Effect of medium-chain fatty acids on growth, health, and immune response of dairy calves
ABSTRACT: It is necessary for the dairy industry to reduce calf morbidity and mortality, and the reliance on antibiotics to treat sick calves, to address the growing concern regarding antibiotic resistant bacteria. The primary objective of this study was to evaluate the effect that feeding dairy cal...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S002203022200443X |
| _version_ | 1798037360654417920 |
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| author | Rebecca N. Klopp Juan F. Hernandez Franco Harm Hogenesch Tana S. Dennis Kate E. Cowles Jacquelyn P. Boerman |
| author_facet | Rebecca N. Klopp Juan F. Hernandez Franco Harm Hogenesch Tana S. Dennis Kate E. Cowles Jacquelyn P. Boerman |
| author_sort | Rebecca N. Klopp |
| collection | DOAJ |
| description | ABSTRACT: It is necessary for the dairy industry to reduce calf morbidity and mortality, and the reliance on antibiotics to treat sick calves, to address the growing concern regarding antibiotic resistant bacteria. The primary objective of this study was to evaluate the effect that feeding dairy calves medium-chain fatty acids (MCFA) has on growth performance and health, and the secondary objective was to evaluate the effect of MCFA on energy status around weaning and the adaptive immune response following a vaccine challenge. Thirty-three Holstein bull calves (5 ± 1.6 d of age) were randomly assigned to 1 of 2 treatments. Control (CON) calves were fed milk replacer with no C8:0 or C10:0 oil added and MCFA calves were fed milk replacer with 0.5% of a combination of C8:0 or C10:0 oil added. Body weight and average daily gain were measured weekly. Feed efficiency (gain/feed) and the change in body condition score, hip width, hip height, heart girth, and paunch girth were calculated for the duration of the study. Fecal scores were recorded daily and all medical treatments were documented for the duration of the trial. On d 42, 49, and 56 of the study, a serum sample was collected from each calf and used to measure nonesterified fatty acids, β-hydroxybutyric acid, insulin, and glucose concentrations to evaluate energy status around weaning. A subset of 11 calves per treatment were enrolled in a vaccine challenge. At 21 ± 1.9 d of age (mean ± standard deviation) calves were vaccinated intramuscularly with 1 mL of endotoxin-free ovalbumin (OVA) mixed with aluminum hydroxide adjuvant. At 42 d of age (±1.9 d), blood samples were collected and used to analyze OVA-specific IgG1 and IgG2, and calves were vaccinated a second time. At 56 d of age (±1.9 d), blood samples were collected to analyze IgG1 and IgG2 as well as IFN-γ and IL-4 secreted from peripheral blood mononuclear cells (PBMC) treated with OVA or phytohemagglutinin. Data were analyzed as a completely randomized design with repeated measures when applicable. A tendency for greater daily fecal score was observed for MCFA calves compared with CON. At d 42 of the study, nonesterified fatty acid concentrations were greater in CON calves compared with MCFA. At 42 and 56 d of age, anti-OVA IgG1 concentrations for CON and MCFA calves were greater than prevaccination samples. This study suggests that feeding MCFA to calves affects the energy status of calves around weaning and vaccinating dairy calves with ovalbumin combined with an aluminum hydroxide adjuvant is an effective way to evaluate the adaptive immune responses. |
| first_indexed | 2024-04-11T21:25:31Z |
| format | Article |
| id | doaj.art-198cd23d92a045e7b8c7c0901b78c790 |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-04-11T21:25:31Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-198cd23d92a045e7b8c7c0901b78c7902022-12-22T04:02:24ZengElsevierJournal of Dairy Science0022-03022022-09-01105977387749Effect of medium-chain fatty acids on growth, health, and immune response of dairy calvesRebecca N. Klopp0Juan F. Hernandez Franco1Harm Hogenesch2Tana S. Dennis3Kate E. Cowles4Jacquelyn P. Boerman5Department of Animal Sciences, Purdue University, West Lafayette, IN 47907Department of Comparative Pathobiology, Purdue University, West Lafayette, IN 47907Department of Comparative Pathobiology, Purdue University, West Lafayette, IN 47907Nurture Research Center, Cargill Animal Nutrition, Lewisburg, OH 45338Nurture Research Center, Cargill Animal Nutrition, Lewisburg, OH 45338Department of Animal Sciences, Purdue University, West Lafayette, IN 47907; Corresponding authorABSTRACT: It is necessary for the dairy industry to reduce calf morbidity and mortality, and the reliance on antibiotics to treat sick calves, to address the growing concern regarding antibiotic resistant bacteria. The primary objective of this study was to evaluate the effect that feeding dairy calves medium-chain fatty acids (MCFA) has on growth performance and health, and the secondary objective was to evaluate the effect of MCFA on energy status around weaning and the adaptive immune response following a vaccine challenge. Thirty-three Holstein bull calves (5 ± 1.6 d of age) were randomly assigned to 1 of 2 treatments. Control (CON) calves were fed milk replacer with no C8:0 or C10:0 oil added and MCFA calves were fed milk replacer with 0.5% of a combination of C8:0 or C10:0 oil added. Body weight and average daily gain were measured weekly. Feed efficiency (gain/feed) and the change in body condition score, hip width, hip height, heart girth, and paunch girth were calculated for the duration of the study. Fecal scores were recorded daily and all medical treatments were documented for the duration of the trial. On d 42, 49, and 56 of the study, a serum sample was collected from each calf and used to measure nonesterified fatty acids, β-hydroxybutyric acid, insulin, and glucose concentrations to evaluate energy status around weaning. A subset of 11 calves per treatment were enrolled in a vaccine challenge. At 21 ± 1.9 d of age (mean ± standard deviation) calves were vaccinated intramuscularly with 1 mL of endotoxin-free ovalbumin (OVA) mixed with aluminum hydroxide adjuvant. At 42 d of age (±1.9 d), blood samples were collected and used to analyze OVA-specific IgG1 and IgG2, and calves were vaccinated a second time. At 56 d of age (±1.9 d), blood samples were collected to analyze IgG1 and IgG2 as well as IFN-γ and IL-4 secreted from peripheral blood mononuclear cells (PBMC) treated with OVA or phytohemagglutinin. Data were analyzed as a completely randomized design with repeated measures when applicable. A tendency for greater daily fecal score was observed for MCFA calves compared with CON. At d 42 of the study, nonesterified fatty acid concentrations were greater in CON calves compared with MCFA. At 42 and 56 d of age, anti-OVA IgG1 concentrations for CON and MCFA calves were greater than prevaccination samples. This study suggests that feeding MCFA to calves affects the energy status of calves around weaning and vaccinating dairy calves with ovalbumin combined with an aluminum hydroxide adjuvant is an effective way to evaluate the adaptive immune responses.http://www.sciencedirect.com/science/article/pii/S002203022200443Xmedium-chain fatty acidsgrowthhealthadaptive immune response |
| spellingShingle | Rebecca N. Klopp Juan F. Hernandez Franco Harm Hogenesch Tana S. Dennis Kate E. Cowles Jacquelyn P. Boerman Effect of medium-chain fatty acids on growth, health, and immune response of dairy calves Journal of Dairy Science medium-chain fatty acids growth health adaptive immune response |
| title | Effect of medium-chain fatty acids on growth, health, and immune response of dairy calves |
| title_full | Effect of medium-chain fatty acids on growth, health, and immune response of dairy calves |
| title_fullStr | Effect of medium-chain fatty acids on growth, health, and immune response of dairy calves |
| title_full_unstemmed | Effect of medium-chain fatty acids on growth, health, and immune response of dairy calves |
| title_short | Effect of medium-chain fatty acids on growth, health, and immune response of dairy calves |
| title_sort | effect of medium chain fatty acids on growth health and immune response of dairy calves |
| topic | medium-chain fatty acids growth health adaptive immune response |
| url | http://www.sciencedirect.com/science/article/pii/S002203022200443X |
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