Protective Effects of Nano Zinc on Barki Ram Semen Viability
Sixty Barki semen ejaculates were collected using the artificial vagina. Semen samples were evaluated, pooled then subjected for processing using different concentrations of nano zinc (10, 50, 100 and 150µg/ml). Short-term chilled storage duration, absolute survivability index, post-thaw recovery,...
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| Format: | Article |
| Language: | English |
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Assiut University
2023-06-01
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| Series: | Journal of Advanced Veterinary Research |
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| Online Access: | https://advetresearch.com/index.php/AVR/article/view/1377 |
| _version_ | 1797657030378389504 |
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| author | Mohammed R.K. Metwaly Gamal A.M. Sosa Mary G. Abdel-Malak Mohamed El-Raey Mohamed |
| author_facet | Mohammed R.K. Metwaly Gamal A.M. Sosa Mary G. Abdel-Malak Mohamed El-Raey Mohamed |
| author_sort | Mohammed R.K. Metwaly |
| collection | DOAJ |
| description |
Sixty Barki semen ejaculates were collected using the artificial vagina. Semen samples were evaluated, pooled then subjected for processing using different concentrations of nano zinc (10, 50, 100 and 150µg/ml). Short-term chilled storage duration, absolute survivability index, post-thaw recovery, reduction rate, live percent, membrane integrity, acrosomal reaction, DNA integrity, head & tail’s diameter and DNA % were assessed. 150µg/ml of nano zinc can stabilize high motility % (80±0.54%) for about 11 days post dilution and chilling, then drastically dropped on day 18 (47±0.57%). Moreover, 150µg/ml recorded the best of absolute survivability index (33096±374), motility after dilution (86.67±1.05%), after cooling (87.50 ± 1.11%), after freezing- thawing process (56.67±1.66 %). Furthermore, 150µg/ml treated group keeping the highest records along the first three hours post-thawing (54.17 ± 1.53, 50.00 ± 1.29, and 47.50 ± 1.11%, respectively). Additionally, 150 µg/ml treated samples presented the highest viability index (180±3.47%), post-thaw recovery rate (65.47±2.26%), post-thawing live % (54.17±2.58%). While 150 µg/ml treated group showed the lowest reduction % (15.81 ± 3.12 %), acrosomal reaction (9.83±0.7%). Moreover, 150µg/ml treated samples recorded the largest head diameter and highest DNA % (52.87±1.74 and 97.51±0.30%, respectively), but showed the lowest tail diameter and tail DNA % (6.22±0.33 and 2.48±0.30%%, respectively). Furthermore, 150µg/ml treated group displayed the lowest comet %, tail moment and olive tail moment (8.950±0.02 %, 0.15±0.02 and 0.40±0.10, respectively) if compared with the other nano zinc treated groups. In conclusion: 150µg/ml of ZnO-NPs improves Barki ram semen processing, particularly, chilled semen viability, motility, longevity, membrane, and DNA integrity.
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| first_indexed | 2024-03-11T17:38:30Z |
| format | Article |
| id | doaj.art-1990f1c7e5204c06a98d950bfc909c09 |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-03-11T17:38:30Z |
| publishDate | 2023-06-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-1990f1c7e5204c06a98d950bfc909c092023-10-18T14:13:46ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772023-06-01135Protective Effects of Nano Zinc on Barki Ram Semen ViabilityMohammed R.K. Metwaly0Gamal A.M. Sosa1Mary G. Abdel-Malak2Mohamed El-Raey Mohamed3Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, P.O. 13736, Tokh, Kaliobia, Egypt.Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, P.O. 13736, Tokh, Kaliobia, Egypt.Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute, Agriculture Research Center, Al Haram, P. O. 12556, Giza, Egypt.Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, P.O. 13736, Tokh, Kaliobia, Egypt. Sixty Barki semen ejaculates were collected using the artificial vagina. Semen samples were evaluated, pooled then subjected for processing using different concentrations of nano zinc (10, 50, 100 and 150µg/ml). Short-term chilled storage duration, absolute survivability index, post-thaw recovery, reduction rate, live percent, membrane integrity, acrosomal reaction, DNA integrity, head & tail’s diameter and DNA % were assessed. 150µg/ml of nano zinc can stabilize high motility % (80±0.54%) for about 11 days post dilution and chilling, then drastically dropped on day 18 (47±0.57%). Moreover, 150µg/ml recorded the best of absolute survivability index (33096±374), motility after dilution (86.67±1.05%), after cooling (87.50 ± 1.11%), after freezing- thawing process (56.67±1.66 %). Furthermore, 150µg/ml treated group keeping the highest records along the first three hours post-thawing (54.17 ± 1.53, 50.00 ± 1.29, and 47.50 ± 1.11%, respectively). Additionally, 150 µg/ml treated samples presented the highest viability index (180±3.47%), post-thaw recovery rate (65.47±2.26%), post-thawing live % (54.17±2.58%). While 150 µg/ml treated group showed the lowest reduction % (15.81 ± 3.12 %), acrosomal reaction (9.83±0.7%). Moreover, 150µg/ml treated samples recorded the largest head diameter and highest DNA % (52.87±1.74 and 97.51±0.30%, respectively), but showed the lowest tail diameter and tail DNA % (6.22±0.33 and 2.48±0.30%%, respectively). Furthermore, 150µg/ml treated group displayed the lowest comet %, tail moment and olive tail moment (8.950±0.02 %, 0.15±0.02 and 0.40±0.10, respectively) if compared with the other nano zinc treated groups. In conclusion: 150µg/ml of ZnO-NPs improves Barki ram semen processing, particularly, chilled semen viability, motility, longevity, membrane, and DNA integrity. https://advetresearch.com/index.php/AVR/article/view/1377Barki ramsSemen Cryopreservation ZnO NPsram |
| spellingShingle | Mohammed R.K. Metwaly Gamal A.M. Sosa Mary G. Abdel-Malak Mohamed El-Raey Mohamed Protective Effects of Nano Zinc on Barki Ram Semen Viability Journal of Advanced Veterinary Research Barki rams Semen Cryopreservation ZnO NPs ram |
| title | Protective Effects of Nano Zinc on Barki Ram Semen Viability |
| title_full | Protective Effects of Nano Zinc on Barki Ram Semen Viability |
| title_fullStr | Protective Effects of Nano Zinc on Barki Ram Semen Viability |
| title_full_unstemmed | Protective Effects of Nano Zinc on Barki Ram Semen Viability |
| title_short | Protective Effects of Nano Zinc on Barki Ram Semen Viability |
| title_sort | protective effects of nano zinc on barki ram semen viability |
| topic | Barki rams Semen Cryopreservation ZnO NPs ram |
| url | https://advetresearch.com/index.php/AVR/article/view/1377 |
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