Association between inflammatory infiltrates and isolated monosomy 22/del(22q) in meningiomas.
Meningiomas contain highly variable levels of infiltrating tissue macrophages (TiMa) and other immune cells. In this study we investigated the potential association between the number and immunophenotype of inflammatory and other immune cells infiltrating the tumor as evaluated by multiparameter flo...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3788099?pdf=render |
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| author | Patrícia Henriques Domingues Cristina Teodósio Álvaro Otero Pablo Sousa Javier Ortiz María del Carmen García Macias Jesús María Gonçalves Ana Belén Nieto María Celeste Lopes Catarina de Oliveira Alberto Orfao Maria Dolores Tabernero |
| author_facet | Patrícia Henriques Domingues Cristina Teodósio Álvaro Otero Pablo Sousa Javier Ortiz María del Carmen García Macias Jesús María Gonçalves Ana Belén Nieto María Celeste Lopes Catarina de Oliveira Alberto Orfao Maria Dolores Tabernero |
| author_sort | Patrícia Henriques Domingues |
| collection | DOAJ |
| description | Meningiomas contain highly variable levels of infiltrating tissue macrophages (TiMa) and other immune cells. In this study we investigated the potential association between the number and immunophenotype of inflammatory and other immune cells infiltrating the tumor as evaluated by multiparameter flow cytometry, and the clinico-biological, cytogenetic and gene expression profile (GEP) of 75 meningioma patients. Overall, our results showed a close association between the amount and cellular composition of the inflammatory and other immune cell infiltrates and the cytogenetic profile of the tumors. Notably, tumors with isolated monosomy 22/del(22q) showed greater numbers of TiMa, NK cells and (recently)-activated CD69(+) lymphocytes versus meningiomas with diploid and complex karyotypes. In addition, in the former cytogenetic subgroup of meningiomas, tumor-infiltrating TiMa also showed a more activated and functionally mature phenotype, as reflected by a greater fraction of CD69(+), CD63(+), CD16(+) and CD33(+) cells. GEP at the mRNA level showed a unique GEP among meningiomas with an isolated monosomy 22/del(22q) versus all other cases, which consisted of increased expression of genes involved in inflammatory/immune response, associated with an M1 TiMa phenotype. Altogether, these results suggest that loss of expression of specific genes coded in chromosome 22 (e.g. MIF) is closely associated with an increased homing and potentially also anti-tumoral effect of TiMa, which could contribute to explain the better outcome of this specific good-prognosis cytogenetic subgroup of meningiomas. |
| first_indexed | 2024-12-24T00:11:05Z |
| format | Article |
| id | doaj.art-19983ef78d3b408b9310c8e7c5fd92de |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-24T00:11:05Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-19983ef78d3b408b9310c8e7c5fd92de2022-12-21T17:24:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7479810.1371/journal.pone.0074798Association between inflammatory infiltrates and isolated monosomy 22/del(22q) in meningiomas.Patrícia Henriques DominguesCristina TeodósioÁlvaro OteroPablo SousaJavier OrtizMaría del Carmen García MaciasJesús María GonçalvesAna Belén NietoMaría Celeste LopesCatarina de OliveiraAlberto OrfaoMaria Dolores TaberneroMeningiomas contain highly variable levels of infiltrating tissue macrophages (TiMa) and other immune cells. In this study we investigated the potential association between the number and immunophenotype of inflammatory and other immune cells infiltrating the tumor as evaluated by multiparameter flow cytometry, and the clinico-biological, cytogenetic and gene expression profile (GEP) of 75 meningioma patients. Overall, our results showed a close association between the amount and cellular composition of the inflammatory and other immune cell infiltrates and the cytogenetic profile of the tumors. Notably, tumors with isolated monosomy 22/del(22q) showed greater numbers of TiMa, NK cells and (recently)-activated CD69(+) lymphocytes versus meningiomas with diploid and complex karyotypes. In addition, in the former cytogenetic subgroup of meningiomas, tumor-infiltrating TiMa also showed a more activated and functionally mature phenotype, as reflected by a greater fraction of CD69(+), CD63(+), CD16(+) and CD33(+) cells. GEP at the mRNA level showed a unique GEP among meningiomas with an isolated monosomy 22/del(22q) versus all other cases, which consisted of increased expression of genes involved in inflammatory/immune response, associated with an M1 TiMa phenotype. Altogether, these results suggest that loss of expression of specific genes coded in chromosome 22 (e.g. MIF) is closely associated with an increased homing and potentially also anti-tumoral effect of TiMa, which could contribute to explain the better outcome of this specific good-prognosis cytogenetic subgroup of meningiomas.http://europepmc.org/articles/PMC3788099?pdf=render |
| spellingShingle | Patrícia Henriques Domingues Cristina Teodósio Álvaro Otero Pablo Sousa Javier Ortiz María del Carmen García Macias Jesús María Gonçalves Ana Belén Nieto María Celeste Lopes Catarina de Oliveira Alberto Orfao Maria Dolores Tabernero Association between inflammatory infiltrates and isolated monosomy 22/del(22q) in meningiomas. PLoS ONE |
| title | Association between inflammatory infiltrates and isolated monosomy 22/del(22q) in meningiomas. |
| title_full | Association between inflammatory infiltrates and isolated monosomy 22/del(22q) in meningiomas. |
| title_fullStr | Association between inflammatory infiltrates and isolated monosomy 22/del(22q) in meningiomas. |
| title_full_unstemmed | Association between inflammatory infiltrates and isolated monosomy 22/del(22q) in meningiomas. |
| title_short | Association between inflammatory infiltrates and isolated monosomy 22/del(22q) in meningiomas. |
| title_sort | association between inflammatory infiltrates and isolated monosomy 22 del 22q in meningiomas |
| url | http://europepmc.org/articles/PMC3788099?pdf=render |
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