Neuroprotective effects of arbutin against oxygen and glucose deprivation-induced oxidative stress and neuroinflammation in rat cortical neurons
In this study, the neuroprotective potential of arbutin (100 µmol L−1) pre-treatment and post-treatment against oxygen/ glucose deprivation (OGD) and reoxygenation (R) induced ischemic injury in cultured rat cortical neurons was explored. The OGD (60 min) and reoxygenation (24 h) treatment significa...
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| Format: | Article |
| Language: | English |
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Sciendo
2022-03-01
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| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2022-0002 |
| _version_ | 1819000865958133760 |
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| author | Tan Jingjing Yadav Manoj Kumar Devi Sushma Kumar Manish |
| author_facet | Tan Jingjing Yadav Manoj Kumar Devi Sushma Kumar Manish |
| author_sort | Tan Jingjing |
| collection | DOAJ |
| description | In this study, the neuroprotective potential of arbutin (100 µmol L−1) pre-treatment and post-treatment against oxygen/ glucose deprivation (OGD) and reoxygenation (R) induced ischemic injury in cultured rat cortical neurons was explored. The OGD (60 min) and reoxygenation (24 h) treatment significantly (p < 0.001) compromised the antioxidant defence in cultured neurons. Subsequently, an increase (p < 0.001) in lipid peroxidation and inflammatory cytokines (tumour necrosis factor-α and nuclear factor kappa-B) declined neuron survival. In pre- and post-condition experiments, treatment with arbutin enhanced both survival (p < 0.01) and integrity (p < 0.05) of cultured neurons. Results showed that arbutin protects (p < 0.05) against peroxidative changes, inflammation, and enhanced the antioxidant activity (e.g., glutathione, superoxide dismutase and catalase) in cultured neurons subjected to OGD/R. It can be inferred that arbutin could protect against ischemic injuries and stroke. The anti-ischemic activity of arbutin can arrest post-stroke damage to the brain. |
| first_indexed | 2024-12-20T22:40:07Z |
| format | Article |
| id | doaj.art-199f9abd6056456fa36a0d7f6db445d9 |
| institution | Directory Open Access Journal |
| issn | 1846-9558 |
| language | English |
| last_indexed | 2024-12-20T22:40:07Z |
| publishDate | 2022-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj.art-199f9abd6056456fa36a0d7f6db445d92022-12-21T19:24:29ZengSciendoActa Pharmaceutica1846-95582022-03-0172112313410.2478/acph-2022-0002Neuroprotective effects of arbutin against oxygen and glucose deprivation-induced oxidative stress and neuroinflammation in rat cortical neuronsTan Jingjing0Yadav Manoj Kumar1Devi Sushma2Kumar Manish3Department of Anesthesia and Perioperative Medicine, Shanghai Fourth People’s Hospital, Affiliated to Tongji University, Shanghai200081ChinaDepartment of Pharmacology, Swift School of Pharmacy, Ghaggar Sarai Rajpura (Patiala)Punjab – 140401, IndiaDepartment of Pharmacology, Swift School of Pharmacy, Ghaggar Sarai Rajpura (Patiala)Punjab – 140401, IndiaDepartment of Pharmacology, Swift School of Pharmacy, Ghaggar Sarai Rajpura (Patiala)Punjab – 140401, IndiaIn this study, the neuroprotective potential of arbutin (100 µmol L−1) pre-treatment and post-treatment against oxygen/ glucose deprivation (OGD) and reoxygenation (R) induced ischemic injury in cultured rat cortical neurons was explored. The OGD (60 min) and reoxygenation (24 h) treatment significantly (p < 0.001) compromised the antioxidant defence in cultured neurons. Subsequently, an increase (p < 0.001) in lipid peroxidation and inflammatory cytokines (tumour necrosis factor-α and nuclear factor kappa-B) declined neuron survival. In pre- and post-condition experiments, treatment with arbutin enhanced both survival (p < 0.01) and integrity (p < 0.05) of cultured neurons. Results showed that arbutin protects (p < 0.05) against peroxidative changes, inflammation, and enhanced the antioxidant activity (e.g., glutathione, superoxide dismutase and catalase) in cultured neurons subjected to OGD/R. It can be inferred that arbutin could protect against ischemic injuries and stroke. The anti-ischemic activity of arbutin can arrest post-stroke damage to the brain.https://doi.org/10.2478/acph-2022-0002arbutincerebral ischemiainflammationoxidative stress |
| spellingShingle | Tan Jingjing Yadav Manoj Kumar Devi Sushma Kumar Manish Neuroprotective effects of arbutin against oxygen and glucose deprivation-induced oxidative stress and neuroinflammation in rat cortical neurons Acta Pharmaceutica arbutin cerebral ischemia inflammation oxidative stress |
| title | Neuroprotective effects of arbutin against oxygen and glucose deprivation-induced oxidative stress and neuroinflammation in rat cortical neurons |
| title_full | Neuroprotective effects of arbutin against oxygen and glucose deprivation-induced oxidative stress and neuroinflammation in rat cortical neurons |
| title_fullStr | Neuroprotective effects of arbutin against oxygen and glucose deprivation-induced oxidative stress and neuroinflammation in rat cortical neurons |
| title_full_unstemmed | Neuroprotective effects of arbutin against oxygen and glucose deprivation-induced oxidative stress and neuroinflammation in rat cortical neurons |
| title_short | Neuroprotective effects of arbutin against oxygen and glucose deprivation-induced oxidative stress and neuroinflammation in rat cortical neurons |
| title_sort | neuroprotective effects of arbutin against oxygen and glucose deprivation induced oxidative stress and neuroinflammation in rat cortical neurons |
| topic | arbutin cerebral ischemia inflammation oxidative stress |
| url | https://doi.org/10.2478/acph-2022-0002 |
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