The role of the Yap5 transcription factor in remodeling gene expression in response to Fe bioavailability.
The budding yeast Saccharomyces cerevisiae has developed several mechanisms to avoid either the drastic consequences of iron deprivation or the toxic effects of iron excess. In this work, we analysed the global gene expression changes occurring in yeast cells undergoing iron overload. Several genes...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22616008/?tool=EBI |
| _version_ | 1818701473990574080 |
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| author | Catarina Pimentel Cristina Vicente Regina Andrade Menezes Soraia Caetano Laura Carreto Claudina Rodrigues-Pousada |
| author_facet | Catarina Pimentel Cristina Vicente Regina Andrade Menezes Soraia Caetano Laura Carreto Claudina Rodrigues-Pousada |
| author_sort | Catarina Pimentel |
| collection | DOAJ |
| description | The budding yeast Saccharomyces cerevisiae has developed several mechanisms to avoid either the drastic consequences of iron deprivation or the toxic effects of iron excess. In this work, we analysed the global gene expression changes occurring in yeast cells undergoing iron overload. Several genes directly or indirectly involved in iron homeostasis showed altered expression and the relevance of these changes are discussed. Microarray analyses were also performed to identify new targets of the iron responsive factor Yap5. Besides the iron vacuolar transporter CCC1, Yap5 also controls the expression of glutaredoxin GRX4, previously known to be involved in the regulation of Aft1 nuclear localization. Consistently, we show that in the absence of Yap5 Aft1 nuclear exclusion is slightly impaired. These studies provide further evidence that cells control iron homeostasis by using multiple pathways. |
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| format | Article |
| id | doaj.art-198447b4b45743a1b321c17e44e665a3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T15:21:24Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-198447b4b45743a1b321c17e44e665a32022-12-21T21:43:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3743410.1371/journal.pone.0037434The role of the Yap5 transcription factor in remodeling gene expression in response to Fe bioavailability.Catarina PimentelCristina VicenteRegina Andrade MenezesSoraia CaetanoLaura CarretoClaudina Rodrigues-PousadaThe budding yeast Saccharomyces cerevisiae has developed several mechanisms to avoid either the drastic consequences of iron deprivation or the toxic effects of iron excess. In this work, we analysed the global gene expression changes occurring in yeast cells undergoing iron overload. Several genes directly or indirectly involved in iron homeostasis showed altered expression and the relevance of these changes are discussed. Microarray analyses were also performed to identify new targets of the iron responsive factor Yap5. Besides the iron vacuolar transporter CCC1, Yap5 also controls the expression of glutaredoxin GRX4, previously known to be involved in the regulation of Aft1 nuclear localization. Consistently, we show that in the absence of Yap5 Aft1 nuclear exclusion is slightly impaired. These studies provide further evidence that cells control iron homeostasis by using multiple pathways.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22616008/?tool=EBI |
| spellingShingle | Catarina Pimentel Cristina Vicente Regina Andrade Menezes Soraia Caetano Laura Carreto Claudina Rodrigues-Pousada The role of the Yap5 transcription factor in remodeling gene expression in response to Fe bioavailability. PLoS ONE |
| title | The role of the Yap5 transcription factor in remodeling gene expression in response to Fe bioavailability. |
| title_full | The role of the Yap5 transcription factor in remodeling gene expression in response to Fe bioavailability. |
| title_fullStr | The role of the Yap5 transcription factor in remodeling gene expression in response to Fe bioavailability. |
| title_full_unstemmed | The role of the Yap5 transcription factor in remodeling gene expression in response to Fe bioavailability. |
| title_short | The role of the Yap5 transcription factor in remodeling gene expression in response to Fe bioavailability. |
| title_sort | role of the yap5 transcription factor in remodeling gene expression in response to fe bioavailability |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22616008/?tool=EBI |
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