Conformational motions and ligand-binding underlying gating and regulation in IP3R channel
Here authors report cryo-EM structures of IP3R1 which provide atomic details of IP3, Ca2+ and ATP binding. Molecular motions of key domains and sidechains were found to regulate ligand binding and gating, which are validated by functional assays.
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-34574-1 |
| _version_ | 1811319569853186048 |
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| author | Guizhen Fan Mariah R. Baker Lara E. Terry Vikas Arige Muyuan Chen Alexander B. Seryshev Matthew L. Baker Steven J. Ludtke David I. Yule Irina I. Serysheva |
| author_facet | Guizhen Fan Mariah R. Baker Lara E. Terry Vikas Arige Muyuan Chen Alexander B. Seryshev Matthew L. Baker Steven J. Ludtke David I. Yule Irina I. Serysheva |
| author_sort | Guizhen Fan |
| collection | DOAJ |
| description | Here authors report cryo-EM structures of IP3R1 which provide atomic details of IP3, Ca2+ and ATP binding. Molecular motions of key domains and sidechains were found to regulate ligand binding and gating, which are validated by functional assays. |
| first_indexed | 2024-04-13T12:45:15Z |
| format | Article |
| id | doaj.art-19978619640c40e4bd70b573581c08b9 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-13T12:45:15Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-19978619640c40e4bd70b573581c08b92022-12-22T02:46:23ZengNature PortfolioNature Communications2041-17232022-11-0113111510.1038/s41467-022-34574-1Conformational motions and ligand-binding underlying gating and regulation in IP3R channelGuizhen Fan0Mariah R. Baker1Lara E. Terry2Vikas Arige3Muyuan Chen4Alexander B. Seryshev5Matthew L. Baker6Steven J. Ludtke7David I. Yule8Irina I. Serysheva9Department of Biochemistry and Molecular Biology, Structural Biology Imaging Center, McGovern Medical School at The University of Texas Health Science Center at HoustonDepartment of Biochemistry and Molecular Biology, Structural Biology Imaging Center, McGovern Medical School at The University of Texas Health Science Center at HoustonDepartment of Pharmacology and Physiology, School of Medicine and Dentistry, University of RochesterDepartment of Pharmacology and Physiology, School of Medicine and Dentistry, University of RochesterVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of MedicineDepartment of Biochemistry and Molecular Biology, Structural Biology Imaging Center, McGovern Medical School at The University of Texas Health Science Center at HoustonDepartment of Biochemistry and Molecular Biology, Structural Biology Imaging Center, McGovern Medical School at The University of Texas Health Science Center at HoustonVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of MedicineDepartment of Pharmacology and Physiology, School of Medicine and Dentistry, University of RochesterDepartment of Biochemistry and Molecular Biology, Structural Biology Imaging Center, McGovern Medical School at The University of Texas Health Science Center at HoustonHere authors report cryo-EM structures of IP3R1 which provide atomic details of IP3, Ca2+ and ATP binding. Molecular motions of key domains and sidechains were found to regulate ligand binding and gating, which are validated by functional assays.https://doi.org/10.1038/s41467-022-34574-1 |
| spellingShingle | Guizhen Fan Mariah R. Baker Lara E. Terry Vikas Arige Muyuan Chen Alexander B. Seryshev Matthew L. Baker Steven J. Ludtke David I. Yule Irina I. Serysheva Conformational motions and ligand-binding underlying gating and regulation in IP3R channel Nature Communications |
| title | Conformational motions and ligand-binding underlying gating and regulation in IP3R channel |
| title_full | Conformational motions and ligand-binding underlying gating and regulation in IP3R channel |
| title_fullStr | Conformational motions and ligand-binding underlying gating and regulation in IP3R channel |
| title_full_unstemmed | Conformational motions and ligand-binding underlying gating and regulation in IP3R channel |
| title_short | Conformational motions and ligand-binding underlying gating and regulation in IP3R channel |
| title_sort | conformational motions and ligand binding underlying gating and regulation in ip3r channel |
| url | https://doi.org/10.1038/s41467-022-34574-1 |
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