Suppression of transmembrane sodium currents on the freshly isolated hippocampal neuron cell with continuous infrared light
Physiotherapeutic effects of infrared lasers have been proved in clinic. These infrared-based regulations of the bioelectrical activities can roughly be classified into enhancement and suppression of action potential (AP), which are described by sodium (Na) and potassium (K) transmembrane current eq...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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World Scientific Publishing
2023-03-01
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| Series: | Journal of Innovative Optical Health Sciences |
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| Online Access: | https://www.worldscientific.com/doi/10.1142/S1793545822440023 |
| _version_ | 1797865814501621760 |
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| author | Fanyi Kong Xinyu Li Ruonan Jiao Kun Liu Xue Han Changkai Sun Changsen Sun |
| author_facet | Fanyi Kong Xinyu Li Ruonan Jiao Kun Liu Xue Han Changkai Sun Changsen Sun |
| author_sort | Fanyi Kong |
| collection | DOAJ |
| description | Physiotherapeutic effects of infrared lasers have been proved in clinic. These infrared-based regulations of the bioelectrical activities can roughly be classified into enhancement and suppression of action potential (AP), which are described by sodium (Na) and potassium (K) transmembrane current equations, named as Hodgkin and Huxley (HH)-model. The enhancement effect is able to evoke or strengthen the AP when infrared light is applied. Its corresponding mechanism is commonly ascribed to the changes of the cell membrane capacitance, which is transiently increased in response to the infrared radiation. The distinctive feature of the suppression effect is to inhibit or reduce the AP by the designed protocols of infrared radiation. However, its mechanism presents more complexity than that in enhancement cases. HH-model describes how the Na current determines the initial phase of AP. So, the enhancement and suppression of AP can be also ascribed to the regulations of the corresponding Na currents. Here, a continuous infrared light at the wavelength of 980[Formula: see text]nm (CIS-980) was employed to stimulate a freshly isolated hippocampal neuron in vitro and a suppression effect on the Na currents of the neuron cell was observed. Both Na and K currents, which are named as whole cell currents, were simultaneously recorded with the cell membrane capacitance current by using a patch clamp combined with infrared irradiation. The results demonstrated that the CIS-980 was able to reversibly increase the capacitance currents, completely suppressed Na currents, but little changed K currents, which forms the steady outward whole cell currents and plays a major role on the AP repolarization. A confirmation experiment was designed and carried out by synchronizing tens of milliseconds of infrared stimulation on the same kinds of hippocampal neuron cells. After the blocked K channel, a reduction of Na current amplitude was still recorded. This proved that infrared suppression of Na current was irrelevant to K channel. A membrane capacitance mediation process was preliminarily proposed to explain the Na channel suppression process. |
| first_indexed | 2024-04-09T23:14:57Z |
| format | Article |
| id | doaj.art-197fb90135a44f369dddf45b3657d0d2 |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-04-09T23:14:57Z |
| publishDate | 2023-03-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-197fb90135a44f369dddf45b3657d0d22023-03-22T10:03:09ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052023-03-01160210.1142/S1793545822440023Suppression of transmembrane sodium currents on the freshly isolated hippocampal neuron cell with continuous infrared lightFanyi Kong0Xinyu Li1Ruonan Jiao2Kun Liu3Xue Han4Changkai Sun5Changsen Sun6School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, No. 2 Linggong Road, High-tech Zone, Dalian 116024, P. R. ChinaSchool of Electronics and Information Technology, Yat-sen University, No. 135 Xingang Xi Road, Guangzhou 510006, P. R. ChinaSchool of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, No. 2 Linggong Road, High-tech Zone, Dalian 116024, P. R. ChinaSchool of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, No. 2 Linggong Road, High-tech Zone, Dalian 116024, P. R. ChinaSchool of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, No. 2 Linggong Road, High-tech Zone, Dalian 116024, P. R. ChinaSchool of Biomedical Engineering, Dalian University of Technology, No. 2 Linggong Road, High-Tech Zone, Dalian 116024, P. R. ChinaSchool of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, No. 2 Linggong Road, High-tech Zone, Dalian 116024, P. R. ChinaPhysiotherapeutic effects of infrared lasers have been proved in clinic. These infrared-based regulations of the bioelectrical activities can roughly be classified into enhancement and suppression of action potential (AP), which are described by sodium (Na) and potassium (K) transmembrane current equations, named as Hodgkin and Huxley (HH)-model. The enhancement effect is able to evoke or strengthen the AP when infrared light is applied. Its corresponding mechanism is commonly ascribed to the changes of the cell membrane capacitance, which is transiently increased in response to the infrared radiation. The distinctive feature of the suppression effect is to inhibit or reduce the AP by the designed protocols of infrared radiation. However, its mechanism presents more complexity than that in enhancement cases. HH-model describes how the Na current determines the initial phase of AP. So, the enhancement and suppression of AP can be also ascribed to the regulations of the corresponding Na currents. Here, a continuous infrared light at the wavelength of 980[Formula: see text]nm (CIS-980) was employed to stimulate a freshly isolated hippocampal neuron in vitro and a suppression effect on the Na currents of the neuron cell was observed. Both Na and K currents, which are named as whole cell currents, were simultaneously recorded with the cell membrane capacitance current by using a patch clamp combined with infrared irradiation. The results demonstrated that the CIS-980 was able to reversibly increase the capacitance currents, completely suppressed Na currents, but little changed K currents, which forms the steady outward whole cell currents and plays a major role on the AP repolarization. A confirmation experiment was designed and carried out by synchronizing tens of milliseconds of infrared stimulation on the same kinds of hippocampal neuron cells. After the blocked K channel, a reduction of Na current amplitude was still recorded. This proved that infrared suppression of Na current was irrelevant to K channel. A membrane capacitance mediation process was preliminarily proposed to explain the Na channel suppression process.https://www.worldscientific.com/doi/10.1142/S1793545822440023Na channel suppressionAPwhole cell currentsinfrared suppression of bioelectrical activityphotothermal effect on the membrane capacitancecontinuous infrared laser physiotherapy |
| spellingShingle | Fanyi Kong Xinyu Li Ruonan Jiao Kun Liu Xue Han Changkai Sun Changsen Sun Suppression of transmembrane sodium currents on the freshly isolated hippocampal neuron cell with continuous infrared light Journal of Innovative Optical Health Sciences Na channel suppression AP whole cell currents infrared suppression of bioelectrical activity photothermal effect on the membrane capacitance continuous infrared laser physiotherapy |
| title | Suppression of transmembrane sodium currents on the freshly isolated hippocampal neuron cell with continuous infrared light |
| title_full | Suppression of transmembrane sodium currents on the freshly isolated hippocampal neuron cell with continuous infrared light |
| title_fullStr | Suppression of transmembrane sodium currents on the freshly isolated hippocampal neuron cell with continuous infrared light |
| title_full_unstemmed | Suppression of transmembrane sodium currents on the freshly isolated hippocampal neuron cell with continuous infrared light |
| title_short | Suppression of transmembrane sodium currents on the freshly isolated hippocampal neuron cell with continuous infrared light |
| title_sort | suppression of transmembrane sodium currents on the freshly isolated hippocampal neuron cell with continuous infrared light |
| topic | Na channel suppression AP whole cell currents infrared suppression of bioelectrical activity photothermal effect on the membrane capacitance continuous infrared laser physiotherapy |
| url | https://www.worldscientific.com/doi/10.1142/S1793545822440023 |
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