The Effect of Caffeine on the Myelin Repair Following Experimental Demyelination Induction in the Adult Rat Hippocampus
Multiple sclerosis is characterized by the loss of oligodendrocytes and demyelination of axons. In this study, the effect of caffeine on spatial memory in rats was investigated following demyelination induction by lysolecithin (LPC). The expression of Myelin Basic Protein (MBP), Glial Fibrillary Aci...
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| Format: | Article |
| Language: | English |
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Ferdowsi University of Mashhad
2016-07-01
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| Series: | Journal of Cell and Molecular Research |
| Subjects: | |
| Online Access: | https://jcmr.um.ac.ir/article_28683_eca48faf0287f45a1fea6faa7a174d9a.pdf |
| _version_ | 1818975604901412864 |
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| author | Shiva Khezri Neda Dasht Bozorgi Fatemeh Rahmani |
| author_facet | Shiva Khezri Neda Dasht Bozorgi Fatemeh Rahmani |
| author_sort | Shiva Khezri |
| collection | DOAJ |
| description | Multiple sclerosis is characterized by the loss of oligodendrocytes and demyelination of axons. In this study, the
effect of caffeine on spatial memory in rats was investigated following demyelination induction by lysolecithin
(LPC). The expression of Myelin Basic Protein (MBP), Glial Fibrillary Acidic Protein (GFAP), and Olig2
(oligodendrocyte lineage marker) genes was also assessed in the hippocampus. Animals were divided into seven
groups; control group: animals received normal saline by stereotaxic intrahippocampal injection in Dentate Gyrus
(DG) area; LPC group: animals received 2 μl lysolecithin by stereotaxic intrahippocampal injection in DG area (they
were evaluated 7, 14, and 28 days after LPC injection; Caffeine- treated group: animals were treated with caffeine at
doses of 30 mg/kg intraperitoneally for 7, 14, and 28 days after receiving LPC. Behavioral study was performed using
Radial Arm Maze. Moreover, the RT- PCR was carried out for gene expression investigation. The demyelination and
defective remyelination were noticeable on 28th day which suggests the demyelination decline caused by caffeine.
Behavioral study showed that on the post-lesion days, the food finding time in the LPC group was significantly higher
than that of the control group. Caffeine consumption significantly attenuated the food finding time in the treatment
compared to the LPC group. The RT-PCR analysis indicated that the lysolecithin decreased the MBP expression
especially on days 7 and 14 and conversely increased the Olig2 and GFAP expression. In addition, the caffeine
enhanced the expression of MBP compared to that of the LPC group and reduced the Olig2 and GFAP expressions.
Our results demonstrated that caffeine could increase the remyelination process in hippocampus and improve the
spatial memory following demyelination induction by the LPC. |
| first_indexed | 2024-12-20T15:58:36Z |
| format | Article |
| id | doaj.art-198ac601cec044b0bd8b21378c584f6b |
| institution | Directory Open Access Journal |
| issn | 2008-9147 2717-3364 |
| language | English |
| last_indexed | 2024-12-20T15:58:36Z |
| publishDate | 2016-07-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | Journal of Cell and Molecular Research |
| spelling | doaj.art-198ac601cec044b0bd8b21378c584f6b2022-12-21T19:34:21ZengFerdowsi University of MashhadJournal of Cell and Molecular Research2008-91472717-33642016-07-0181152410.22067/jcmr.v8i1.4933628683The Effect of Caffeine on the Myelin Repair Following Experimental Demyelination Induction in the Adult Rat HippocampusShiva Khezri0Neda Dasht Bozorgi1Fatemeh Rahmani2Urmia universityurmia universityurmia universityMultiple sclerosis is characterized by the loss of oligodendrocytes and demyelination of axons. In this study, the effect of caffeine on spatial memory in rats was investigated following demyelination induction by lysolecithin (LPC). The expression of Myelin Basic Protein (MBP), Glial Fibrillary Acidic Protein (GFAP), and Olig2 (oligodendrocyte lineage marker) genes was also assessed in the hippocampus. Animals were divided into seven groups; control group: animals received normal saline by stereotaxic intrahippocampal injection in Dentate Gyrus (DG) area; LPC group: animals received 2 μl lysolecithin by stereotaxic intrahippocampal injection in DG area (they were evaluated 7, 14, and 28 days after LPC injection; Caffeine- treated group: animals were treated with caffeine at doses of 30 mg/kg intraperitoneally for 7, 14, and 28 days after receiving LPC. Behavioral study was performed using Radial Arm Maze. Moreover, the RT- PCR was carried out for gene expression investigation. The demyelination and defective remyelination were noticeable on 28th day which suggests the demyelination decline caused by caffeine. Behavioral study showed that on the post-lesion days, the food finding time in the LPC group was significantly higher than that of the control group. Caffeine consumption significantly attenuated the food finding time in the treatment compared to the LPC group. The RT-PCR analysis indicated that the lysolecithin decreased the MBP expression especially on days 7 and 14 and conversely increased the Olig2 and GFAP expression. In addition, the caffeine enhanced the expression of MBP compared to that of the LPC group and reduced the Olig2 and GFAP expressions. Our results demonstrated that caffeine could increase the remyelination process in hippocampus and improve the spatial memory following demyelination induction by the LPC.https://jcmr.um.ac.ir/article_28683_eca48faf0287f45a1fea6faa7a174d9a.pdfdemyelinationremyelinationlysolecithinhippocampuscaffeine |
| spellingShingle | Shiva Khezri Neda Dasht Bozorgi Fatemeh Rahmani The Effect of Caffeine on the Myelin Repair Following Experimental Demyelination Induction in the Adult Rat Hippocampus Journal of Cell and Molecular Research demyelination remyelination lysolecithin hippocampus caffeine |
| title | The Effect of Caffeine on the Myelin Repair Following Experimental Demyelination Induction in the Adult Rat Hippocampus |
| title_full | The Effect of Caffeine on the Myelin Repair Following Experimental Demyelination Induction in the Adult Rat Hippocampus |
| title_fullStr | The Effect of Caffeine on the Myelin Repair Following Experimental Demyelination Induction in the Adult Rat Hippocampus |
| title_full_unstemmed | The Effect of Caffeine on the Myelin Repair Following Experimental Demyelination Induction in the Adult Rat Hippocampus |
| title_short | The Effect of Caffeine on the Myelin Repair Following Experimental Demyelination Induction in the Adult Rat Hippocampus |
| title_sort | effect of caffeine on the myelin repair following experimental demyelination induction in the adult rat hippocampus |
| topic | demyelination remyelination lysolecithin hippocampus caffeine |
| url | https://jcmr.um.ac.ir/article_28683_eca48faf0287f45a1fea6faa7a174d9a.pdf |
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