Prickle isoforms determine handedness of helical morphogenesis
Subcellular asymmetry directed by the planar cell polarity (PCP) signaling pathway orients numerous morphogenetic events in both invertebrates and vertebrates. Here, we describe a morphogenetic movement in which the intertwined socket and shaft cells of the Drosophila anterior wing margin mechanosen...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-01-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/51456 |
| _version_ | 1811181014323560448 |
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| author | Bomsoo Cho Song Song Jeffrey D Axelrod |
| author_facet | Bomsoo Cho Song Song Jeffrey D Axelrod |
| author_sort | Bomsoo Cho |
| collection | DOAJ |
| description | Subcellular asymmetry directed by the planar cell polarity (PCP) signaling pathway orients numerous morphogenetic events in both invertebrates and vertebrates. Here, we describe a morphogenetic movement in which the intertwined socket and shaft cells of the Drosophila anterior wing margin mechanosensory bristles undergo PCP-directed apical rotation, inducing twisting that results in a helical structure of defined chirality. We show that the Frizzled/Vang PCP signaling module coordinates polarity among and between bristles and surrounding cells to direct this rotation. Furthermore, we show that dynamic interplay between two isoforms of the Prickle protein determines right- or left-handed bristle morphogenesis. We provide evidence that, Frizzled/Vang signaling couples to the Fat/Dachsous PCP directional signal in opposite directions depending on whether Pkpk or Pksple predominates. Dynamic interplay between Pk isoforms is likely to be an important determinant of PCP outcomes in diverse contexts. Similar mechanisms may orient other lateralizing morphogenetic processes. |
| first_indexed | 2024-04-11T09:11:13Z |
| format | Article |
| id | doaj.art-1987f385d344420abde6f301652848b4 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:11:13Z |
| publishDate | 2020-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-1987f385d344420abde6f301652848b42022-12-22T04:32:29ZengeLife Sciences Publications LtdeLife2050-084X2020-01-01910.7554/eLife.51456Prickle isoforms determine handedness of helical morphogenesisBomsoo Cho0https://orcid.org/0000-0001-8970-9160Song Song1https://orcid.org/0000-0002-4587-8592Jeffrey D Axelrod2https://orcid.org/0000-0001-6094-7392Department of Pathology, Stanford University School of Medicine, Stanford, United StatesDepartment of Pathology, Stanford University School of Medicine, Stanford, United StatesDepartment of Pathology, Stanford University School of Medicine, Stanford, United StatesSubcellular asymmetry directed by the planar cell polarity (PCP) signaling pathway orients numerous morphogenetic events in both invertebrates and vertebrates. Here, we describe a morphogenetic movement in which the intertwined socket and shaft cells of the Drosophila anterior wing margin mechanosensory bristles undergo PCP-directed apical rotation, inducing twisting that results in a helical structure of defined chirality. We show that the Frizzled/Vang PCP signaling module coordinates polarity among and between bristles and surrounding cells to direct this rotation. Furthermore, we show that dynamic interplay between two isoforms of the Prickle protein determines right- or left-handed bristle morphogenesis. We provide evidence that, Frizzled/Vang signaling couples to the Fat/Dachsous PCP directional signal in opposite directions depending on whether Pkpk or Pksple predominates. Dynamic interplay between Pk isoforms is likely to be an important determinant of PCP outcomes in diverse contexts. Similar mechanisms may orient other lateralizing morphogenetic processes.https://elifesciences.org/articles/51456planar cell polarityPricklebristle chirality |
| spellingShingle | Bomsoo Cho Song Song Jeffrey D Axelrod Prickle isoforms determine handedness of helical morphogenesis eLife planar cell polarity Prickle bristle chirality |
| title | Prickle isoforms determine handedness of helical morphogenesis |
| title_full | Prickle isoforms determine handedness of helical morphogenesis |
| title_fullStr | Prickle isoforms determine handedness of helical morphogenesis |
| title_full_unstemmed | Prickle isoforms determine handedness of helical morphogenesis |
| title_short | Prickle isoforms determine handedness of helical morphogenesis |
| title_sort | prickle isoforms determine handedness of helical morphogenesis |
| topic | planar cell polarity Prickle bristle chirality |
| url | https://elifesciences.org/articles/51456 |
| work_keys_str_mv | AT bomsoocho prickleisoformsdeterminehandednessofhelicalmorphogenesis AT songsong prickleisoformsdeterminehandednessofhelicalmorphogenesis AT jeffreydaxelrod prickleisoformsdeterminehandednessofhelicalmorphogenesis |