Infection of sheep by Echinococcus multilocularis in Gansu, China: evidence from mitochondrial and nuclear DNA analysis
Abstract Background In the normal life cycle of the parasite (Echinococcus multilocularis) that causes alveolar echinococcosis, domestic and wild carnivores act as definitive hosts, and rodents act as intermediate hosts. The presented study contributes to the research on the distribution and transmi...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | Infectious Diseases of Poverty |
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| Online Access: | https://doi.org/10.1186/s40249-023-01120-0 |
| _version_ | 1797451026892062720 |
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| author | Nigus Abebe Shumuye Li Li Wen-Hui Li Nian-Zhang Zhang Yan-Tao Wu Yao-Dong Wu Wen-Jun Tian Lin-Sheng Zhang Xiao-Feng Nian Guo-Dong Dai Wei-Gang Chen Sheng-Zhi Gao Xue-Qi Tian Jun-Shi Liu Bin Li Nigatu Kebede Bao-Quan Fu Hong-Bin Yan Wan-Zhong Jia |
| author_facet | Nigus Abebe Shumuye Li Li Wen-Hui Li Nian-Zhang Zhang Yan-Tao Wu Yao-Dong Wu Wen-Jun Tian Lin-Sheng Zhang Xiao-Feng Nian Guo-Dong Dai Wei-Gang Chen Sheng-Zhi Gao Xue-Qi Tian Jun-Shi Liu Bin Li Nigatu Kebede Bao-Quan Fu Hong-Bin Yan Wan-Zhong Jia |
| author_sort | Nigus Abebe Shumuye |
| collection | DOAJ |
| description | Abstract Background In the normal life cycle of the parasite (Echinococcus multilocularis) that causes alveolar echinococcosis, domestic and wild carnivores act as definitive hosts, and rodents act as intermediate hosts. The presented study contributes to the research on the distribution and transmission pattern of E. multilocularis in China having identified sheep as an unusual intermediate host taking part in the domestic transmission of alveolar echinococcosis in Gansu Province, China. Methods From 2020 to 2021, nine whitish different cyst-like were collected from the liver of sheep in Gansu Province for examination. A near complete mitochondrial (mt) genome and selected nuclear genes were amplified from the cyst-like lesion for identification. To confirm the status of the specimen, comparative analysis with reference sequences, phylogenetic analysis, and network analysis were performed. Results The isolates displayed ≥ 98.87% similarity to E. multilocularis NADH dehydrogenase sub-unit 1 (nad1) (894 bp) reference sequences deposited in GenBank. Furthermore, amplification of the nad4 and nad2 genes also confirmed all nine samples as E. multilocularis with > 99.30% similarity. Additionally, three nuclear genes, pepck (1545 bp), elp-exons VII and VIII (566 bp), and elp-exon IX (256 bp), were successfully amplified and sequenced for one of the isolates with 98.42% similarity, confirming the isolates were correctly identified as E. multilocularis. Network analysis also correctly placed the isolates with other E. multilocularis. Conclusions As a result of the discovery of E. multilocularis in an unusual intermediate host, which is considered to have the highest zoonotic potential, the result clearly demonstrated the necessity for expanded surveillance in the area. Graphical Abstract |
| first_indexed | 2024-03-09T14:49:01Z |
| format | Article |
| id | doaj.art-19991d5803d846229ec158745b0e7886 |
| institution | Directory Open Access Journal |
| issn | 2049-9957 |
| language | English |
| last_indexed | 2024-03-09T14:49:01Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Infectious Diseases of Poverty |
| spelling | doaj.art-19991d5803d846229ec158745b0e78862023-11-26T14:34:06ZengBMCInfectious Diseases of Poverty2049-99572023-08-0112111210.1186/s40249-023-01120-0Infection of sheep by Echinococcus multilocularis in Gansu, China: evidence from mitochondrial and nuclear DNA analysisNigus Abebe Shumuye0Li Li1Wen-Hui Li2Nian-Zhang Zhang3Yan-Tao Wu4Yao-Dong Wu5Wen-Jun Tian6Lin-Sheng Zhang7Xiao-Feng Nian8Guo-Dong Dai9Wei-Gang Chen10Sheng-Zhi Gao11Xue-Qi Tian12Jun-Shi Liu13Bin Li14Nigatu Kebede15Bao-Quan Fu16Hong-Bin Yan17Wan-Zhong Jia18State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesGansu Animal Centre for Disease Control and PreventionState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesJingyuan County Animal Centre for Disease Control and Prevention, Jingyuan County Animal Husbandry and Veterinary Technical Service CenterJingyuan County Animal Centre for Disease Control and Prevention, Jingyuan County Animal Husbandry and Veterinary Technical Service CenterAklilu Lemma Institute of Pathobiology, Addis Ababa UniversityState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, National Para-Reference Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Lanzhou University, Chinese Academy of Agricultural SciencesAbstract Background In the normal life cycle of the parasite (Echinococcus multilocularis) that causes alveolar echinococcosis, domestic and wild carnivores act as definitive hosts, and rodents act as intermediate hosts. The presented study contributes to the research on the distribution and transmission pattern of E. multilocularis in China having identified sheep as an unusual intermediate host taking part in the domestic transmission of alveolar echinococcosis in Gansu Province, China. Methods From 2020 to 2021, nine whitish different cyst-like were collected from the liver of sheep in Gansu Province for examination. A near complete mitochondrial (mt) genome and selected nuclear genes were amplified from the cyst-like lesion for identification. To confirm the status of the specimen, comparative analysis with reference sequences, phylogenetic analysis, and network analysis were performed. Results The isolates displayed ≥ 98.87% similarity to E. multilocularis NADH dehydrogenase sub-unit 1 (nad1) (894 bp) reference sequences deposited in GenBank. Furthermore, amplification of the nad4 and nad2 genes also confirmed all nine samples as E. multilocularis with > 99.30% similarity. Additionally, three nuclear genes, pepck (1545 bp), elp-exons VII and VIII (566 bp), and elp-exon IX (256 bp), were successfully amplified and sequenced for one of the isolates with 98.42% similarity, confirming the isolates were correctly identified as E. multilocularis. Network analysis also correctly placed the isolates with other E. multilocularis. Conclusions As a result of the discovery of E. multilocularis in an unusual intermediate host, which is considered to have the highest zoonotic potential, the result clearly demonstrated the necessity for expanded surveillance in the area. Graphical Abstracthttps://doi.org/10.1186/s40249-023-01120-0Echinococcus multilocularisChinaSheepLiverPhylogeny |
| spellingShingle | Nigus Abebe Shumuye Li Li Wen-Hui Li Nian-Zhang Zhang Yan-Tao Wu Yao-Dong Wu Wen-Jun Tian Lin-Sheng Zhang Xiao-Feng Nian Guo-Dong Dai Wei-Gang Chen Sheng-Zhi Gao Xue-Qi Tian Jun-Shi Liu Bin Li Nigatu Kebede Bao-Quan Fu Hong-Bin Yan Wan-Zhong Jia Infection of sheep by Echinococcus multilocularis in Gansu, China: evidence from mitochondrial and nuclear DNA analysis Infectious Diseases of Poverty Echinococcus multilocularis China Sheep Liver Phylogeny |
| title | Infection of sheep by Echinococcus multilocularis in Gansu, China: evidence from mitochondrial and nuclear DNA analysis |
| title_full | Infection of sheep by Echinococcus multilocularis in Gansu, China: evidence from mitochondrial and nuclear DNA analysis |
| title_fullStr | Infection of sheep by Echinococcus multilocularis in Gansu, China: evidence from mitochondrial and nuclear DNA analysis |
| title_full_unstemmed | Infection of sheep by Echinococcus multilocularis in Gansu, China: evidence from mitochondrial and nuclear DNA analysis |
| title_short | Infection of sheep by Echinococcus multilocularis in Gansu, China: evidence from mitochondrial and nuclear DNA analysis |
| title_sort | infection of sheep by echinococcus multilocularis in gansu china evidence from mitochondrial and nuclear dna analysis |
| topic | Echinococcus multilocularis China Sheep Liver Phylogeny |
| url | https://doi.org/10.1186/s40249-023-01120-0 |
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