Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration Capacity
Antibiotics are considered the cornerstone of modern medicine; however, currently, antibiotic resistance has become a global health issue. Antibiotics also find new uses in the treatment of other pathologies as well as cancer. The present study aimed to verify the impact of tetracycline and ampicill...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Current Oncology |
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| Online Access: | https://www.mdpi.com/1718-7729/28/4/225 |
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| author | Emil-Florin Hut Matilda Radulescu Nicolae Pilut Ioana Macasoi Delia Berceanu Dorina Coricovac Iulia Pinzaru Octavian Cretu Cristina Dehelean |
| author_facet | Emil-Florin Hut Matilda Radulescu Nicolae Pilut Ioana Macasoi Delia Berceanu Dorina Coricovac Iulia Pinzaru Octavian Cretu Cristina Dehelean |
| author_sort | Emil-Florin Hut |
| collection | DOAJ |
| description | Antibiotics are considered the cornerstone of modern medicine; however, currently, antibiotic resistance has become a global health issue. Antibiotics also find new uses in the treatment of other pathologies as well as cancer. The present study aimed to verify the impact of tetracycline and ampicillin in a colorectal adenocarcinoma cell line, HT-29. The effects of the two antibiotics on cell viability and nucleus were evaluated by the means of MTT assay and the Hoechst staining method, respectively. The irritant potential at vascular level of the chorioallantoic membrane was tested by the HET-CAM assay. Treatment of HT-29 cells with the two antibiotics determined different effects: (i) tetracycline induced a dose- and time-dependent cytotoxic effect characterized by decreased cell viability, changes in cells morphology, apoptotic features (nuclear fragmentation), and inhibition of cellular migration, whereas (ii) ampicillin exerted a biphasic response—cytotoxic at low doses and proliferative at high concentrations. In terms of effect on blood vessels, both antibiotics exerted a mild irritant effect. These results are promising and could be considered as starting point for further in vitro studies to define the molecular mechanisms involved in the cytotoxic/proliferative effects. |
| first_indexed | 2024-03-10T08:00:43Z |
| format | Article |
| id | doaj.art-198688fd10754f1e8f9be76238fd17b0 |
| institution | Directory Open Access Journal |
| issn | 1198-0052 1718-7729 |
| language | English |
| last_indexed | 2024-03-10T08:00:43Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Oncology |
| spelling | doaj.art-198688fd10754f1e8f9be76238fd17b02023-11-22T11:26:57ZengMDPI AGCurrent Oncology1198-00521718-77292021-07-012842466248010.3390/curroncol28040225Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration CapacityEmil-Florin Hut0Matilda Radulescu1Nicolae Pilut2Ioana Macasoi3Delia Berceanu4Dorina Coricovac5Iulia Pinzaru6Octavian Cretu7Cristina Dehelean8Faculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, RO-300041 Timisoara, RomaniaFaculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, RO-300041 Timisoara, RomaniaFaculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, RO-300041 Timisoara, RomaniaFaculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, RO-300041 Timisoara, RomaniaFaculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, RO-300041 Timisoara, RomaniaFaculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, RO-300041 Timisoara, RomaniaFaculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, RO-300041 Timisoara, RomaniaFaculty of Medicine, “Victor Babeș” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, RO-300041 Timisoara, RomaniaFaculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, RO-300041 Timisoara, RomaniaAntibiotics are considered the cornerstone of modern medicine; however, currently, antibiotic resistance has become a global health issue. Antibiotics also find new uses in the treatment of other pathologies as well as cancer. The present study aimed to verify the impact of tetracycline and ampicillin in a colorectal adenocarcinoma cell line, HT-29. The effects of the two antibiotics on cell viability and nucleus were evaluated by the means of MTT assay and the Hoechst staining method, respectively. The irritant potential at vascular level of the chorioallantoic membrane was tested by the HET-CAM assay. Treatment of HT-29 cells with the two antibiotics determined different effects: (i) tetracycline induced a dose- and time-dependent cytotoxic effect characterized by decreased cell viability, changes in cells morphology, apoptotic features (nuclear fragmentation), and inhibition of cellular migration, whereas (ii) ampicillin exerted a biphasic response—cytotoxic at low doses and proliferative at high concentrations. In terms of effect on blood vessels, both antibiotics exerted a mild irritant effect. These results are promising and could be considered as starting point for further in vitro studies to define the molecular mechanisms involved in the cytotoxic/proliferative effects.https://www.mdpi.com/1718-7729/28/4/225antibioticstetracyclineampicillincolorectal adenocarcinomaHET-CAM assayHoechst staining |
| spellingShingle | Emil-Florin Hut Matilda Radulescu Nicolae Pilut Ioana Macasoi Delia Berceanu Dorina Coricovac Iulia Pinzaru Octavian Cretu Cristina Dehelean Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration Capacity Current Oncology antibiotics tetracycline ampicillin colorectal adenocarcinoma HET-CAM assay Hoechst staining |
| title | Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration Capacity |
| title_full | Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration Capacity |
| title_fullStr | Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration Capacity |
| title_full_unstemmed | Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration Capacity |
| title_short | Two Antibiotics, Ampicillin and Tetracycline, Exert Different Effects in HT-29 Colorectal Adenocarcinoma Cells in Terms of Cell Viability and Migration Capacity |
| title_sort | two antibiotics ampicillin and tetracycline exert different effects in ht 29 colorectal adenocarcinoma cells in terms of cell viability and migration capacity |
| topic | antibiotics tetracycline ampicillin colorectal adenocarcinoma HET-CAM assay Hoechst staining |
| url | https://www.mdpi.com/1718-7729/28/4/225 |
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