A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization
Highly sensitive (high SBR) and highly specific (high SNP discrimination ratio) DNA hybridization is essential for a biosensor with clinical application. Herein, we propose a method that allows detecting multiple pathogens on a single platform with the SNP discrimination ratios over 160:1 in the dyn...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/11/8/265 |
| _version_ | 1797524488204582912 |
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| author | Satish Balasaheb Nimse Keum-Soo Song Shrikant Dashrath Warkad Taisun Kim |
| author_facet | Satish Balasaheb Nimse Keum-Soo Song Shrikant Dashrath Warkad Taisun Kim |
| author_sort | Satish Balasaheb Nimse |
| collection | DOAJ |
| description | Highly sensitive (high SBR) and highly specific (high SNP discrimination ratio) DNA hybridization is essential for a biosensor with clinical application. Herein, we propose a method that allows detecting multiple pathogens on a single platform with the SNP discrimination ratios over 160:1 in the dynamic range of 10<sup>1</sup> to 10<sup>4</sup> copies per test. The newly developed SWAT method allows achieving highly sensitive and highly specific DNA hybridizations. The detection and discrimination of the MTB and NTM strain in the clinical samples with the SBR and SNP discrimination ratios higher than 160:1 indicate the high clinical applicability of the SWAT. |
| first_indexed | 2024-03-10T08:58:07Z |
| format | Article |
| id | doaj.art-19951f1a5244452b83b2dfe8f20c1dcd |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T08:58:07Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-19951f1a5244452b83b2dfe8f20c1dcd2023-11-22T06:57:34ZengMDPI AGBiosensors2079-63742021-08-0111826510.3390/bios11080265A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA HybridizationSatish Balasaheb Nimse0Keum-Soo Song1Shrikant Dashrath Warkad2Taisun Kim3Department of Chemistry, Institute for Applied Chemistry, Hallym University, Chuncheon 200-702, KoreaBiometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, KoreaBiometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, KoreaDepartment of Chemistry, Institute for Applied Chemistry, Hallym University, Chuncheon 200-702, KoreaHighly sensitive (high SBR) and highly specific (high SNP discrimination ratio) DNA hybridization is essential for a biosensor with clinical application. Herein, we propose a method that allows detecting multiple pathogens on a single platform with the SNP discrimination ratios over 160:1 in the dynamic range of 10<sup>1</sup> to 10<sup>4</sup> copies per test. The newly developed SWAT method allows achieving highly sensitive and highly specific DNA hybridizations. The detection and discrimination of the MTB and NTM strain in the clinical samples with the SBR and SNP discrimination ratios higher than 160:1 indicate the high clinical applicability of the SWAT.https://www.mdpi.com/2079-6374/11/8/265biosensorstuberculosisDNA-DNA hybridizationsingle nucleotide polymorphismsignal to background ratio |
| spellingShingle | Satish Balasaheb Nimse Keum-Soo Song Shrikant Dashrath Warkad Taisun Kim A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization Biosensors biosensors tuberculosis DNA-DNA hybridization single nucleotide polymorphism signal to background ratio |
| title | A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization |
| title_full | A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization |
| title_fullStr | A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization |
| title_full_unstemmed | A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization |
| title_short | A Novel Method That Allows SNP Discrimination with 160:1 Ratio for Biosensors Based on DNA-DNA Hybridization |
| title_sort | novel method that allows snp discrimination with 160 1 ratio for biosensors based on dna dna hybridization |
| topic | biosensors tuberculosis DNA-DNA hybridization single nucleotide polymorphism signal to background ratio |
| url | https://www.mdpi.com/2079-6374/11/8/265 |
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