Clinical Phenotypes of Patients with Anti-DFS70/LEDGF Antibodies in a Routine ANA Referral Cohort
Objective. To analyze the clinical value of anti-DFS70 antibodies in a cohort of patients undergoing routine antinuclear antibodies (ANAs) testing. Methods. Sera with a dense fine speckled (DFS) indirect immunofluorescence (IIF) pattern from 100 consecutive patients and 100 patients with other IIF p...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/703759 |
| _version_ | 1827007811428548608 |
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| author | Makoto Miyara Roger Albesa Jean-Luc Charuel Mohamed El Amri Marvin J. Fritzler Pascale Ghillani-Dalbin Zahir Amoura Lucile Musset Michael Mahler |
| author_facet | Makoto Miyara Roger Albesa Jean-Luc Charuel Mohamed El Amri Marvin J. Fritzler Pascale Ghillani-Dalbin Zahir Amoura Lucile Musset Michael Mahler |
| author_sort | Makoto Miyara |
| collection | DOAJ |
| description | Objective. To analyze the clinical value of anti-DFS70 antibodies in a cohort of patients undergoing routine antinuclear antibodies (ANAs) testing. Methods. Sera with a dense fine speckled (DFS) indirect immunofluorescence (IIF) pattern from 100 consecutive patients and 100 patients with other IIF patterns were tested for anti-DFS70 antibodies by a novel chemiluminescence immunoassay (CIA) and for ANA by ANA Screen ELISA (both INOVA). Results. Among the 100 patients with a DFS IIF pattern, 91% were anti-DFS70 positive by CIA compared to 3% in the comparator group (P<0.0001). The CIA and IIF titers of anti-DFS antibodies were highly correlated (rho = 0.89). ANA by ELISA was positive in 35% of patients with the DFS IIF pattern as compared to 67% of patients with other patterns (P<0.0001). Only 12.0% of patients with DFS pattern and 13.4% with DFS pattern and anti-DFS70 antibodies detected by CIA had systemic autoimmune rheumatic disease (SARD). Only 5/91 (5.5%) patients with anti-DFS70 antibodies had SARD and their sera were negative on the ANA Screen ELISA. Conclusion. Although anti-DFS70 antibodies cannot exclude the presence of SARD, the likelihood is significantly lower than in patients with other IIF patterns and should be included in test algorithms for ANA testing. |
| first_indexed | 2025-02-18T12:24:01Z |
| format | Article |
| id | doaj.art-1994ba897578401bbb243ed795a51dad |
| institution | Directory Open Access Journal |
| issn | 1740-2522 1740-2530 |
| language | English |
| last_indexed | 2025-02-18T12:24:01Z |
| publishDate | 2013-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj.art-1994ba897578401bbb243ed795a51dad2024-11-02T04:25:43ZengHindawi LimitedClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/703759703759Clinical Phenotypes of Patients with Anti-DFS70/LEDGF Antibodies in a Routine ANA Referral CohortMakoto Miyara0Roger Albesa1Jean-Luc Charuel2Mohamed El Amri3Marvin J. Fritzler4Pascale Ghillani-Dalbin5Zahir Amoura6Lucile Musset7Michael Mahler8Department of Immunology, (AP-HP) Pitié-Salpêtrière Hospital, Paris, FranceDepartment of Research, INOVA Diagnostics Inc., 9900 Old Grove Road, San Diego, CA 32131-1638, USADepartment of Immunology, (AP-HP) Pitié-Salpêtrière Hospital, Paris, FranceDepartment of Immunology, (AP-HP) Pitié-Salpêtrière Hospital, Paris, FranceFaculty of Medicine, University of Calgary, Calgary, CanadaDepartment of Immunology, (AP-HP) Pitié-Salpêtrière Hospital, Paris, FranceDepartment of Internal Medicine, (AP-HP) Pitié-Salpêtrière Hospital, Paris, FranceDepartment of Immunology, (AP-HP) Pitié-Salpêtrière Hospital, Paris, FranceDepartment of Research, INOVA Diagnostics Inc., 9900 Old Grove Road, San Diego, CA 32131-1638, USAObjective. To analyze the clinical value of anti-DFS70 antibodies in a cohort of patients undergoing routine antinuclear antibodies (ANAs) testing. Methods. Sera with a dense fine speckled (DFS) indirect immunofluorescence (IIF) pattern from 100 consecutive patients and 100 patients with other IIF patterns were tested for anti-DFS70 antibodies by a novel chemiluminescence immunoassay (CIA) and for ANA by ANA Screen ELISA (both INOVA). Results. Among the 100 patients with a DFS IIF pattern, 91% were anti-DFS70 positive by CIA compared to 3% in the comparator group (P<0.0001). The CIA and IIF titers of anti-DFS antibodies were highly correlated (rho = 0.89). ANA by ELISA was positive in 35% of patients with the DFS IIF pattern as compared to 67% of patients with other patterns (P<0.0001). Only 12.0% of patients with DFS pattern and 13.4% with DFS pattern and anti-DFS70 antibodies detected by CIA had systemic autoimmune rheumatic disease (SARD). Only 5/91 (5.5%) patients with anti-DFS70 antibodies had SARD and their sera were negative on the ANA Screen ELISA. Conclusion. Although anti-DFS70 antibodies cannot exclude the presence of SARD, the likelihood is significantly lower than in patients with other IIF patterns and should be included in test algorithms for ANA testing.http://dx.doi.org/10.1155/2013/703759 |
| spellingShingle | Makoto Miyara Roger Albesa Jean-Luc Charuel Mohamed El Amri Marvin J. Fritzler Pascale Ghillani-Dalbin Zahir Amoura Lucile Musset Michael Mahler Clinical Phenotypes of Patients with Anti-DFS70/LEDGF Antibodies in a Routine ANA Referral Cohort Clinical and Developmental Immunology |
| title | Clinical Phenotypes of Patients with Anti-DFS70/LEDGF Antibodies in a Routine ANA Referral Cohort |
| title_full | Clinical Phenotypes of Patients with Anti-DFS70/LEDGF Antibodies in a Routine ANA Referral Cohort |
| title_fullStr | Clinical Phenotypes of Patients with Anti-DFS70/LEDGF Antibodies in a Routine ANA Referral Cohort |
| title_full_unstemmed | Clinical Phenotypes of Patients with Anti-DFS70/LEDGF Antibodies in a Routine ANA Referral Cohort |
| title_short | Clinical Phenotypes of Patients with Anti-DFS70/LEDGF Antibodies in a Routine ANA Referral Cohort |
| title_sort | clinical phenotypes of patients with anti dfs70 ledgf antibodies in a routine ana referral cohort |
| url | http://dx.doi.org/10.1155/2013/703759 |
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