Enhancing resin-dentin bond durability using a novel mussel-inspired monomer
Numerous approaches have been developed to improve the resin-dentin bond performance, among which the bio-application of mussel-derived compounds have drawn great attention recently. To assess the performance of N-(3,4-dihydroxyphenethyl)methacrylamide (DMA), a mussel-derived compound, as a function...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S259000642100082X |
| _version_ | 1818353014958718976 |
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| author | Kang Li Chenmin Yao Yuhong Sun Kun Wang Xiangtao Wang Zhengzhi Wang James Kit Hon Tsoi Cui Huang Cynthia Kar Yung Yiu |
| author_facet | Kang Li Chenmin Yao Yuhong Sun Kun Wang Xiangtao Wang Zhengzhi Wang James Kit Hon Tsoi Cui Huang Cynthia Kar Yung Yiu |
| author_sort | Kang Li |
| collection | DOAJ |
| description | Numerous approaches have been developed to improve the resin-dentin bond performance, among which the bio-application of mussel-derived compounds have drawn great attention recently. To assess the performance of N-(3,4-dihydroxyphenethyl)methacrylamide (DMA), a mussel-derived compound, as a functional monomer in dental adhesive, its potential property to cross-link with dentin collagen and polymerize with adhesive will first be evaluated by transmission electron microscopy (TEM), attenuated total reflectance technique of Fourier transform infrared (ATR-FTIR), and atomic force microscopy (AFM) via Peakforce QNM mode. After validating the influence of DMA on collagen and adhesive separately, the overall performance of DMA/ethanol solution as a primer in dentin bonding was examined using micro-tensile bond strength (μTBS) testing, fracture pattern observation, and nanoleakage evaluation both immediately and after 10,000 times thermocycling aging. The inhibitory effect of DMA on endogenous metalloproteinases (MMPs) was evaluated by in situ zymography using confocal laser scanning microscopy (CLSM) and the cytotoxicity of DMA was evaluated using cell counting kit-8. Results demonstrated that DMA successfully cross-linked with dentin collagen via non-covalent bonds and had no influence on the polymerization and mechanical properties of the adhesive. Furthermore, even after 10,000 times thermocycling aging, the μTBS and nanoleakage expression of the DMA-treated groups showed no significant change compared with their immediate values. In situ zymography revealed reduced endogenous proteolytic activities after the application of DMA, and no cytotoxicity effect was observed for DMA concentration up to 25 μmol/L. Thus, DMA could be used as a novel, biocompatible functional monomer in dentin bonding. |
| first_indexed | 2024-12-13T19:02:48Z |
| format | Article |
| id | doaj.art-199f6abbd5b9470c9bd91642d6a2c91e |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-12-13T19:02:48Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-199f6abbd5b9470c9bd91642d6a2c91e2022-12-21T23:34:38ZengElsevierMaterials Today Bio2590-00642021-09-0112100174Enhancing resin-dentin bond durability using a novel mussel-inspired monomerKang Li0Chenmin Yao1Yuhong Sun2Kun Wang3Xiangtao Wang4Zhengzhi Wang5James Kit Hon Tsoi6Cui Huang7Cynthia Kar Yung Yiu8Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Sai Ying Pun, Hong Kong, 999077, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, ChinaCenter of Stomatology, Peking University Shenzhen Hospital, Shenzhen, 518036, ChinaDepartment of Engineering Mechanics, School of Civil Engineering, Wuhan University, Wuhan, 430072, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, ChinaDepartment of Engineering Mechanics, School of Civil Engineering, Wuhan University, Wuhan, 430072, ChinaDental Materials Science, Applied Oral Sciences, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Sai Ying Pun, Hong Kong, 999077, ChinaThe State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China; Corresponding author. School and Hospital of Stomatology, Wuhan University, #237 Luoyu Road, Hongshan District, Wuhan, 430079, China.Paediatric Dentistry and Orthodontics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Sai Ying Pun, Hong Kong, 999077, China; Corresponding author. Paediatric Dentistry and Orthodontics, 2nd Floor, Prince Philip Dental Hospital, 34-Hospital Road, Sai Ying Pun, Hong Kong (SAR), China.Numerous approaches have been developed to improve the resin-dentin bond performance, among which the bio-application of mussel-derived compounds have drawn great attention recently. To assess the performance of N-(3,4-dihydroxyphenethyl)methacrylamide (DMA), a mussel-derived compound, as a functional monomer in dental adhesive, its potential property to cross-link with dentin collagen and polymerize with adhesive will first be evaluated by transmission electron microscopy (TEM), attenuated total reflectance technique of Fourier transform infrared (ATR-FTIR), and atomic force microscopy (AFM) via Peakforce QNM mode. After validating the influence of DMA on collagen and adhesive separately, the overall performance of DMA/ethanol solution as a primer in dentin bonding was examined using micro-tensile bond strength (μTBS) testing, fracture pattern observation, and nanoleakage evaluation both immediately and after 10,000 times thermocycling aging. The inhibitory effect of DMA on endogenous metalloproteinases (MMPs) was evaluated by in situ zymography using confocal laser scanning microscopy (CLSM) and the cytotoxicity of DMA was evaluated using cell counting kit-8. Results demonstrated that DMA successfully cross-linked with dentin collagen via non-covalent bonds and had no influence on the polymerization and mechanical properties of the adhesive. Furthermore, even after 10,000 times thermocycling aging, the μTBS and nanoleakage expression of the DMA-treated groups showed no significant change compared with their immediate values. In situ zymography revealed reduced endogenous proteolytic activities after the application of DMA, and no cytotoxicity effect was observed for DMA concentration up to 25 μmol/L. Thus, DMA could be used as a novel, biocompatible functional monomer in dentin bonding.http://www.sciencedirect.com/science/article/pii/S259000642100082XMusselMonomerCollagenN-(3,4-dihydroxyphenethyl)methacrylamideBond durabilityResin-dentin interface |
| spellingShingle | Kang Li Chenmin Yao Yuhong Sun Kun Wang Xiangtao Wang Zhengzhi Wang James Kit Hon Tsoi Cui Huang Cynthia Kar Yung Yiu Enhancing resin-dentin bond durability using a novel mussel-inspired monomer Materials Today Bio Mussel Monomer Collagen N-(3,4-dihydroxyphenethyl)methacrylamide Bond durability Resin-dentin interface |
| title | Enhancing resin-dentin bond durability using a novel mussel-inspired monomer |
| title_full | Enhancing resin-dentin bond durability using a novel mussel-inspired monomer |
| title_fullStr | Enhancing resin-dentin bond durability using a novel mussel-inspired monomer |
| title_full_unstemmed | Enhancing resin-dentin bond durability using a novel mussel-inspired monomer |
| title_short | Enhancing resin-dentin bond durability using a novel mussel-inspired monomer |
| title_sort | enhancing resin dentin bond durability using a novel mussel inspired monomer |
| topic | Mussel Monomer Collagen N-(3,4-dihydroxyphenethyl)methacrylamide Bond durability Resin-dentin interface |
| url | http://www.sciencedirect.com/science/article/pii/S259000642100082X |
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