Improvement of thermal-stability of enzyme immobilized onto mesoporous zirconia
In our previous research, the immobilization of formaldehyde dehydrogenase (FDH) onto mesoporous silica (MPS: pore size = 12.3 nm) was investigated. However, this method could not obtain the thermal stability of the enzyme. To solve this problem, FDH was immobilized on mesoporous zirconia material (...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2014-03-01
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| Series: | Journal of Asian Ceramic Societies |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S218707641300078X |
| _version_ | 1819290206601216000 |
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| author | Yuichi Masuda Shin-ichi Kugimiya Katsuya Kato |
| author_facet | Yuichi Masuda Shin-ichi Kugimiya Katsuya Kato |
| author_sort | Yuichi Masuda |
| collection | DOAJ |
| description | In our previous research, the immobilization of formaldehyde dehydrogenase (FDH) onto mesoporous silica (MPS: pore size = 12.3 nm) was investigated. However, this method could not obtain the thermal stability of the enzyme. To solve this problem, FDH was immobilized on mesoporous zirconia material (MPZ) with a lower thermal conductivity. MPZ was synthesized using [poly(ethylene glycol)–poly(propylene glycol)–poly(ethylene glycol) (Pluronic P123, EO20PO70EO20)], zirconium(IV) n-propoxide (ca. 75% in 1-propanol), acetylacetone, 1, 3, 5-trimethylbenzene, and ethanol. The material retained high surface area (113.6 m2/g) and pore volume (0.27 cm3/g). It was characterized by Brunauer–Emmett–Teller surface area, Barrett–Joyner–Halenda pore size distribution, X-ray diffraction, field-emission scanning electron microscopy, transmission electron microscopy, and energy-dispersive X-ray spectroscopy.
Thereafter, FDH immobilized on MPZ showed higher catalytic activity than that on MPS. Enhancement of catalytic activity was obtained by improving the substrate affinity derived from interparticle voids of MPZ. In addition, the FDH immobilized on MPZ had a very great higher thermal stability. Further investigation using transmittance Infrared spectroscopy indicated that the high-order structure of the FDH immobilized on MPZ did not get altered after the heat-treatment. |
| first_indexed | 2024-12-24T03:19:04Z |
| format | Article |
| id | doaj.art-1987342accb54b3682a155724248cf80 |
| institution | Directory Open Access Journal |
| issn | 2187-0764 |
| language | English |
| last_indexed | 2024-12-24T03:19:04Z |
| publishDate | 2014-03-01 |
| publisher | Taylor & Francis Group |
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| series | Journal of Asian Ceramic Societies |
| spelling | doaj.art-1987342accb54b3682a155724248cf802022-12-21T17:17:33ZengTaylor & Francis GroupJournal of Asian Ceramic Societies2187-07642014-03-0121111910.1016/j.jascer.2013.12.003Improvement of thermal-stability of enzyme immobilized onto mesoporous zirconiaYuichi Masuda0Shin-ichi Kugimiya1Katsuya Kato2Materials Chemistry Course, Graduate School of Engineering, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-cho, Toyota 470-0392, JapanMaterials Chemistry Course, Graduate School of Engineering, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-cho, Toyota 470-0392, JapanNational Institute of Advanced Industrial Science and Technology (AIST), 2266-98, Anagahora, Shimosidami, Moriyama-ku, Nagoya 463-8510, JapanIn our previous research, the immobilization of formaldehyde dehydrogenase (FDH) onto mesoporous silica (MPS: pore size = 12.3 nm) was investigated. However, this method could not obtain the thermal stability of the enzyme. To solve this problem, FDH was immobilized on mesoporous zirconia material (MPZ) with a lower thermal conductivity. MPZ was synthesized using [poly(ethylene glycol)–poly(propylene glycol)–poly(ethylene glycol) (Pluronic P123, EO20PO70EO20)], zirconium(IV) n-propoxide (ca. 75% in 1-propanol), acetylacetone, 1, 3, 5-trimethylbenzene, and ethanol. The material retained high surface area (113.6 m2/g) and pore volume (0.27 cm3/g). It was characterized by Brunauer–Emmett–Teller surface area, Barrett–Joyner–Halenda pore size distribution, X-ray diffraction, field-emission scanning electron microscopy, transmission electron microscopy, and energy-dispersive X-ray spectroscopy. Thereafter, FDH immobilized on MPZ showed higher catalytic activity than that on MPS. Enhancement of catalytic activity was obtained by improving the substrate affinity derived from interparticle voids of MPZ. In addition, the FDH immobilized on MPZ had a very great higher thermal stability. Further investigation using transmittance Infrared spectroscopy indicated that the high-order structure of the FDH immobilized on MPZ did not get altered after the heat-treatment.http://www.sciencedirect.com/science/article/pii/S218707641300078XMesoporous zirconiaEnzymeImmobilizationThermal stability |
| spellingShingle | Yuichi Masuda Shin-ichi Kugimiya Katsuya Kato Improvement of thermal-stability of enzyme immobilized onto mesoporous zirconia Journal of Asian Ceramic Societies Mesoporous zirconia Enzyme Immobilization Thermal stability |
| title | Improvement of thermal-stability of enzyme immobilized onto mesoporous zirconia |
| title_full | Improvement of thermal-stability of enzyme immobilized onto mesoporous zirconia |
| title_fullStr | Improvement of thermal-stability of enzyme immobilized onto mesoporous zirconia |
| title_full_unstemmed | Improvement of thermal-stability of enzyme immobilized onto mesoporous zirconia |
| title_short | Improvement of thermal-stability of enzyme immobilized onto mesoporous zirconia |
| title_sort | improvement of thermal stability of enzyme immobilized onto mesoporous zirconia |
| topic | Mesoporous zirconia Enzyme Immobilization Thermal stability |
| url | http://www.sciencedirect.com/science/article/pii/S218707641300078X |
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