Development and characterization of microsatellite markers for the tsetse species Glossina brevipalpis and preliminary population genetics analyses
Tsetse flies, the vectors of African trypanosomes are of key medical and economic importance and one of the constraints for the development of Africa. Tsetse fly control is one of the most effective and sustainable strategies used for controlling the disease. Knowledge about population structure and...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | Parasite |
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| Online Access: | https://www.parasite-journal.org/articles/parasite/full_html/2023/01/parasite230088/parasite230088.html |
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| author | Gstöttenmayer Fabian Moyaba Percy Rodriguez Montse Mulandane Fernando C. Mucache Hermógenes N. Neves Luis De Beer Chantel Ravel Sophie De Meeûs Thierry Mach Robert L. Vreysen Marc J. B. Abd-Alla Adly M.M. |
| author_facet | Gstöttenmayer Fabian Moyaba Percy Rodriguez Montse Mulandane Fernando C. Mucache Hermógenes N. Neves Luis De Beer Chantel Ravel Sophie De Meeûs Thierry Mach Robert L. Vreysen Marc J. B. Abd-Alla Adly M.M. |
| author_sort | Gstöttenmayer Fabian |
| collection | DOAJ |
| description | Tsetse flies, the vectors of African trypanosomes are of key medical and economic importance and one of the constraints for the development of Africa. Tsetse fly control is one of the most effective and sustainable strategies used for controlling the disease. Knowledge about population structure and level of gene flow between neighbouring populations of the target vector is of high importance to develop appropriate strategies for implementing effective management programmes. Microsatellites are commonly used to identify population structure and assess dispersal of the target populations and have been developed for several tsetse species but were lacking for Glossina brevipalpis. In this study, we screened the genome of G. brevipalpis to search for suitable microsatellite markers and nine were found to be efficient enough to distinguish between different tsetse populations. The availability of these novel microsatellite loci will help to better understand the population biology of G. brevipalpis and to assess the level of gene flow between different populations. Such information will help with the development of appropriate strategies to implement the sterile insect technique (SIT) in the framework of an area-wide integrated pest management (AW-IPM) approach to manage tsetse populations and ultimately address the trypanosomoses problem in these targeted areas. |
| first_indexed | 2024-03-08T10:54:59Z |
| format | Article |
| id | doaj.art-19890e5393124267ad498b4a4b68b8e9 |
| institution | Directory Open Access Journal |
| issn | 1776-1042 |
| language | English |
| last_indexed | 2024-03-08T10:54:59Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-19890e5393124267ad498b4a4b68b8e92024-01-26T16:35:25ZengEDP SciencesParasite1776-10422023-01-01303410.1051/parasite/2023038parasite230088Development and characterization of microsatellite markers for the tsetse species Glossina brevipalpis and preliminary population genetics analysesGstöttenmayer Fabian0Moyaba Percy1Rodriguez Montse2Mulandane Fernando C.3Mucache Hermógenes N.4Neves Luis5De Beer Chantel6Ravel Sophie7De Meeûs Thierry8Mach Robert L.9Vreysen Marc J. B.10Abd-Alla Adly M.M.11https://orcid.org/0000-0001-7540-4462Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna International CentreInsect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna International CentreInsect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna International CentreUniversity Eduardo Mondlane, Centro de BiotecnologiaUniversity Eduardo Mondlane, Centro de BiotecnologiaUniversity Eduardo Mondlane, Centro de BiotecnologiaInsect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna International CentreUniversity of Montpellier, Cirad, IRD, IntertrypUniversity of Montpellier, Cirad, IRD, IntertrypInstitute of Chemical, Environmental, and Bioscience Engineering, Vienna University of TechnologyInsect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna International CentreInsect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna International CentreTsetse flies, the vectors of African trypanosomes are of key medical and economic importance and one of the constraints for the development of Africa. Tsetse fly control is one of the most effective and sustainable strategies used for controlling the disease. Knowledge about population structure and level of gene flow between neighbouring populations of the target vector is of high importance to develop appropriate strategies for implementing effective management programmes. Microsatellites are commonly used to identify population structure and assess dispersal of the target populations and have been developed for several tsetse species but were lacking for Glossina brevipalpis. In this study, we screened the genome of G. brevipalpis to search for suitable microsatellite markers and nine were found to be efficient enough to distinguish between different tsetse populations. The availability of these novel microsatellite loci will help to better understand the population biology of G. brevipalpis and to assess the level of gene flow between different populations. Such information will help with the development of appropriate strategies to implement the sterile insect technique (SIT) in the framework of an area-wide integrated pest management (AW-IPM) approach to manage tsetse populations and ultimately address the trypanosomoses problem in these targeted areas.https://www.parasite-journal.org/articles/parasite/full_html/2023/01/parasite230088/parasite230088.htmlglossinatsetsemicrosatellitespopulation geneticssouth africamozambique |
| spellingShingle | Gstöttenmayer Fabian Moyaba Percy Rodriguez Montse Mulandane Fernando C. Mucache Hermógenes N. Neves Luis De Beer Chantel Ravel Sophie De Meeûs Thierry Mach Robert L. Vreysen Marc J. B. Abd-Alla Adly M.M. Development and characterization of microsatellite markers for the tsetse species Glossina brevipalpis and preliminary population genetics analyses Parasite glossina tsetse microsatellites population genetics south africa mozambique |
| title | Development and characterization of microsatellite markers for the tsetse species Glossina brevipalpis and preliminary population genetics analyses |
| title_full | Development and characterization of microsatellite markers for the tsetse species Glossina brevipalpis and preliminary population genetics analyses |
| title_fullStr | Development and characterization of microsatellite markers for the tsetse species Glossina brevipalpis and preliminary population genetics analyses |
| title_full_unstemmed | Development and characterization of microsatellite markers for the tsetse species Glossina brevipalpis and preliminary population genetics analyses |
| title_short | Development and characterization of microsatellite markers for the tsetse species Glossina brevipalpis and preliminary population genetics analyses |
| title_sort | development and characterization of microsatellite markers for the tsetse species glossina brevipalpis and preliminary population genetics analyses |
| topic | glossina tsetse microsatellites population genetics south africa mozambique |
| url | https://www.parasite-journal.org/articles/parasite/full_html/2023/01/parasite230088/parasite230088.html |
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