The Effect of Cryopreserved Human Placental Tissues on Biofilm Formation of Wound-Associated Pathogens
Biofilm, a community of bacteria, is tolerant to antimicrobial agents and ubiquitous in chronic wounds. In a chronic DFU (Diabetic Foot Ulcers) clinical trial, the use of a human cryopreserved viable amniotic membrane (CVAM) resulted in a high rate of wound closure and reduction of wound-related inf...
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| Format: | Article |
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MDPI AG
2018-01-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | http://www.mdpi.com/2079-4983/9/1/3 |
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| author | Yong Mao Anya Singh-Varma Tyler Hoffman Sandeep Dhall Alla Danilkovitch Joachim Kohn |
| author_facet | Yong Mao Anya Singh-Varma Tyler Hoffman Sandeep Dhall Alla Danilkovitch Joachim Kohn |
| author_sort | Yong Mao |
| collection | DOAJ |
| description | Biofilm, a community of bacteria, is tolerant to antimicrobial agents and ubiquitous in chronic wounds. In a chronic DFU (Diabetic Foot Ulcers) clinical trial, the use of a human cryopreserved viable amniotic membrane (CVAM) resulted in a high rate of wound closure and reduction of wound-related infections. Our previous study demonstrated that CVAM possesses intrinsic antimicrobial activity against a spectrum of wound-associated bacteria under planktonic culture conditions. In this study, we evaluated the effect of CVAM and cryopreserved viable umbilical tissue (CVUT) on biofilm formation of S. aureus and P. aeruginosa, the two most prominent pathogens associated with chronic wounds. Firstly, we showed that, like CVAM, CVUT released antibacterial activity against multiple bacterial pathogens and the devitalization of CVUT reduced its antibacterial activity. The biofilm formation was then measured using a high throughput method and an ex vivo porcine dermal tissue model. We demonstrate that the formation of biofilm was significantly reduced in the presence of CVAM- or CVUT-derived conditioned media compared to control assay medium. The formation of P. aeruginosa biofilm on CVAM-conditioned medium saturated porcine dermal tissues was reduced 97% compared with the biofilm formation on the control medium saturated dermal tissues. The formation of S. auerus biofilm on CVUT-conditioned medium saturated dermal tissues was reduced 72% compared with the biofilm formation on the control tissues. This study is the first to show that human cryopreserved viable placental tissues release factors that inhibit biofilm formation. Our results provide an explanation for the in vivo observation of their ability to support wound healing. |
| first_indexed | 2024-12-10T07:22:22Z |
| format | Article |
| id | doaj.art-199ca33a05b84d498398d10779411637 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-12-10T07:22:22Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-199ca33a05b84d498398d107794116372022-12-22T01:57:47ZengMDPI AGJournal of Functional Biomaterials2079-49832018-01-0191310.3390/jfb9010003jfb9010003The Effect of Cryopreserved Human Placental Tissues on Biofilm Formation of Wound-Associated PathogensYong Mao0Anya Singh-Varma1Tyler Hoffman2Sandeep Dhall3Alla Danilkovitch4Joachim Kohn5New Jersey Center for Biomaterials, Rutgers University, 145 Bevier Rd., Piscataway, NJ 08854, USANew Jersey Center for Biomaterials, Rutgers University, 145 Bevier Rd., Piscataway, NJ 08854, USAOsiris Therapeutics, Inc., Columbia, MD 21046, USAOsiris Therapeutics, Inc., Columbia, MD 21046, USAOsiris Therapeutics, Inc., Columbia, MD 21046, USANew Jersey Center for Biomaterials, Rutgers University, 145 Bevier Rd., Piscataway, NJ 08854, USABiofilm, a community of bacteria, is tolerant to antimicrobial agents and ubiquitous in chronic wounds. In a chronic DFU (Diabetic Foot Ulcers) clinical trial, the use of a human cryopreserved viable amniotic membrane (CVAM) resulted in a high rate of wound closure and reduction of wound-related infections. Our previous study demonstrated that CVAM possesses intrinsic antimicrobial activity against a spectrum of wound-associated bacteria under planktonic culture conditions. In this study, we evaluated the effect of CVAM and cryopreserved viable umbilical tissue (CVUT) on biofilm formation of S. aureus and P. aeruginosa, the two most prominent pathogens associated with chronic wounds. Firstly, we showed that, like CVAM, CVUT released antibacterial activity against multiple bacterial pathogens and the devitalization of CVUT reduced its antibacterial activity. The biofilm formation was then measured using a high throughput method and an ex vivo porcine dermal tissue model. We demonstrate that the formation of biofilm was significantly reduced in the presence of CVAM- or CVUT-derived conditioned media compared to control assay medium. The formation of P. aeruginosa biofilm on CVAM-conditioned medium saturated porcine dermal tissues was reduced 97% compared with the biofilm formation on the control medium saturated dermal tissues. The formation of S. auerus biofilm on CVUT-conditioned medium saturated dermal tissues was reduced 72% compared with the biofilm formation on the control tissues. This study is the first to show that human cryopreserved viable placental tissues release factors that inhibit biofilm formation. Our results provide an explanation for the in vivo observation of their ability to support wound healing.http://www.mdpi.com/2079-4983/9/1/3biofilmchronic woundcryopreserved amniotic membranecryopreserved umbilical cord tissueantibacterial |
| spellingShingle | Yong Mao Anya Singh-Varma Tyler Hoffman Sandeep Dhall Alla Danilkovitch Joachim Kohn The Effect of Cryopreserved Human Placental Tissues on Biofilm Formation of Wound-Associated Pathogens Journal of Functional Biomaterials biofilm chronic wound cryopreserved amniotic membrane cryopreserved umbilical cord tissue antibacterial |
| title | The Effect of Cryopreserved Human Placental Tissues on Biofilm Formation of Wound-Associated Pathogens |
| title_full | The Effect of Cryopreserved Human Placental Tissues on Biofilm Formation of Wound-Associated Pathogens |
| title_fullStr | The Effect of Cryopreserved Human Placental Tissues on Biofilm Formation of Wound-Associated Pathogens |
| title_full_unstemmed | The Effect of Cryopreserved Human Placental Tissues on Biofilm Formation of Wound-Associated Pathogens |
| title_short | The Effect of Cryopreserved Human Placental Tissues on Biofilm Formation of Wound-Associated Pathogens |
| title_sort | effect of cryopreserved human placental tissues on biofilm formation of wound associated pathogens |
| topic | biofilm chronic wound cryopreserved amniotic membrane cryopreserved umbilical cord tissue antibacterial |
| url | http://www.mdpi.com/2079-4983/9/1/3 |
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