Enhancing dentin bonding quality through Acetone wet-bonding technique: a promising approach
Objective: This paper aimed to assess the impact of the acetone wet-bonding (AWB) technique on dentin bonding and to investigate its potential underlying mechanisms.Materials and Methods: Caries-free third molars were sliced, ground, etched, water-rinsed. Then the specimens were randomly allocated t...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-12-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1309503/full |
| _version_ | 1797401396338753536 |
|---|---|
| author | Shikai Zhao Zhiyi Zhu Jian Yu Chenmin Yao Miaoyang Yu Hongye Yang Cui Huang |
| author_facet | Shikai Zhao Zhiyi Zhu Jian Yu Chenmin Yao Miaoyang Yu Hongye Yang Cui Huang |
| author_sort | Shikai Zhao |
| collection | DOAJ |
| description | Objective: This paper aimed to assess the impact of the acetone wet-bonding (AWB) technique on dentin bonding and to investigate its potential underlying mechanisms.Materials and Methods: Caries-free third molars were sliced, ground, etched, water-rinsed. Then the specimens were randomly allocated to four groups according to the following pretreatments: 1. water wet-bonding (WWB); 2. ethanol wet-bonding (EWB); 3. 50% (v/v) acetone aqueous solution (50%AWB); 4. 100% acetone solution (AWB). Singlebond universal adhesive was then applied and composite buildups were constructed. The microtensile bond strength (MTBS), failure modes and interface nanoleakage were respectively evaluated after 24 h of water storage, 10,000 times of thermocycling or 1-month collagenase ageing. In situ zymography and contact angle were also investigated.Results: Acetone pretreatment preserved MTBS after thermocycling or collagenase ageing (p < 0.05) without affecting the immediate MTBS (p > 0.05). Furthermore, AWB group manifested fewer nanoleakage than WWB group. More importantly, the contact angle of the dentin surfaces decreased significantly and collagenolytic activities within the hybrid layer were suppressed in AWB group.Conclusion: This study suggested that the AWB technique was effective in enhancing the dentin bond durability by increasing the wettability of dentin surface to dental adhesives, removing residual water in the hybrid layer, improving the penetration of adhesive monomer, and inhibiting the collagenolytic activities.Clinical significance: The lifespan of adhesive restorations would be increased by utilization of acetone wet-bonding technique. |
| first_indexed | 2024-03-09T02:09:26Z |
| format | Article |
| id | doaj.art-197f9ee8d5c446819fe071bca6113c6c |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-09T02:09:26Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-197f9ee8d5c446819fe071bca6113c6c2023-12-07T13:40:12ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-12-011110.3389/fbioe.2023.13095031309503Enhancing dentin bonding quality through Acetone wet-bonding technique: a promising approachShikai Zhao0Zhiyi Zhu1Jian Yu2Chenmin Yao3Miaoyang Yu4Hongye Yang5Cui Huang6State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School and Hospital of Stomatology, Wuhan University, Wuhan, ChinaDepartment of Stomatology, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School and Hospital of Stomatology, Wuhan University, Wuhan, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School and Hospital of Stomatology, Wuhan University, Wuhan, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School and Hospital of Stomatology, Wuhan University, Wuhan, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School and Hospital of Stomatology, Wuhan University, Wuhan, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School and Hospital of Stomatology, Wuhan University, Wuhan, ChinaObjective: This paper aimed to assess the impact of the acetone wet-bonding (AWB) technique on dentin bonding and to investigate its potential underlying mechanisms.Materials and Methods: Caries-free third molars were sliced, ground, etched, water-rinsed. Then the specimens were randomly allocated to four groups according to the following pretreatments: 1. water wet-bonding (WWB); 2. ethanol wet-bonding (EWB); 3. 50% (v/v) acetone aqueous solution (50%AWB); 4. 100% acetone solution (AWB). Singlebond universal adhesive was then applied and composite buildups were constructed. The microtensile bond strength (MTBS), failure modes and interface nanoleakage were respectively evaluated after 24 h of water storage, 10,000 times of thermocycling or 1-month collagenase ageing. In situ zymography and contact angle were also investigated.Results: Acetone pretreatment preserved MTBS after thermocycling or collagenase ageing (p < 0.05) without affecting the immediate MTBS (p > 0.05). Furthermore, AWB group manifested fewer nanoleakage than WWB group. More importantly, the contact angle of the dentin surfaces decreased significantly and collagenolytic activities within the hybrid layer were suppressed in AWB group.Conclusion: This study suggested that the AWB technique was effective in enhancing the dentin bond durability by increasing the wettability of dentin surface to dental adhesives, removing residual water in the hybrid layer, improving the penetration of adhesive monomer, and inhibiting the collagenolytic activities.Clinical significance: The lifespan of adhesive restorations would be increased by utilization of acetone wet-bonding technique.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1309503/fulldentinbondingadhesiveinterfaceAcetone |
| spellingShingle | Shikai Zhao Zhiyi Zhu Jian Yu Chenmin Yao Miaoyang Yu Hongye Yang Cui Huang Enhancing dentin bonding quality through Acetone wet-bonding technique: a promising approach Frontiers in Bioengineering and Biotechnology dentin bonding adhesive interface Acetone |
| title | Enhancing dentin bonding quality through Acetone wet-bonding technique: a promising approach |
| title_full | Enhancing dentin bonding quality through Acetone wet-bonding technique: a promising approach |
| title_fullStr | Enhancing dentin bonding quality through Acetone wet-bonding technique: a promising approach |
| title_full_unstemmed | Enhancing dentin bonding quality through Acetone wet-bonding technique: a promising approach |
| title_short | Enhancing dentin bonding quality through Acetone wet-bonding technique: a promising approach |
| title_sort | enhancing dentin bonding quality through acetone wet bonding technique a promising approach |
| topic | dentin bonding adhesive interface Acetone |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1309503/full |
| work_keys_str_mv | AT shikaizhao enhancingdentinbondingqualitythroughacetonewetbondingtechniqueapromisingapproach AT zhiyizhu enhancingdentinbondingqualitythroughacetonewetbondingtechniqueapromisingapproach AT jianyu enhancingdentinbondingqualitythroughacetonewetbondingtechniqueapromisingapproach AT chenminyao enhancingdentinbondingqualitythroughacetonewetbondingtechniqueapromisingapproach AT miaoyangyu enhancingdentinbondingqualitythroughacetonewetbondingtechniqueapromisingapproach AT hongyeyang enhancingdentinbondingqualitythroughacetonewetbondingtechniqueapromisingapproach AT cuihuang enhancingdentinbondingqualitythroughacetonewetbondingtechniqueapromisingapproach |