EFFECT OF LACTOBACILLI EXOPOLYSACCHARIDES ON PHAGOCYTE AND CYTOKINE ACTIVITY IN VITRO AND IN ANIMAL ORGANISM DURING INFECTIOUS PROCESS MODELING
Aim. Study the effect of lactobacilli exopolysaccharides (EPS) on cytokine and phagocyte activity in vitro and in mice organism during modelling of an infectious process. Materials and methods. Lactobacillus delbrueckiisubsp. delbrueckii B-1596 (laksaran 1596), L. delbrueckii B-1936 (laksaran 1936)...
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2015-10-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.crie.ru/jour/article/view/14126 |
| _version_ | 1797782187038212096 |
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| author | E. A Gorelnikova L. V Karpunina |
| author_facet | E. A Gorelnikova L. V Karpunina |
| author_sort | E. A Gorelnikova |
| collection | DOAJ |
| description | Aim. Study the effect of lactobacilli exopolysaccharides (EPS) on cytokine and phagocyte activity in vitro and in mice organism during modelling of an infectious process. Materials and methods. Lactobacillus delbrueckiisubsp. delbrueckii B-1596 (laksaran 1596), L. delbrueckii B-1936 (laksaran 1936) and L. delbrueckii ssp. bulgaricus (laksaran Z) were used in the study. EPS were administered into white mice 1 hour after the Staphylococcus aureus 209-P infection. Index of phagocyte completion and index of killing activation (IKA) were calculated during phagocyte activity study. IL-1a, TNF-α, IFN-γ and IL-4 cytokine content was determined in blood sera and macrophage supernatants. Results. Laksaran 1596, 1936 and Z had ambiguous effect on cytokine production. Laksaran Z and 1936, 6 hours after mice infection increased IL-1 content in blood sera. Laksaran Z had the most pronounced effect on macrophages, resulting in an increase of active macrophages, facilitating increased digestion of S. aureus 209-Р and IKA increase, stimulated cytokine production. Conclusion. The results obtained allow to speak about a possibility of using laksaran Z as a prophylaxis immune modulating preparation for correction of animal cytokine status. |
| first_indexed | 2024-03-13T00:07:27Z |
| format | Article |
| id | doaj.art-199ceea809994f4eaa3c973c7bda7ad6 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-03-13T00:07:27Z |
| publishDate | 2015-10-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-199ceea809994f4eaa3c973c7bda7ad62023-07-12T20:19:07ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132015-10-0192544502551EFFECT OF LACTOBACILLI EXOPOLYSACCHARIDES ON PHAGOCYTE AND CYTOKINE ACTIVITY IN VITRO AND IN ANIMAL ORGANISM DURING INFECTIOUS PROCESS MODELINGE. A Gorelnikova0L. V Karpunina1Vavilov State Agrarian UniversityVavilov State Agrarian UniversityAim. Study the effect of lactobacilli exopolysaccharides (EPS) on cytokine and phagocyte activity in vitro and in mice organism during modelling of an infectious process. Materials and methods. Lactobacillus delbrueckiisubsp. delbrueckii B-1596 (laksaran 1596), L. delbrueckii B-1936 (laksaran 1936) and L. delbrueckii ssp. bulgaricus (laksaran Z) were used in the study. EPS were administered into white mice 1 hour after the Staphylococcus aureus 209-P infection. Index of phagocyte completion and index of killing activation (IKA) were calculated during phagocyte activity study. IL-1a, TNF-α, IFN-γ and IL-4 cytokine content was determined in blood sera and macrophage supernatants. Results. Laksaran 1596, 1936 and Z had ambiguous effect on cytokine production. Laksaran Z and 1936, 6 hours after mice infection increased IL-1 content in blood sera. Laksaran Z had the most pronounced effect on macrophages, resulting in an increase of active macrophages, facilitating increased digestion of S. aureus 209-Р and IKA increase, stimulated cytokine production. Conclusion. The results obtained allow to speak about a possibility of using laksaran Z as a prophylaxis immune modulating preparation for correction of animal cytokine status.https://microbiol.crie.ru/jour/article/view/14126exopolysaccharideslactobacillilaksaransphagocyte and cytokine activity |
| spellingShingle | E. A Gorelnikova L. V Karpunina EFFECT OF LACTOBACILLI EXOPOLYSACCHARIDES ON PHAGOCYTE AND CYTOKINE ACTIVITY IN VITRO AND IN ANIMAL ORGANISM DURING INFECTIOUS PROCESS MODELING Журнал микробиологии, эпидемиологии и иммунобиологии exopolysaccharides lactobacilli laksarans phagocyte and cytokine activity |
| title | EFFECT OF LACTOBACILLI EXOPOLYSACCHARIDES ON PHAGOCYTE AND CYTOKINE ACTIVITY IN VITRO AND IN ANIMAL ORGANISM DURING INFECTIOUS PROCESS MODELING |
| title_full | EFFECT OF LACTOBACILLI EXOPOLYSACCHARIDES ON PHAGOCYTE AND CYTOKINE ACTIVITY IN VITRO AND IN ANIMAL ORGANISM DURING INFECTIOUS PROCESS MODELING |
| title_fullStr | EFFECT OF LACTOBACILLI EXOPOLYSACCHARIDES ON PHAGOCYTE AND CYTOKINE ACTIVITY IN VITRO AND IN ANIMAL ORGANISM DURING INFECTIOUS PROCESS MODELING |
| title_full_unstemmed | EFFECT OF LACTOBACILLI EXOPOLYSACCHARIDES ON PHAGOCYTE AND CYTOKINE ACTIVITY IN VITRO AND IN ANIMAL ORGANISM DURING INFECTIOUS PROCESS MODELING |
| title_short | EFFECT OF LACTOBACILLI EXOPOLYSACCHARIDES ON PHAGOCYTE AND CYTOKINE ACTIVITY IN VITRO AND IN ANIMAL ORGANISM DURING INFECTIOUS PROCESS MODELING |
| title_sort | effect of lactobacilli exopolysaccharides on phagocyte and cytokine activity in vitro and in animal organism during infectious process modeling |
| topic | exopolysaccharides lactobacilli laksarans phagocyte and cytokine activity |
| url | https://microbiol.crie.ru/jour/article/view/14126 |
| work_keys_str_mv | AT eagorelnikova effectoflactobacilliexopolysaccharidesonphagocyteandcytokineactivityinvitroandinanimalorganismduringinfectiousprocessmodeling AT lvkarpunina effectoflactobacilliexopolysaccharidesonphagocyteandcytokineactivityinvitroandinanimalorganismduringinfectiousprocessmodeling |