Neuronal injury and tumor necrosis factor-alpha immunoreactivity in the rat hippocampus in the early period of asphyxia-induced cardiac arrest under normothermia
Low survival rate occurs in patients who initially experience a spontaneous return of circulation after cardiac arrest (CA). In this study, we induced asphyxial CA in adult male Sprague-Daley rats, maintained their body temperature at 37 ± 0.5°C, and then observed the survival rate during the post-r...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Neural Regeneration Research |
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| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=12;spage=2007;epage=2013;aulast=Tae |
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| author | Hyun-Jin Tae Il Jun Kang Tae-Kyeong Lee Jeong Hwi Cho Jae-Chul Lee Myoung Cheol Shin Yoon Sung Kim Jun Hwi Cho Jong-Dai Kim Ji Hyeon Ahn Joon Ha Park In-Shik Kim Hyang-Ah Lee Yang Hee Kim Moo-Ho Won Young Joo Lee |
| author_facet | Hyun-Jin Tae Il Jun Kang Tae-Kyeong Lee Jeong Hwi Cho Jae-Chul Lee Myoung Cheol Shin Yoon Sung Kim Jun Hwi Cho Jong-Dai Kim Ji Hyeon Ahn Joon Ha Park In-Shik Kim Hyang-Ah Lee Yang Hee Kim Moo-Ho Won Young Joo Lee |
| author_sort | Hyun-Jin Tae |
| collection | DOAJ |
| description | Low survival rate occurs in patients who initially experience a spontaneous return of circulation after cardiac arrest (CA). In this study, we induced asphyxial CA in adult male Sprague-Daley rats, maintained their body temperature at 37 ± 0.5°C, and then observed the survival rate during the post-resuscitation phase. We examined neuronal damage in the hippocampus using cresyl violet (CV) and Fluore-Jade B (F-J B) staining, and pro-inflammatory response using ionized calcium-binding adapter molecule 1 (Iba-1), glial fibrillary acidic protein (GFAP), and tumor necrosis factor-alpha (TNF-α) immunohistochemistry in the hippocampus after asphyxial CA in rats under normothermia. Our results show that the survival rate decreased gradually post-CA (about 63% at 6 hours, 37% at 1 day, and 8% at 2 days post-CA). Rats were sacrificed at these points in time post-CA, and no neuronal damage was found in the hippocampus until 1 day post-CA. However, some neurons in the stratum pyramidale of the CA region in the hippocampus were dead 2 days post-CA. Iba-1 immunoreactive microglia in the CA1 region did not change until 1 day post-CA, and they were activated (enlarged cell bodies with short and thicken processes) in all layers 2 days post-CA. Meanwhile, GFAP-immunoreactive astrocytes did not change significantly until 2 days post-CA. TNF-α immunoreactivity decreased significantly in neurons of the stratum pyramidale in the CA1 region 6 hours post-CA, decreased gradually until 1 day post-CA, and increased significantly again 2 days post-CA. These findings suggest that low survival rate of normothermic rats in the early period of asphyxia-induced CA is related to increased TNF-α immunoreactivity, but not to neuronal damage in the hippocampal CA1 region. |
| first_indexed | 2024-04-14T00:10:15Z |
| format | Article |
| id | doaj.art-1983f6e9e3704622a195ba4aaf560115 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-04-14T00:10:15Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-1983f6e9e3704622a195ba4aaf5601152022-12-22T02:23:23ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742017-01-0112122007201310.4103/1673-5374.221157Neuronal injury and tumor necrosis factor-alpha immunoreactivity in the rat hippocampus in the early period of asphyxia-induced cardiac arrest under normothermiaHyun-Jin TaeIl Jun KangTae-Kyeong LeeJeong Hwi ChoJae-Chul LeeMyoung Cheol ShinYoon Sung KimJun Hwi ChoJong-Dai KimJi Hyeon AhnJoon Ha ParkIn-Shik KimHyang-Ah LeeYang Hee KimMoo-Ho WonYoung Joo LeeLow survival rate occurs in patients who initially experience a spontaneous return of circulation after cardiac arrest (CA). In this study, we induced asphyxial CA in adult male Sprague-Daley rats, maintained their body temperature at 37 ± 0.5°C, and then observed the survival rate during the post-resuscitation phase. We examined neuronal damage in the hippocampus using cresyl violet (CV) and Fluore-Jade B (F-J B) staining, and pro-inflammatory response using ionized calcium-binding adapter molecule 1 (Iba-1), glial fibrillary acidic protein (GFAP), and tumor necrosis factor-alpha (TNF-α) immunohistochemistry in the hippocampus after asphyxial CA in rats under normothermia. Our results show that the survival rate decreased gradually post-CA (about 63% at 6 hours, 37% at 1 day, and 8% at 2 days post-CA). Rats were sacrificed at these points in time post-CA, and no neuronal damage was found in the hippocampus until 1 day post-CA. However, some neurons in the stratum pyramidale of the CA region in the hippocampus were dead 2 days post-CA. Iba-1 immunoreactive microglia in the CA1 region did not change until 1 day post-CA, and they were activated (enlarged cell bodies with short and thicken processes) in all layers 2 days post-CA. Meanwhile, GFAP-immunoreactive astrocytes did not change significantly until 2 days post-CA. TNF-α immunoreactivity decreased significantly in neurons of the stratum pyramidale in the CA1 region 6 hours post-CA, decreased gradually until 1 day post-CA, and increased significantly again 2 days post-CA. These findings suggest that low survival rate of normothermic rats in the early period of asphyxia-induced CA is related to increased TNF-α immunoreactivity, but not to neuronal damage in the hippocampal CA1 region.http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=12;spage=2007;epage=2013;aulast=Taenerve regeneration; post-cardiac arrest syndrome; normothermia; neuronal damage; gliosis; tumor necrosis factor-alpha; neural regeneration |
| spellingShingle | Hyun-Jin Tae Il Jun Kang Tae-Kyeong Lee Jeong Hwi Cho Jae-Chul Lee Myoung Cheol Shin Yoon Sung Kim Jun Hwi Cho Jong-Dai Kim Ji Hyeon Ahn Joon Ha Park In-Shik Kim Hyang-Ah Lee Yang Hee Kim Moo-Ho Won Young Joo Lee Neuronal injury and tumor necrosis factor-alpha immunoreactivity in the rat hippocampus in the early period of asphyxia-induced cardiac arrest under normothermia Neural Regeneration Research nerve regeneration; post-cardiac arrest syndrome; normothermia; neuronal damage; gliosis; tumor necrosis factor-alpha; neural regeneration |
| title | Neuronal injury and tumor necrosis factor-alpha immunoreactivity in the rat hippocampus in the early period of asphyxia-induced cardiac arrest under normothermia |
| title_full | Neuronal injury and tumor necrosis factor-alpha immunoreactivity in the rat hippocampus in the early period of asphyxia-induced cardiac arrest under normothermia |
| title_fullStr | Neuronal injury and tumor necrosis factor-alpha immunoreactivity in the rat hippocampus in the early period of asphyxia-induced cardiac arrest under normothermia |
| title_full_unstemmed | Neuronal injury and tumor necrosis factor-alpha immunoreactivity in the rat hippocampus in the early period of asphyxia-induced cardiac arrest under normothermia |
| title_short | Neuronal injury and tumor necrosis factor-alpha immunoreactivity in the rat hippocampus in the early period of asphyxia-induced cardiac arrest under normothermia |
| title_sort | neuronal injury and tumor necrosis factor alpha immunoreactivity in the rat hippocampus in the early period of asphyxia induced cardiac arrest under normothermia |
| topic | nerve regeneration; post-cardiac arrest syndrome; normothermia; neuronal damage; gliosis; tumor necrosis factor-alpha; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=12;spage=2007;epage=2013;aulast=Tae |
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