Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children
Abstract Background Varicella-zoster virus (VZV) infection in children is an important public health problem in China. We performed the current study to explore the seroprevalence of VZV infection in Chinese children in order to provide more information for improvement of varicella vaccination in Ch...
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BMC
2019-07-01
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| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12879-019-4233-7 |
| _version_ | 1818198801567973376 |
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| author | Lin Luan Xiaochen Shen Jing Qiu Yang Jing Jingqi Zhang Jie Wang Jun Zhang Chen Dong |
| author_facet | Lin Luan Xiaochen Shen Jing Qiu Yang Jing Jingqi Zhang Jie Wang Jun Zhang Chen Dong |
| author_sort | Lin Luan |
| collection | DOAJ |
| description | Abstract Background Varicella-zoster virus (VZV) infection in children is an important public health problem in China. We performed the current study to explore the seroprevalence of VZV infection in Chinese children in order to provide more information for improvement of varicella vaccination in China. Methods Three thousand fourteen children were recruited from Chinese kindergarten students aged from four to six years. Anti-VZV IgG and IgM were assayed using enzyme-linked immunosorbent assay. Both ORF22 and ORF62 of VZV were amplified, sequenced, and analyzed by nested PCR. Results Among 3014 children, 43.9% of boys and 46.3% of girls were vaccinated with varicella vaccine, respectively. The seroprevalence of anti-VZV IgG was 54.4% in the children with varicella vaccination, which was significantly higher than those in unvaccinated children (49.2%) (χ2 = 8.206, P = 0.004). Among of the vaccinated children, the detection rates of VZV IgG antibody increased with age, with 49.4, 50.9 and 58.9% in 4, 5 and 6-year groups, respectively (Trend χ2 = 17.202, P = 0.002). However, there was no difference in anti-VZV IgG detection rates among those unvaccinated children in different age groups (Trend χ2 = 8.681, P = 0.070). In addition, 13 boys and 13 girls were positive for anti-VZV IgM, respectively. Among of them, eight children (0.6%) have received varicella vaccination, which was similar to those in unvaccinated children (1.1%). However, only one ORF22 sequence was isolated from an unvaccinated 5-year boy. Compared to the reference VZV sequences, the nucleotide homology was estimated to be 99.7% with genotype J. Conclusions Our study indicated that about half of Chinese children aged four to six years have a high risk of VZV infection. It should be helpful for the evaluation on the necessity of varicella immunization in China. |
| first_indexed | 2024-12-12T02:11:39Z |
| format | Article |
| id | doaj.art-1987bb2131ed4ff482ae3a378628516c |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-12T02:11:39Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-1987bb2131ed4ff482ae3a378628516c2022-12-22T00:41:53ZengBMCBMC Infectious Diseases1471-23342019-07-011911610.1186/s12879-019-4233-7Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese childrenLin Luan0Xiaochen Shen1Jing Qiu2Yang Jing3Jingqi Zhang4Jie Wang5Jun Zhang6Chen Dong7Suzhou Center for Disease Control and PreventionGusu Center for Disease Control and PreventionDepartment of Epidemiology and Statistics, School of Public Health, Jiangsu Key Laboratory and Translational Medicine for Geriatric Disease, Medical College of Soochow UniversityDepartment of Epidemiology and Statistics, School of Public Health, Jiangsu Key Laboratory and Translational Medicine for Geriatric Disease, Medical College of Soochow UniversityDepartment of Epidemiology and Statistics, School of Public Health, Jiangsu Key Laboratory and Translational Medicine for Geriatric Disease, Medical College of Soochow UniversityDepartment of Epidemiology and Statistics, School of Public Health, Jiangsu Key Laboratory and Translational Medicine for Geriatric Disease, Medical College of Soochow UniversitySuzhou Center for Disease Control and PreventionDepartment of Epidemiology and Statistics, School of Public Health, Jiangsu Key Laboratory and Translational Medicine for Geriatric Disease, Medical College of Soochow UniversityAbstract Background Varicella-zoster virus (VZV) infection in children is an important public health problem in China. We performed the current study to explore the seroprevalence of VZV infection in Chinese children in order to provide more information for improvement of varicella vaccination in China. Methods Three thousand fourteen children were recruited from Chinese kindergarten students aged from four to six years. Anti-VZV IgG and IgM were assayed using enzyme-linked immunosorbent assay. Both ORF22 and ORF62 of VZV were amplified, sequenced, and analyzed by nested PCR. Results Among 3014 children, 43.9% of boys and 46.3% of girls were vaccinated with varicella vaccine, respectively. The seroprevalence of anti-VZV IgG was 54.4% in the children with varicella vaccination, which was significantly higher than those in unvaccinated children (49.2%) (χ2 = 8.206, P = 0.004). Among of the vaccinated children, the detection rates of VZV IgG antibody increased with age, with 49.4, 50.9 and 58.9% in 4, 5 and 6-year groups, respectively (Trend χ2 = 17.202, P = 0.002). However, there was no difference in anti-VZV IgG detection rates among those unvaccinated children in different age groups (Trend χ2 = 8.681, P = 0.070). In addition, 13 boys and 13 girls were positive for anti-VZV IgM, respectively. Among of them, eight children (0.6%) have received varicella vaccination, which was similar to those in unvaccinated children (1.1%). However, only one ORF22 sequence was isolated from an unvaccinated 5-year boy. Compared to the reference VZV sequences, the nucleotide homology was estimated to be 99.7% with genotype J. Conclusions Our study indicated that about half of Chinese children aged four to six years have a high risk of VZV infection. It should be helpful for the evaluation on the necessity of varicella immunization in China.http://link.springer.com/article/10.1186/s12879-019-4233-7Varicella-zoster virusSeroprevalence, vaccinationGenotype |
| spellingShingle | Lin Luan Xiaochen Shen Jing Qiu Yang Jing Jingqi Zhang Jie Wang Jun Zhang Chen Dong Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children BMC Infectious Diseases Varicella-zoster virus Seroprevalence, vaccination Genotype |
| title | Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children |
| title_full | Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children |
| title_fullStr | Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children |
| title_full_unstemmed | Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children |
| title_short | Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children |
| title_sort | seroprevalence and molecular characteristics of varicella zoster virus infection in chinese children |
| topic | Varicella-zoster virus Seroprevalence, vaccination Genotype |
| url | http://link.springer.com/article/10.1186/s12879-019-4233-7 |
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