PI3K/Akt Signaling Involved with Osteoinductive Effects of Achatina fulica Mucus
Abstract Mesenchymal stem cells and osteoblasts play important roles in bone formation. Achatina fulica mucus presented the property of osteoinduction. This study aimed to examine the effects of A. fulica mucus on human mesenchymal stem cell (hMSC) and human fetal osteoblastic cell line (HFOB) diffe...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2020-05-01
|
| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/pdf/babt/v63/1516-8913-babt-63-e20180501.pdf |
| _version_ | 1818752943394914304 |
|---|---|
| author | Fahsai Kantawong Thananat Jearasakwattana Alisa Nira Jumaila Chewae Phusanisa Sajjamongkol Pajaree Phothong Teerasak E-kobon Pramote Chumnanpuen |
| author_facet | Fahsai Kantawong Thananat Jearasakwattana Alisa Nira Jumaila Chewae Phusanisa Sajjamongkol Pajaree Phothong Teerasak E-kobon Pramote Chumnanpuen |
| author_sort | Fahsai Kantawong |
| collection | DOAJ |
| description | Abstract Mesenchymal stem cells and osteoblasts play important roles in bone formation. Achatina fulica mucus presented the property of osteoinduction. This study aimed to examine the effects of A. fulica mucus on human mesenchymal stem cell (hMSC) and human fetal osteoblastic cell line (HFOB) differentiation. The integrated effects of A. fulica mucus and polycaprolactone (PCL) on the differentiation of hMSCs were tested. The cell viability of hMSCs treated with A. fulica mucus was investigated by the MTT assay. The cell mineralization was observed by Alizarin Red S staining, the gene expression was investigated using RT-PCR, and the PI3K activation was studied using flow cytometry. The results indicated that A. fulica mucus induced osteogenic differentiation in hMSCs and HFOBs by upregulation of the osteogenic markers; osteopontin (OPN) and osteocalcin (OCN). The results of the Alizarin Red S staining indicated that A. fulica mucus supported mineralization in both hMSCs and HFOBs. The hMSCs cultured on PCL supplemented with A. fulica mucus showed significantly increased RUNX2 and OPN expressions. A. fulica mucus was observed to increase PI3K activation in hMSCs. The findings of this study suggested that A. fulica mucus and biomaterials could be applied together for use in bone regeneration in the future. |
| first_indexed | 2024-12-18T04:59:29Z |
| format | Article |
| id | doaj.art-1980308cdde948c382f5b1c1c4959f31 |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-18T04:59:29Z |
| publishDate | 2020-05-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-1980308cdde948c382f5b1c1c4959f312022-12-21T21:20:10ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242020-05-016310.1590/1678-4324-2020180501PI3K/Akt Signaling Involved with Osteoinductive Effects of Achatina fulica MucusFahsai Kantawonghttps://orcid.org/0000-0003-0323-3021Thananat Jearasakwattanahttps://orcid.org/0000-0001-9253-8542Alisa Nirahttps://orcid.org/0000-0002-6401-612XJumaila Chewaehttps://orcid.org/0000-0001-8799-798XPhusanisa Sajjamongkolhttps://orcid.org/0000-0003-1863-6464Pajaree Phothonghttps://orcid.org/0000-0003-4673-6948Teerasak E-kobonhttps://orcid.org/0000-0002-3919-9841Pramote Chumnanpuenhttps://orcid.org/0000-0003-3072-1733Abstract Mesenchymal stem cells and osteoblasts play important roles in bone formation. Achatina fulica mucus presented the property of osteoinduction. This study aimed to examine the effects of A. fulica mucus on human mesenchymal stem cell (hMSC) and human fetal osteoblastic cell line (HFOB) differentiation. The integrated effects of A. fulica mucus and polycaprolactone (PCL) on the differentiation of hMSCs were tested. The cell viability of hMSCs treated with A. fulica mucus was investigated by the MTT assay. The cell mineralization was observed by Alizarin Red S staining, the gene expression was investigated using RT-PCR, and the PI3K activation was studied using flow cytometry. The results indicated that A. fulica mucus induced osteogenic differentiation in hMSCs and HFOBs by upregulation of the osteogenic markers; osteopontin (OPN) and osteocalcin (OCN). The results of the Alizarin Red S staining indicated that A. fulica mucus supported mineralization in both hMSCs and HFOBs. The hMSCs cultured on PCL supplemented with A. fulica mucus showed significantly increased RUNX2 and OPN expressions. A. fulica mucus was observed to increase PI3K activation in hMSCs. The findings of this study suggested that A. fulica mucus and biomaterials could be applied together for use in bone regeneration in the future.http://www.scielo.br/pdf/babt/v63/1516-8913-babt-63-e20180501.pdfAchatina fulicapolycaprolactonePI3K/Aktglycosaminoglycan |
| spellingShingle | Fahsai Kantawong Thananat Jearasakwattana Alisa Nira Jumaila Chewae Phusanisa Sajjamongkol Pajaree Phothong Teerasak E-kobon Pramote Chumnanpuen PI3K/Akt Signaling Involved with Osteoinductive Effects of Achatina fulica Mucus Brazilian Archives of Biology and Technology Achatina fulica polycaprolactone PI3K/Akt glycosaminoglycan |
| title | PI3K/Akt Signaling Involved with Osteoinductive Effects of Achatina fulica Mucus |
| title_full | PI3K/Akt Signaling Involved with Osteoinductive Effects of Achatina fulica Mucus |
| title_fullStr | PI3K/Akt Signaling Involved with Osteoinductive Effects of Achatina fulica Mucus |
| title_full_unstemmed | PI3K/Akt Signaling Involved with Osteoinductive Effects of Achatina fulica Mucus |
| title_short | PI3K/Akt Signaling Involved with Osteoinductive Effects of Achatina fulica Mucus |
| title_sort | pi3k akt signaling involved with osteoinductive effects of achatina fulica mucus |
| topic | Achatina fulica polycaprolactone PI3K/Akt glycosaminoglycan |
| url | http://www.scielo.br/pdf/babt/v63/1516-8913-babt-63-e20180501.pdf |
| work_keys_str_mv | AT fahsaikantawong pi3kaktsignalinginvolvedwithosteoinductiveeffectsofachatinafulicamucus AT thananatjearasakwattana pi3kaktsignalinginvolvedwithosteoinductiveeffectsofachatinafulicamucus AT alisanira pi3kaktsignalinginvolvedwithosteoinductiveeffectsofachatinafulicamucus AT jumailachewae pi3kaktsignalinginvolvedwithosteoinductiveeffectsofachatinafulicamucus AT phusanisasajjamongkol pi3kaktsignalinginvolvedwithosteoinductiveeffectsofachatinafulicamucus AT pajareephothong pi3kaktsignalinginvolvedwithosteoinductiveeffectsofachatinafulicamucus AT teerasakekobon pi3kaktsignalinginvolvedwithosteoinductiveeffectsofachatinafulicamucus AT pramotechumnanpuen pi3kaktsignalinginvolvedwithosteoinductiveeffectsofachatinafulicamucus |