec4f47b2adc508d1c8fa402022-12-22T03:35:19ZengMDPI AGMolecules1420-30492017-12-012212174510.3390/molecules22121745molecules22121745Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin DerivativesDario Rafael Olicón-Hernández0Cristina Uribe-Alvarez1Salvador Uribe-Carvajal2Juan Pablo Pardo3Guadalupe Guerra-Sánchez4Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Departamento de Microbiología, Prolongación de Carpio y Plan de Ayala S/N, Col. Sto. Tomas, Del, Miguel Hidalgo, CP 11340 Ciudad de México, MexicoUniversidad Nacional Autónoma de México, Instituto de Fisiología Celular, Circuito exterior S/N, Ciudad Universitaria, CP 04510 Ciudad de México, MexicoUniversidad Nacional Autónoma de México, Instituto de Fisiología Celular, Circuito exterior S/N, Ciudad Universitaria, CP 04510 Ciudad de México, MexicoUniversidad Nacional Autónoma de México, Facultad de Medicina, Departamento de Bioquímica, Circuito exterior S/N, Ciudad Universitaria, CP 04510 Ciudad de México, MexicoInstituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Departamento de Microbiología, Prolongación de Carpio y Plan de Ayala S/N, Col. Sto. Tomas, Del, Miguel Hidalgo, CP 11340 Ciudad de México, MexicoChitosan is a stressing molecule that affects the cells walls and plasma membrane of fungi. For chitosan derivatives, the action mode is not clear. In this work, we used the yeast Ustilago maydis to study the effects of these molecules on the plasma membrane, focusing on physiologic and stress responses to chitosan (CH), oligochitosan (OCH), and glycol-chitosan (GCH). Yeasts were cultured with each of these molecules at 1 mg·mL−1 in minimal medium. To compare plasma membrane damage, cells were cultivated in isosmolar medium. Membrane potential (Δψ) as well as oxidative stress were measured. Changes in the total plasma membrane phospholipid and protein profiles were analyzed using standard methods, and fluorescence-stained mitochondria were observed. High osmolarity did not protect against CH inhibition and neither affected membrane potential. The OCH did produce higher oxidative stress. The effects of these molecules were evidenced by modifications in the plasma membrane protein profile. Also, mitochondrial damage was evident for CH and OCH, while GCH resulted in thicker cells with fewer mitochondria and higher glycogen accumulation.https://www.mdpi.com/1420-3049/22/12/1745chitosanoligochitosanglycol-chitosanUstilago maydisstress response |
| spellingShingle | Dario Rafael Olicón-Hernández Cristina Uribe-Alvarez Salvador Uribe-Carvajal Juan Pablo Pardo Guadalupe Guerra-Sánchez Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives Molecules chitosan oligochitosan glycol-chitosan Ustilago maydis stress response |
| title | Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives |
| title_full | Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives |
| title_fullStr | Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives |
| title_full_unstemmed | Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives |
| title_short | Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives |
| title_sort | response of ustilago maydis against the stress caused by three polycationic chitin derivatives |
| topic | chitosan oligochitosan glycol-chitosan Ustilago maydis stress response |
| url | https://www.mdpi.com/1420-3049/22/12/1745 |
| work_keys_str_mv | AT dariorafaeloliconhernandez responseofustilagomaydisagainstthestresscausedbythreepolycationicchitinderivatives AT cristinauribealvarez responseofustilagomaydisagainstthestresscausedbythreepolycationicchitinderivatives AT salvadoruribecarvajal responseofustilagomaydisagainstthestresscausedbythreepolycationicchitinderivatives AT juanpablopardo responseofustilagomaydisagainstthestresscausedbythreepolycationicchitinderivatives AT guadalupeguerrasanchez responseofustilagomaydisagainstthestresscausedbythreepolycationicchitinderivatives |