Occurrence of Arbuscular Mycorrhizal Fungi in Hemp (Cannabis sativa) Plants and Soil Fertilized with Sewage Sludge and Phosphogypsum
The colonization of soil and roots by arbuscular mycorrhizal fungi and the formation of spores under the influence of fertilization with phosphogypsum and sewage sludge were examined. Identification of arbuscular mycorrhizal fungi was carried out in soil and in roots of outdoor experimental hemp pla...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-02-01
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| Series: | Journal of Natural Fibers |
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| Online Access: | http://dx.doi.org/10.1080/15440478.2019.1618779 |
| _version_ | 1797680204776210432 |
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| author | Dariusz Zielonka Lidia Sas-Paszt Edyta Derkowska Anna Lisek Stefan Russel |
| author_facet | Dariusz Zielonka Lidia Sas-Paszt Edyta Derkowska Anna Lisek Stefan Russel |
| author_sort | Dariusz Zielonka |
| collection | DOAJ |
| description | The colonization of soil and roots by arbuscular mycorrhizal fungi and the formation of spores under the influence of fertilization with phosphogypsum and sewage sludge were examined. Identification of arbuscular mycorrhizal fungi was carried out in soil and in roots of outdoor experimental hemp plantation. Assessment of the colonization was carried out by molecular and microscopic methods. The material for the isolation of DNA consisted of soil samples taken from mycorrhizal soil and hemp roots of cv. Białobrzeskie, cv. Tygra, and cv. Beniko. In nested PCR reactions part of the small subunit (SSU) region, the region of the internal transcribed spacer (ITS) and part of large subunit (LSU) rDNA region were amplified. As a result of the cloning and sequencing of the DNA, it was found that the greatest similarity of the obtained sequences present in the samples of roots and soil was to the following species: Diversispora sp., Funneliformis sp., Glomus sp., Funneliformis mosseae, Glomus caledonium, Funneliformis geosporum, Glomus occultum. The colonization by the fungi was estimated and found to differ depending on the hemp cultivar and doses of fertilizers. Cultivar Tygra showed no negative impact on biomass production by intensive mycorrhization, proved its high tolerance to phosphogypsum and sewage sludge pollution, and responded positively regarding biomass production. |
| first_indexed | 2024-03-11T23:26:19Z |
| format | Article |
| id | doaj.art-198e611493944ed4a8b6f616540ae30e |
| institution | Directory Open Access Journal |
| issn | 1544-0478 1544-046X |
| language | English |
| last_indexed | 2024-03-11T23:26:19Z |
| publishDate | 2021-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Natural Fibers |
| spelling | doaj.art-198e611493944ed4a8b6f616540ae30e2023-09-20T12:50:16ZengTaylor & Francis GroupJournal of Natural Fibers1544-04781544-046X2021-02-0118225026010.1080/15440478.2019.16187791618779Occurrence of Arbuscular Mycorrhizal Fungi in Hemp (Cannabis sativa) Plants and Soil Fertilized with Sewage Sludge and PhosphogypsumDariusz Zielonka0Lidia Sas-Paszt1Edyta Derkowska2Anna Lisek3Stefan Russel4Warsaw University of Life SciencesRhizosphere LaboratoryRhizosphere LaboratoryResearch Institute of HorticultureInstitute of Technology and Life SciencesThe colonization of soil and roots by arbuscular mycorrhizal fungi and the formation of spores under the influence of fertilization with phosphogypsum and sewage sludge were examined. Identification of arbuscular mycorrhizal fungi was carried out in soil and in roots of outdoor experimental hemp plantation. Assessment of the colonization was carried out by molecular and microscopic methods. The material for the isolation of DNA consisted of soil samples taken from mycorrhizal soil and hemp roots of cv. Białobrzeskie, cv. Tygra, and cv. Beniko. In nested PCR reactions part of the small subunit (SSU) region, the region of the internal transcribed spacer (ITS) and part of large subunit (LSU) rDNA region were amplified. As a result of the cloning and sequencing of the DNA, it was found that the greatest similarity of the obtained sequences present in the samples of roots and soil was to the following species: Diversispora sp., Funneliformis sp., Glomus sp., Funneliformis mosseae, Glomus caledonium, Funneliformis geosporum, Glomus occultum. The colonization by the fungi was estimated and found to differ depending on the hemp cultivar and doses of fertilizers. Cultivar Tygra showed no negative impact on biomass production by intensive mycorrhization, proved its high tolerance to phosphogypsum and sewage sludge pollution, and responded positively regarding biomass production.http://dx.doi.org/10.1080/15440478.2019.1618779arbuscular mycorrhizal fungibiomass productioncannabis sativahempmycorrhizal frequencysoil contamination |
| spellingShingle | Dariusz Zielonka Lidia Sas-Paszt Edyta Derkowska Anna Lisek Stefan Russel Occurrence of Arbuscular Mycorrhizal Fungi in Hemp (Cannabis sativa) Plants and Soil Fertilized with Sewage Sludge and Phosphogypsum Journal of Natural Fibers arbuscular mycorrhizal fungi biomass production cannabis sativa hemp mycorrhizal frequency soil contamination |
| title | Occurrence of Arbuscular Mycorrhizal Fungi in Hemp (Cannabis sativa) Plants and Soil Fertilized with Sewage Sludge and Phosphogypsum |
| title_full | Occurrence of Arbuscular Mycorrhizal Fungi in Hemp (Cannabis sativa) Plants and Soil Fertilized with Sewage Sludge and Phosphogypsum |
| title_fullStr | Occurrence of Arbuscular Mycorrhizal Fungi in Hemp (Cannabis sativa) Plants and Soil Fertilized with Sewage Sludge and Phosphogypsum |
| title_full_unstemmed | Occurrence of Arbuscular Mycorrhizal Fungi in Hemp (Cannabis sativa) Plants and Soil Fertilized with Sewage Sludge and Phosphogypsum |
| title_short | Occurrence of Arbuscular Mycorrhizal Fungi in Hemp (Cannabis sativa) Plants and Soil Fertilized with Sewage Sludge and Phosphogypsum |
| title_sort | occurrence of arbuscular mycorrhizal fungi in hemp cannabis sativa plants and soil fertilized with sewage sludge and phosphogypsum |
| topic | arbuscular mycorrhizal fungi biomass production cannabis sativa hemp mycorrhizal frequency soil contamination |
| url | http://dx.doi.org/10.1080/15440478.2019.1618779 |
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