Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract
Chemically modified allergens (allergoids) have found use in both traditional and novel forms of immunotherapy of allergic disorders. Novel forms of immunotherapy include local allergen delivery, via the gastrointestinal tract. This study conveys the gastrointestinal stability of three types of mugw...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2006-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2006/0352-51390609879C.pdf |
| _version_ | 1818878628318937088 |
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| author | Ćirković-Veličković Tanja D. Polović Natalija Đ. Gavrović-Jankulović Marija Đ. Burazer Lidija Đergović-Petrović Danica Vučković Olga Drobnjak Olika Šporčić Zorica Atanasković-Marković Marina Jankov Ratko M. |
| author_facet | Ćirković-Veličković Tanja D. Polović Natalija Đ. Gavrović-Jankulović Marija Đ. Burazer Lidija Đergović-Petrović Danica Vučković Olga Drobnjak Olika Šporčić Zorica Atanasković-Marković Marina Jankov Ratko M. |
| author_sort | Ćirković-Veličković Tanja D. |
| collection | DOAJ |
| description | Chemically modified allergens (allergoids) have found use in both traditional and novel forms of immunotherapy of allergic disorders. Novel forms of immunotherapy include local allergen delivery, via the gastrointestinal tract. This study conveys the gastrointestinal stability of three types of mugwort pollen allergoids under simulated conditions of the gut. Allergoids of the pollen extract of Artemisia vulgaris were obtained by means of potassium cyanate, succinic and maleic anhydride. Gastrointestinal tract conditions (saliva, and gastric fluid) were simulated in accordance with the EU Pharmacopoeia. The biochemical and immunochemical properties of the derivatives following exposure to different conditions were monitored by determining the number of residual amino groups with 2,4,6-trinitrobenzenesulfonic acid, SDS PAGE, immunoblotting and inhibition of mugwort-specific IgE. Exposure to saliva fluid for 2 min did not influence the biochemical and immunochemical properties of the derivatives. In the very acidic conditions of the simulated gastric fluid, the degree of demaleylation and desuccinylation, even after 4 h exposure, was low, ranging from 10 to 30 %. The digestion patterns with pepsin proceeded rapidly in both the unmodified and modified samples. In all four cases, a highly resistant IgE-binding protein the Mwof which was about 28-35 kD, was present. Within the physiological conditions, no new IgE binding epitopes were revealed, as demonstrated by immunoblot and CAP inhibition of the mugwort specific IgE binding. An important conclusion of this study is the stability of the modified derivatives in the gastrointestinal tract of patients, within physiological conditions. The means that they are suitable for use in much higher concentrations in local forms of immunotherapy than unmodified ones. |
| first_indexed | 2024-12-19T14:17:12Z |
| format | Article |
| id | doaj.art-199b7bf0951c420e8f1cb2548368f544 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-12-19T14:17:12Z |
| publishDate | 2006-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-199b7bf0951c420e8f1cb2548368f5442022-12-21T20:17:56ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212006-01-01718-987988810.2298/JSC0609879C0352-51390609879CArtemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tractĆirković-Veličković Tanja D.0Polović Natalija Đ.1Gavrović-Jankulović Marija Đ.2Burazer Lidija3Đergović-Petrović Danica4Vučković Olga5Drobnjak Olika6Šporčić Zorica7Atanasković-Marković Marina8Jankov Ratko M.9Hemijski fakultet, BeogradHemijski fakultet, BeogradHemijski fakultet, BeogradInstitut za imunologiju i virusologiju, Torlak, BeogradInstitut za imunologiju i virusologiju, Torlak, BeogradInstitut za imunologiju i virusologiju, Torlak, BeogradUniverzitetski medicinski centar 'Bežanijska kosa', BeogradUniverzitetski medicinski centar 'Bežanijska kosa', BeogradUniverzitetska dečja bolnica, BeogradHemijski fakultet, BeogradChemically modified allergens (allergoids) have found use in both traditional and novel forms of immunotherapy of allergic disorders. Novel forms of immunotherapy include local allergen delivery, via the gastrointestinal tract. This study conveys the gastrointestinal stability of three types of mugwort pollen allergoids under simulated conditions of the gut. Allergoids of the pollen extract of Artemisia vulgaris were obtained by means of potassium cyanate, succinic and maleic anhydride. Gastrointestinal tract conditions (saliva, and gastric fluid) were simulated in accordance with the EU Pharmacopoeia. The biochemical and immunochemical properties of the derivatives following exposure to different conditions were monitored by determining the number of residual amino groups with 2,4,6-trinitrobenzenesulfonic acid, SDS PAGE, immunoblotting and inhibition of mugwort-specific IgE. Exposure to saliva fluid for 2 min did not influence the biochemical and immunochemical properties of the derivatives. In the very acidic conditions of the simulated gastric fluid, the degree of demaleylation and desuccinylation, even after 4 h exposure, was low, ranging from 10 to 30 %. The digestion patterns with pepsin proceeded rapidly in both the unmodified and modified samples. In all four cases, a highly resistant IgE-binding protein the Mwof which was about 28-35 kD, was present. Within the physiological conditions, no new IgE binding epitopes were revealed, as demonstrated by immunoblot and CAP inhibition of the mugwort specific IgE binding. An important conclusion of this study is the stability of the modified derivatives in the gastrointestinal tract of patients, within physiological conditions. The means that they are suitable for use in much higher concentrations in local forms of immunotherapy than unmodified ones.http://www.doiserbia.nb.rs/img/doi/0352-5139/2006/0352-51390609879C.pdfallergoidartemisia vulgarismugwort pollenchemical modificationdigestionmaleic anhydridepotassium cyanatesuccinic anhydride |
| spellingShingle | Ćirković-Veličković Tanja D. Polović Natalija Đ. Gavrović-Jankulović Marija Đ. Burazer Lidija Đergović-Petrović Danica Vučković Olga Drobnjak Olika Šporčić Zorica Atanasković-Marković Marina Jankov Ratko M. Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract Journal of the Serbian Chemical Society allergoid artemisia vulgaris mugwort pollen chemical modification digestion maleic anhydride potassium cyanate succinic anhydride |
| title | Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract |
| title_full | Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract |
| title_fullStr | Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract |
| title_full_unstemmed | Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract |
| title_short | Artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract |
| title_sort | artemisia vulgaris pollen allergoids digestibility in the simulated conditions of the gastrointestinal tract |
| topic | allergoid artemisia vulgaris mugwort pollen chemical modification digestion maleic anhydride potassium cyanate succinic anhydride |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2006/0352-51390609879C.pdf |
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