Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC
The content and composition of dietary supplements is of great interest due to their increasing consumption and variety of available brand offered in the market. Accurate determination of vitamins is important for the improvement of dietary supplement quality and nutrition assessments. In this regar...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/22/6982 |
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| author | Anca Becze Vanda Liliana Babalau Fuss Daniela Alexandra Scurtu Maria Tomoaia-Cotisel Aurora Mocanu Oana Cadar |
| author_facet | Anca Becze Vanda Liliana Babalau Fuss Daniela Alexandra Scurtu Maria Tomoaia-Cotisel Aurora Mocanu Oana Cadar |
| author_sort | Anca Becze |
| collection | DOAJ |
| description | The content and composition of dietary supplements is of great interest due to their increasing consumption and variety of available brand offered in the market. Accurate determination of vitamins is important for the improvement of dietary supplement quality and nutrition assessments. In this regard, the simultaneous determination of vitamin D3 (calcitriol—CT and cholecalciferol—CHL) and K2 (menaquinone-4—MK-4 and menaquinone-7—MK-7) in dietary supplements was developed by using ultra-high-pressure liquid chromatography (UHPLC). The overall runtime per sample was above 35 min, with the retention times of 2.40, 6.59, 7.06, and 32.6 min for vitamin D3 (CT and CHL) and vitamin K2 (MK-4 and MK-7), respectively. The limits of detection and limits of quantification for the target nutritional compounds ranged between 0.04–0.05 µg/mL, respectively. The validation results indicated that the method had reasonable linearity (<i>R</i><sup>2</sup> ≥ 0.9990), good recovery (>82%), satisfactory intra-day precision (≤1.9%) and inter-day precision (≤3.5%), and high selectivity and specificity. The validated UHPLC method was demonstrated to be precise, accurate, and robust for the simultaneous determination of vitamins D3 (CT and CHL) and K2 (MK-4 and MK-7) in dietary supplements. |
| first_indexed | 2024-03-10T05:13:29Z |
| format | Article |
| id | doaj.art-1983501203e648f09671090a9edd00b9 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:13:29Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1983501203e648f09671090a9edd00b92023-11-23T00:36:46ZengMDPI AGMolecules1420-30492021-11-012622698210.3390/molecules26226982Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLCAnca Becze0Vanda Liliana Babalau Fuss1Daniela Alexandra Scurtu2Maria Tomoaia-Cotisel3Aurora Mocanu4Oana Cadar5Research Institute for Analytical Instrumentation, National Institute of Research and Development for Optoelectronics INOE 2000, 67 Donath Street, 400293 Cluj-Napoca, RomaniaResearch Institute for Analytical Instrumentation, National Institute of Research and Development for Optoelectronics INOE 2000, 67 Donath Street, 400293 Cluj-Napoca, RomaniaResearch Institute for Analytical Instrumentation, National Institute of Research and Development for Optoelectronics INOE 2000, 67 Donath Street, 400293 Cluj-Napoca, RomaniaFaculty of Chemistry and Chemical Engineering, Research Center of Physical Chemistry, Babeş-Bolyai University, 11 Arany Janos Street, 400028 Cluj-Napoca, RomaniaFaculty of Chemistry and Chemical Engineering, Research Center of Physical Chemistry, Babeş-Bolyai University, 11 Arany Janos Street, 400028 Cluj-Napoca, RomaniaResearch Institute for Analytical Instrumentation, National Institute of Research and Development for Optoelectronics INOE 2000, 67 Donath Street, 400293 Cluj-Napoca, RomaniaThe content and composition of dietary supplements is of great interest due to their increasing consumption and variety of available brand offered in the market. Accurate determination of vitamins is important for the improvement of dietary supplement quality and nutrition assessments. In this regard, the simultaneous determination of vitamin D3 (calcitriol—CT and cholecalciferol—CHL) and K2 (menaquinone-4—MK-4 and menaquinone-7—MK-7) in dietary supplements was developed by using ultra-high-pressure liquid chromatography (UHPLC). The overall runtime per sample was above 35 min, with the retention times of 2.40, 6.59, 7.06, and 32.6 min for vitamin D3 (CT and CHL) and vitamin K2 (MK-4 and MK-7), respectively. The limits of detection and limits of quantification for the target nutritional compounds ranged between 0.04–0.05 µg/mL, respectively. The validation results indicated that the method had reasonable linearity (<i>R</i><sup>2</sup> ≥ 0.9990), good recovery (>82%), satisfactory intra-day precision (≤1.9%) and inter-day precision (≤3.5%), and high selectivity and specificity. The validated UHPLC method was demonstrated to be precise, accurate, and robust for the simultaneous determination of vitamins D3 (CT and CHL) and K2 (MK-4 and MK-7) in dietary supplements.https://www.mdpi.com/1420-3049/26/22/6982UHPLCmethod validationcalcitriolcholecalciferolmenaquinone-4menaquinone-7 |
| spellingShingle | Anca Becze Vanda Liliana Babalau Fuss Daniela Alexandra Scurtu Maria Tomoaia-Cotisel Aurora Mocanu Oana Cadar Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC Molecules UHPLC method validation calcitriol cholecalciferol menaquinone-4 menaquinone-7 |
| title | Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC |
| title_full | Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC |
| title_fullStr | Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC |
| title_full_unstemmed | Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC |
| title_short | Simultaneous Determination of Vitamins D3 (Calcitriol, Cholecalciferol) and K2 (Menaquinone-4 and Menaquinone-7) in Dietary Supplements by UHPLC |
| title_sort | simultaneous determination of vitamins d3 calcitriol cholecalciferol and k2 menaquinone 4 and menaquinone 7 in dietary supplements by uhplc |
| topic | UHPLC method validation calcitriol cholecalciferol menaquinone-4 menaquinone-7 |
| url | https://www.mdpi.com/1420-3049/26/22/6982 |
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